Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9468 |
Resumo: | Arboviruses are a public health problem due to outbreaks and diseases caused in several countries around the world. They are transmitted to humans and other animals through the bite of mosquito vectors, mainly of the genus Aedes. Dengue, Zika, Chikungunya and Mayaro viruses are arboviruses that share similar clinical symptoms (dengue-like), commonly fever, rash, myalgia and arthralgia. The differential diagnosis is performed through serology and / or molecular biology techniques. In Brazil these arboviruses are endemic and have a wide geographic distribution. Due to its reemergence, several rapid diagnostic kits are available in the market, and studies are needed to prove the effectiveness of these tests. The present study aims to evaluate the efficacy of two rapid lateral flow immunochromatography diagnostic kits - Orange Life® and Bahiafarma® . Both kits are available in the market. During the period from February to April 2017, 421 samples of whole blood were collected from dengue-like patients at the Hospital das Armed Forces, Brasília-DF. The hospital performed a serological screening for the following antigens - Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM using the Orange Life® kit. After sorting, two groups were selected according to inclusion and exclusion criteria based on Serological Profiles. Group 1 was 58 non-reactive samples for Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM antigens. Group 2 was 24 reagent samples for Dengue NS1 antigen. The samples were sent to the Laboratory of Molecular Genetics and Cytogenetics of the Federal University of Goiás where they were screened using the kit recommended by the Ministry of Health-Bahiafarma® Zika Combo Anti-IgM/IgG virus. Polymerase Chain Reaction (Real-time and Conventional) techniques were used for the detection of viral RNA. The molecular results for Group 1 were 4 positive samples for Dengue, 5 for Zika, 17 for Chikungunya and 2 for Mayaro. Two positive samples for Mayaro were sequenced, observing homology with other available sequences. For Group 2, 6 samples were positive for Dengue, 5 for Zika and 3 Chikungunya. Comparing the molecular results, the serological kit of Bahiafarma® Zika Combo Anti-IgM/IgG virus presented 100% specificity with a negative predictive value of 87.8%. Orange Life® serological kits showed - Dengue NS1 42.8% and 61.5% sensitivity and specificity; Dengue IgM 93% specificity with 83.56% negative predictive value; Chikungunya IgM 72.2% specificity with 56.4% negative predictive value. The results obtained in this work demonstrate that the tests evaluated have lower sensitivity and specificity than the recommended ones when the molecular results are compared. Health professionals are advised to choose the most appropriate test according to detection windows by watching the onset days of symptoms. |
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Lacerda, Elisângela de Paula Silveirahttp://lattes.cnpq.br/9390789693192751Lacerda, Elisângela de Paula SilveiraZapata, Marco TúlioSobrinho, Carlos de Almeida Baptistahttp://lattes.cnpq.br/3924895150621159Sousa, Adriano Roberto Vieira de2019-04-11T11:19:46Z2019-02-25SOUSA, Adriano Roberto Vieira de. Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas. 2019. 115 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/9468ark:/38995/0013000009tb8Arboviruses are a public health problem due to outbreaks and diseases caused in several countries around the world. They are transmitted to humans and other animals through the bite of mosquito vectors, mainly of the genus Aedes. Dengue, Zika, Chikungunya and Mayaro viruses are arboviruses that share similar clinical symptoms (dengue-like), commonly fever, rash, myalgia and arthralgia. The differential diagnosis is performed through serology and / or molecular biology techniques. In Brazil these arboviruses are endemic and have a wide geographic distribution. Due to its reemergence, several rapid diagnostic kits are available in the market, and studies are needed to prove the effectiveness of these tests. The present study aims to evaluate the efficacy of two rapid lateral flow immunochromatography diagnostic kits - Orange Life® and Bahiafarma® . Both kits are available in the market. During the period from February to April 2017, 421 samples of whole blood were collected from dengue-like patients at the Hospital das Armed Forces, Brasília-DF. The hospital performed a serological screening for the following antigens - Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM using the Orange Life® kit. After sorting, two groups were selected according to inclusion and exclusion criteria based on Serological Profiles. Group 1 was 58 non-reactive samples for Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM antigens. Group 2 was 24 reagent samples for Dengue NS1 antigen. The samples were sent to the Laboratory of Molecular Genetics and Cytogenetics of the Federal University of Goiás where they were screened using the kit recommended by the Ministry of Health-Bahiafarma® Zika Combo Anti-IgM/IgG virus. Polymerase Chain Reaction (Real-time and Conventional) techniques were used for the detection of viral RNA. The molecular results for Group 1 were 4 positive samples for Dengue, 5 for Zika, 17 for Chikungunya and 2 for Mayaro. Two positive samples for Mayaro were sequenced, observing homology with other available