Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/8940 |
Resumo: | Previous studies suggested that the Angiotensin-(1-7) [(Ang-(1-7)] is able to modulate the cardiac sympathetic control and beta-adrenergic sensitivity. However, whether or not Ang-(1- 7) modulates the cholinergic activity in the heart remains unknown. The aim of this study was to evaluate the influence of Ang-(1-7) upon cholinergic sensitivity of hearts from normotensive and hypertensive rats. Wistar and Spontaneously Hypertensive Rats (SHR) were anesthetized with urethane and underwent catheterization of femoral artery and left ventricle to record the arterial and intraventricular pressure, respectively. Following, a dose-response curve of acetylcholine (ACh, 10, 20, 40 and 80 ng/Kg, i.v. into femoral vein) was performed in the absence or presence of Ang-(1-7) (7 x 10-12 mol/min), Mas receptor antagonist A-779 (7 x 10-11 mol/min) or Ang-(1-7)+A-779. Isolated hearts were perfused according to the Langendorff technique. Increasing concentrations of ACh (10-7 to 10-5 mol/L) were added to the hearts in absence or presence of Ang-(1-7), (2 x 10-11 mol/L), A-779, (2 x 10-10 mol/L), Ang-(1-7)+A-779, MrgD receptor antagonist, D-PRO (2 x 10-10 mol/L) or D-PRO+Ang-(1-7). ACh-induced vasorelaxation was assessed in absence or presence of Ang-(1-7) (2 x 10-11 mol/L or 2 x 10-10 mol/L). Ang-(1-7) attenuated the effect of ACh in decreasing the intraventricular systolic, dP/dt max and dP/dt min in anesthetized Wistar and SHR. These effects were blocked by A-779. Ang-(1-7) did not change the amplitude of the hypotensive effect evoked by ACh in Wistar or SHRs. In isolated hearts, Ang-(1-7) also attenuated the reduction of the intraventricular systolic pressure, dP/dt max and dP/dt min evoked by ACh. A-779 blocked the Ang-(1-7) effects in hearts from Wistar. A-779 or D-PRO did not modify the effects of Ang-(1-7) in hearts from SHR, but in presence of D-PRO, Ang-(1-7) effects were equipotent. Ang-(1-7) attenuated the vasorelaxation induced by ACh in aorta from SHR by only in SHR group. These data suggest that Ang-(1-7) exerts differential modulation of cardiac cholinergic sensitivity during experimental primary hypertension, which is independent on blood pressure. |
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Castro, Carlos Henrique dehttp://lattes.cnpq.br/6354834854727314Custódio, Carlos Henrique Xavierhttp://lattes.cnpq.br/0207928273284808Pansani, Aline PriscilaPedrino, Gustavo RodriguesCastro, Carlos Henrique deCustódio, Carlos Henrique Xavierhttp://lattes.cnpq.br/6231711118811807Pontes, Carolina Nobre Ribeiro2018-10-03T11:46:08Z2018-03-02PONTES, C. N. R. Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos. 2018. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/8940Previous studies suggested that the Angiotensin-(1-7) [(Ang-(1-7)] is able to modulate the cardiac sympathetic control and beta-adrenergic sensitivity. However, whether or not Ang-(1- 7) modulates the cholinergic activity in the heart remains unknown. The aim of this study was to evaluate the influence of Ang-(1-7) upon cholinergic sensitivity of hearts from normotensive and hypertensive rats. Wistar and Spontaneously Hypertensive Rats (SHR) were anesthetized with urethane and underwent catheterization of femoral artery and left ventricle to record the arterial and intraventricular pressure, respectively. Following, a dose-response curve of acetylcholine (ACh, 10, 20, 40 and 80 ng/Kg, i.v. into femoral vein) was performed in the absence or presence of Ang-(1-7) (7 x 10-12 mol/min), Mas receptor antagonist A-779 (7 x 10-11 mol/min) or Ang-(1-7)+A-779. Isolated hearts were perfused according to the Langendorff technique. Increasing concentrations of ACh (10-7 to 10-5 mol/L) were added to the hearts in absence or presence of Ang-(1-7), (2 x 10-11 mol/L), A-779, (2 x 10-10 mol/L), Ang-(1-7)+A-779, MrgD receptor antagonist, D-PRO (2 x 10-10 mol/L) or D-PRO+Ang-(1-7). ACh-induced vasorelaxation was assessed in absence or presence of Ang-(1-7) (2 x 10-11 mol/L or 2 x 10-10 mol/L). Ang-(1-7) attenuated the effect of ACh in decreasing the intraventricular systolic, dP/dt max and dP/dt min in anesthetized Wistar and SHR. These effects were blocked by A-779. Ang-(1-7) did not change the amplitude of the hypotensive effect evoked by ACh in Wistar or SHRs. In isolated hearts, Ang-(1-7) also attenuated the reduction of the intraventricular systolic pressure, dP/dt max and dP/dt min evoked by ACh. A-779 blocked the Ang-(1-7) effects in hearts from Wistar. A-779 or D-PRO did not modify the effects of Ang-(1-7) in hearts from SHR, but in presence of