PRODUÇÃO DE LACASE E BIOCONVERSÃO DE FLAVONÓIDES POR Pycnoporus sanguineus.

• Autor(a) principal: BATISTA, Francislene Lavôr
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000010bs
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/2130
• topic: bioconversão; flavonóides; lacase; Pycnoporus sanguineus; flavonoids; Pycnoporus sanguineus; laccase; CNPQ::CIENCIAS DA SAUDE::FARMACIA

The bioconversion is an area of the biotechnology that has increased in an expansive way, and it includes enzymatic reactions by microorganisms. These microorganisms that present enzymatic systems similar to those present in mammalian systems, it s the cytochrome P450 (CYP450). That s why the biotransformation an important alternative constitute as models for drug metabolism study. The Pycnoporus sanguineus is a filamentous fungi, basidiomycete of the Polyporaceae family and laccase producer (EC 1.10.3.2), an oxidoreductase enzyme. It was evaluated the influence of flavonoids naringin, naringenin, and quercetin in the growth of P. sanguineus, production of laccase in differents culture media. It was performed various conditions of reaction, such as temperature, agitation and the time of the flavonoids addition. The bioconversion processes were carried out for 24 up to 96 hours and monitored by TLC and HPLC to confirm the existence of potential metabolites. It was used purified laccase of the Pycnoporus sanguineus to evalute the participation of laccase in metabolites of naringin production. Pycnoporus sanguineus developed in differents culture media tested. Naringenin and naringin presented the capability to induce the laccase production by P. sanguineus, whereas quercetin and rutin inhibited the laccase production. The incubation using PDSM did not producted laccase. It was detected several of metabolites, whereas the incubations using quercetin acquired fourteen differents metabolites and the incubations using naringenin producted nine metabolites and naringin producted eight metabolites. In biocatalytic assay under 28ºC and 150rpm using naringin it was not detected the presence of metabolites