sequences. For Group 2, 6 samples were positive for Dengue, 5 for Zika and 3 Chikungunya. Comparing the molecular results, the serological kit of Bahiafarma® Zika Combo Anti-IgM/IgG virus presented 100% specificity with a negative predictive value of 87.8%. Orange Life® serological kits showed - Dengue NS1 42.8% and 61.5% sensitivity and specificity; Dengue IgM 93% specificity with 83.56% negative predictive value; Chikungunya IgM 72.2% specificity with 56.4% negative predictive value. The results obtained in this work demonstrate that the tests evaluated have lower sensitivity and specificity than the recommended ones when the molecular results are compared. Health professionals are advised to choose the most appropriate test according to detection windows by watching the onset days of symptoms.As arboviroses são um problema de saúde pública devido aos surtos e doenças causadas em vários países do mundo. São transmitidas para humanos e outros animais por meio da picada de mosquitos vetores, principalmente do gênero Aedes . O vírus da Dengue, Zika, Chikungunya e Mayaro são arboviroses que compartilham de sintomas clínicos semelhantes (dengue símile), comumente, febre, exantema, mialgia e artralgia. O diagnóstico diferencial é realizado através de técnicas de sorologia e/ou biologia molecular. No Brasil estas arboviroses são endêmicas e apresentam ampla distribuição geográfica. Devido à sua reemergência, vários kits de diagnóstico rápido estão disponíveis no mercado, sendo necessário estudos que comprovem a real eficácia destes testes. O presente trabalho tem como escopo avaliar a eficácia de dois kits de diagnósticos rápidos de imunocromatografia de fluxo lateral - Orange Life ® e Bahiafarma ®. Ambos os kits estão disponíveis no mercado. Durante o período de fevereiro a abril de 2017, foram coletadas 421 amostras de sangue total de pacientes com dengue símile, atendidos no Hospital das Forças Armadas, Brasília - DF. O Hospital realizou uma triagem sorológica para os seguintes antígenos - Dengue NS1 e anti - IgM/IgG; Chikungunya IgM, utilizando o kit da Orange Life ® . Após a triagem foram selecionados dois grupos, de acordo com os critérios de inclusão e exclusão baseados n o s Perfis Sorológico s . Grupo 1: 58 amostras não reagentes pa ra os antígenos Dengue NS1, Anti - IgM/ IgG e Chikungunya IgM. Grupo 2: 24 amostras reagentes para o antígeno Dengue NS1. As amostras foram encaminhadas ao Laboratório de Genética Molecular e Citogenética da Universidade Federal de Goiás, onde foram triadas utilizando o kit recomendado pelo Ministério da Saúde - Bahiafarma ® Zika vírus Combo Anti - IgM/IgG. Para a detecção do RNA viral, foi utilizado as técnicas da Reação em Cadeia da Polimerase (em Tempo Real e Convencional). Os resultados moleculares para o Grupo 1 foram 4 amostras positivas para Dengue, 5 para Zika, 17 para Chikungunya e 2 para Mayaro. As duas amostras positivas de Mayaro foram sequenciadas, observando homologia com outras sequencias disponíveis. Para o Grupo 2 foram 6 amostras positivas para Dengue, 5 para Zika e 3 Chikungunya. Comparando com os resultados moleculares, o kit sorológico da Bahiafarma ® Zika vírus Combo IgM/IgG apresentou 100% de especificidade com um valor preditivo negativo de 87,8%. Os kits sorológicos da Orange Life ® apresentaram - Dengue NS1 42,8% e 61,5% de sensibilidade e especificidade; Dengue IgM 93% de especificidade com 83,56% de valor preditivo negativo; Chikungunya IgM 72,2% de especificidade com 56,4% de valor preditivo negativo. Os resultados obtidos neste trabalho demonstram que os testes avaliados apresentam sensibilidade e especificidade menores do que o recomendado, quando comparados com os resultado s moleculares. É recomendado aos profissionais de saúde que se atentem para os dias de início dos sintomas para escolher o teste mais adequado, de acordo com as janelas de detecção.Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2019-04-10T15:17:55Z No. of bitstreams: 2 Dissertação - Adriano Roberto Vieira de Sousa - 2019.pdf: 4590497 bytes, checksum: ab7a69a95562ced84146a17ffea04bdf (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-04-11T11:19:46Z (GMT) No. of bitstreams: 2 Dissertação - Adriano Roberto Vieira de Sousa - 2019.pdf: 4590497 bytes, checksum: ab7a69a95562ced84146a17ffea04bdf (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-04-11T11:19:46Z (GMT). No. of bitstreams: 2 Dissertação - Adriano Roberto Vieira de Sousa - 2019.pdf: 4590497 bytes, checksum: ab7a69a95562ced84146a17ffea04bdf (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-02-25Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBiologia molecularInfecções por arbovirusReação em cadeia da polimeraseTestes sorológicosAedesMolecular biologyArbovirus infectionsPolymerase chain reactionSerologic testsCIENCIAS BIOLOGICAS::BIOLOGIA GERALAvaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadasSerological and molecular evaluation of dengue-like patients attended no hospital of the armed forcesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3983316729959641468600600600600-3872772117827373404-16345593859312446972075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
dc.title.alternative.eng.fl_str_mv |
Serological and molecular evaluation of dengue-like patients attended no hospital of the armed forces |
title |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
