Análise proteômica de paracoccidioides sp. isolado de um caso de fungemia

• Autor(a) principal: Martins, Paulo Henrique Rosa
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000061nq
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/6788
• topic: Proteoma; Fungemia; Paracoccidioides; Proteomic; Fungemia; Paracoccidioides; BIOQUIMICA::BIOLOGIA MOLECULAR

Paracoccidioides spp. are pathogens of Paracoccidioidomycosis a systemic mycosis that affects about 10 million people in endemic regions. The incidence of the disease is restricted to Latin America and most cases are found in Brazil. The disease is characterized by chronic granulomatous inflammation, and patients may present with a wide spectrum of clinical manifestations. After inhalation of conidia, the installation of fungus in the lungs, which subsequently through hematogenous route may cause an infection spreads. Hematogenous fungal infections represent a serious health problem, involving hospitalized patients with predisposing conditions that lead to a high mortality rate. Fungemia corresponds to isolation of fungi in the bloodstream and occurs mainly in immunocompromised patients. Yeasts have been increasingly present as etiological agents fungemia including Candida albicans and other species such as Candida non- albicans. In this study, Paracoccidioides spp. was isolated from a case of fungemia. So far this is the first molecular study of a case of fungemia caused by this fungus. In order to identify the molecular factors associated with this specific phenotype, a comparative proteomic analysis was performed. The samples were analyzed by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoUPLC - EPM), where the soluble proteins of fungemia were compared with strain Pb01 -like proteins. 206 proteins regulated positively and negatively regulated 183 were identified. Among the positively regulated protein, 22 % were related to cellular metabolism, 15 % related to protein synthesis and 12% energy. Of phenotypic characterization tests, which showed a better adaptation of isolated Pb9840 blood were also conducted. With this work we propose that the isolated Pb9840 developed mechanisms to better installing the bloodstream and causing the fungemia. The study of the proteomic profile of this isolate may elucidate the virulence mechanisms used by this fungus during fungemia and / or hematogenous spread.