D-PRO, Ang-(1-7) effects were equipotent. Ang-(1-7) attenuated the vasorelaxation induced by ACh in aorta from SHR by only in SHR group. These data suggest that Ang-(1-7) exerts differential modulation of cardiac cholinergic sensitivity during experimental primary hypertension, which is independent on blood pressure.Estudos prévios sugerem que a Angiotensina-(1-7) [(Ang-(1-7)] é capaz de modular o controle simpático cardíaco e sensibilidade beta-adrenérgica. Entretanto, ainda não se sabe se a Ang-(1-7) consegue modular a atividade colinérgica no coração. O objetivo deste estudo foiavaliar a influência da Ang-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos. Wistar e Ratos Espontaneamente Hipertensos (SHR) foram anestesiados com uretano e submetidos à canulação de artéria femoral e ventrículo esquerdo cardíaco para registro de pressão arterial e intraventricular, respectivamente. Em seguida, foi realizada uma curva dose-resposta de acetilcolina (ACh, 10, 20, 40 e 80 ng/Kg, i.v.) por infusão pela veia femoral. A infusão ocorreu na presença e ausência de Ang-(1-7) (7 x 10-12 mol/min), do antagonista do receptor Mas, A-779 (7 x 10-11 mol/min) ou de Ang-(1-7)+A-779. Os corações isolados foram perfundidos de acordo com a técnica de Langendorff e concentrações crescentes de ACh (10-7 a 10-5 mol/L) foram adicionadas aos corações na presença ou ausência de Ang-(1-7), (2 x 10-11 mol/L), A-779, (2 x 10-10 mol/L), Ang-(1-7)+A-779, antagonista do receptor MrgD, D-PRO (2 x 10-10 mol/L) ou D-PRO+Ang-(1-7). O vasorrelaxamento induzido pela ACh foi mensurado na presença ou ausência da Ang-(1-7) (2 x 10-11 mol/L ou 2 x 10-10 mol/L). Em Wistar e SHR anestesiados, a Ang-(1-7) atenuou o efeito da ACh na queda da pressão intraventricular sistólica, dP/dt máx, e dP/dt mín. Estes efeitos foram bloqueados pelo A-779. A Ang-(1-7) não alterou a resposta hipotensora da ACh em Wistar ou SHR. Nos corações isolados, a Ang-(1-7) também atenuou a redução na pressão intraventricular sistólica, dP/dt máx e dP/dt mín evocados pela ACh. O A-779 bloqueou os efeitos da Ang-(1-7) em corações de Wistar. O A-779 ou D-PRO, per se, não modificaram os efeitos da Ang-(1-7) em corações de SHR, mas na presença do D-PRO, a Ang-(1-7) apresentou efeitos similares. O vasorrelaxamento da aorta induzido pela ACh foi atenuado pela Ang-(1-7) apenas nos SHR. Estes dados sugerem que a Ang-¬(1-¬7) modula o sistema colinérgico cardíaco de forma diferente no modelo de hipertensão primária experimental e de maneira independente de ajustes na pressão arterial.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2018-10-03T11:24:00Z No. of bitstreams: 2 Dissertação - Carolina Nobre Ribeiro Pontes - 2018.pdf: 2152959 bytes, checksum: 9a1997dd7deceede7ede68d4550b490c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-10-03T11:46:07Z (GMT) No. of bitstreams: 2 Dissertação - Carolina Nobre Ribeiro Pontes - 2018.pdf: 2152959 bytes, checksum: 9a1997dd7deceede7ede68d4550b490c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-10-03T11:46:08Z (GMT). No. of bitstreams: 2 Dissertação - Carolina Nobre Ribeiro Pontes - 2018.pdf: 2152959 bytes, checksum: 9a1997dd7deceede7ede68d4550b490c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-02Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Biológicas (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessSistema renina-angiotensinaReceptor MasReceptor MuscarínicoAcetilcolinaAtividade parassimpáticaRenin-angiotensin systemMas receptorMuscarinic receptorAcetylcholineParasympathetic activityCIENCIAS BIOLOGICAS::FISIOLOGIAInfluência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensosInfluence of Angiotensin-(1-7) in cardiac cholinergic sensitivity in normotensive and hypertensive ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3362730732320261554600600600600-387277211782737340477377082474190182232075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
dc.title.alternative.eng.fl_str_mv |
Influence of Angiotensin-(1-7) in cardiac cholinergic sensitivity in normotensive and hypertensive rats |
title |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
spellingShingle |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos Pontes, Carolina Nobre Ribeiro Sistema renina-angiotensina Receptor Mas Receptor Muscarínico Acetilcolina Atividade parassimpática Renin-angiotensin system Mas receptor Muscarinic receptor Acetylcholine Parasympathetic activity CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
title_full |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
title_fullStr |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
title_full_unstemmed |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