spellingShingle |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas Sousa, Adriano Roberto Vieira de Biologia molecular Infecções por arbovirus Reação em cadeia da polimerase Testes sorológicos Aedes Molecular biology Arbovirus infections Polymerase chain reaction Serologic tests CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
title_full |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
title_fullStr |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
title_full_unstemmed |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
title_sort |
Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas |
author |
Sousa, Adriano Roberto Vieira de |
author_facet |
Sousa, Adriano Roberto Vieira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lacerda, Elisângela de Paula Silveira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9390789693192751 |
dc.contributor.referee1.fl_str_mv |
Lacerda, Elisângela de Paula Silveira |
dc.contributor.referee2.fl_str_mv |
Zapata, Marco Túlio |
dc.contributor.referee3.fl_str_mv |
Sobrinho, Carlos de Almeida Baptista |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3924895150621159 |
dc.contributor.author.fl_str_mv |
Sousa, Adriano Roberto Vieira de |
contributor_str_mv |
Lacerda, Elisângela de Paula Silveira Lacerda, Elisângela de Paula Silveira Zapata, Marco Túlio Sobrinho, Carlos de Almeida Baptista |
dc.subject.por.fl_str_mv |
Biologia molecular Infecções por arbovirus Reação em cadeia da polimerase Testes sorológicos Aedes |
topic |
Biologia molecular Infecções por arbovirus Reação em cadeia da polimerase Testes sorológicos Aedes Molecular biology Arbovirus infections Polymerase chain reaction Serologic tests CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Molecular biology Arbovirus infections Polymerase chain reaction Serologic tests |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Arboviruses are a public health problem due to outbreaks and diseases caused in several countries around the world. They are transmitted to humans and other animals through the bite of mosquito vectors, mainly of the genus Aedes. Dengue, Zika, Chikungunya and Mayaro viruses are arboviruses that share similar clinical symptoms (dengue-like), commonly fever, rash, myalgia and arthralgia. The differential diagnosis is performed through serology and / or molecular biology techniques. In Brazil these arboviruses are endemic and have a wide geographic distribution. Due to its reemergence, several rapid diagnostic kits are available in the market, and studies are needed to prove the effectiveness of these tests. The present study aims to evaluate the efficacy of two rapid lateral flow immunochromatography diagnostic kits - Orange Life® and Bahiafarma® . Both kits are available in the market. During the period from February to April 2017, 421 samples of whole blood were collected from dengue-like patients at the Hospital das Armed Forces, Brasília-DF. The hospital performed a serological screening for the following antigens - Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM using the Orange Life® kit. After sorting, two groups were selected according to inclusion and exclusion criteria based on Serological Profiles. Group 1 was 58 non-reactive samples for Dengue NS1, Dengue Anti-IgM/IgG and Chikungunya IgM antigens. Group 2 was 24 reagent samples for Dengue NS1 antigen. The samples were sent to the Laboratory of Molecular Genetics and Cytogenetics of the Federal University of Goiás where they were screened using the kit recommended by the Ministry of Health-Bahiafarma® Zika Combo Anti-IgM/IgG virus. Polymerase Chain Reaction (Real-time and Conventional) techniques were used for the detection of viral RNA. The molecular results for Group 1 were 4 positive samples for Dengue, 5 for Zika, 17 for Chikungunya and 2 for Mayaro. Two positive samples for Mayaro were sequenced, observing homology with other available sequences. For Group 2, 6 samples were positive for Dengue, 5 for Zika and 3 Chikungunya. Comparing the molecular results, the serological kit of Bahiafarma® Zika Combo Anti-IgM/IgG virus presented 100% specificity with a negative predictive value of 87.8%. Orange Life® serological kits showed - Dengue NS1 42.8% and 61.5% sensitivity and specificity; Dengue IgM 93% specificity with 83.56% negative predictive value; Chikungunya IgM 72.2% specificity with 56.4% negative predictive value. The results obtained in this work demonstrate that the tests evaluated have lower sensitivity and specificity than the recommended ones when the molecular results are compared. Health professionals are advised to choose the most appropriate test according to detection windows by watching the onset days of symptoms. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-04-11T11:19:46Z |
dc.date.issued.fl_str_mv |
2019-02-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, Adriano Roberto Vieira de. Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas. 2019. 115 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9468 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000009tb8 |
identifier_str_mv |
SOUSA, Adriano Roberto Vieira de. Avaliação sorológica e molecular de pacientes com quadro clínico de dengue símile atendidos no hospital das forças armadas. 2019. 115 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019. ark:/38995/0013000009tb8 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9468 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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