title_sort |
Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos |
author |
Pontes, Carolina Nobre Ribeiro |
author_facet |
Pontes, Carolina Nobre Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castro, Carlos Henrique de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6354834854727314 |
dc.contributor.advisor-co1.fl_str_mv |
Custódio, Carlos Henrique Xavier |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0207928273284808 |
dc.contributor.referee1.fl_str_mv |
Pansani, Aline Priscila |
dc.contributor.referee2.fl_str_mv |
Pedrino, Gustavo Rodrigues |
dc.contributor.referee3.fl_str_mv |
Castro, Carlos Henrique de |
dc.contributor.referee4.fl_str_mv |
Custódio, Carlos Henrique Xavier |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6231711118811807 |
dc.contributor.author.fl_str_mv |
Pontes, Carolina Nobre Ribeiro |
contributor_str_mv |
Castro, Carlos Henrique de Custódio, Carlos Henrique Xavier Pansani, Aline Priscila Pedrino, Gustavo Rodrigues Castro, Carlos Henrique de Custódio, Carlos Henrique Xavier |
dc.subject.por.fl_str_mv |
Sistema renina-angiotensina Receptor Mas Receptor Muscarínico Acetilcolina Atividade parassimpática |
topic |
Sistema renina-angiotensina Receptor Mas Receptor Muscarínico Acetilcolina Atividade parassimpática Renin-angiotensin system Mas receptor Muscarinic receptor Acetylcholine Parasympathetic activity CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Renin-angiotensin system Mas receptor Muscarinic receptor Acetylcholine Parasympathetic activity |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
Previous studies suggested that the Angiotensin-(1-7) [(Ang-(1-7)] is able to modulate the cardiac sympathetic control and beta-adrenergic sensitivity. However, whether or not Ang-(1- 7) modulates the cholinergic activity in the heart remains unknown. The aim of this study was to evaluate the influence of Ang-(1-7) upon cholinergic sensitivity of hearts from normotensive and hypertensive rats. Wistar and Spontaneously Hypertensive Rats (SHR) were anesthetized with urethane and underwent catheterization of femoral artery and left ventricle to record the arterial and intraventricular pressure, respectively. Following, a dose-response curve of acetylcholine (ACh, 10, 20, 40 and 80 ng/Kg, i.v. into femoral vein) was performed in the absence or presence of Ang-(1-7) (7 x 10-12 mol/min), Mas receptor antagonist A-779 (7 x 10-11 mol/min) or Ang-(1-7)+A-779. Isolated hearts were perfused according to the Langendorff technique. Increasing concentrations of ACh (10-7 to 10-5 mol/L) were added to the hearts in absence or presence of Ang-(1-7), (2 x 10-11 mol/L), A-779, (2 x 10-10 mol/L), Ang-(1-7)+A-779, MrgD receptor antagonist, D-PRO (2 x 10-10 mol/L) or D-PRO+Ang-(1-7). ACh-induced vasorelaxation was assessed in absence or presence of Ang-(1-7) (2 x 10-11 mol/L or 2 x 10-10 mol/L). Ang-(1-7) attenuated the effect of ACh in decreasing the intraventricular systolic, dP/dt max and dP/dt min in anesthetized Wistar and SHR. These effects were blocked by A-779. Ang-(1-7) did not change the amplitude of the hypotensive effect evoked by ACh in Wistar or SHRs. In isolated hearts, Ang-(1-7) also attenuated the reduction of the intraventricular systolic pressure, dP/dt max and dP/dt min evoked by ACh. A-779 blocked the Ang-(1-7) effects in hearts from Wistar. A-779 or D-PRO did not modify the effects of Ang-(1-7) in hearts from SHR, but in presence of D-PRO, Ang-(1-7) effects were equipotent. Ang-(1-7) attenuated the vasorelaxation induced by ACh in aorta from SHR by only in SHR group. These data suggest that Ang-(1-7) exerts differential modulation of cardiac cholinergic sensitivity during experimental primary hypertension, which is independent on blood pressure. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-03T11:46:08Z |
dc.date.issued.fl_str_mv |
2018-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PONTES, C. N. R. Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos. 2018. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2018. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/8940 |
identifier_str_mv |
PONTES, C. N. R. Influência da Angiotensina-(1-7) na sensibilidade colinérgica cardíaca de ratos normotensos e hipertensos. 2018. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2018. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/8940 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3362730732320261554 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
7737708247419018223 |
dc.relation.sponsorship.fl_str_mv |
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