Efeitos da administração tópica da formulação de nanoesfera do malato de sunitinibe na inibição de neovascularização corneana induzida em coelhos

• Autor(a) principal: Linhares, Luciana Lavigne
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5840
• topic: Sunitinibe; Neovascularização corneana; Nanoesfera; Nanotecnologia; Angiogênese; Sunitinib; Corneal neovascularization; Nanosphere; Nanotechnology; Angiogenesis; CIENCIAS DA SAUDE::MEDICINA

The aim of this study was to compare the sunitinib loaded nanospheres with its free form, in the inhibition of induced corneal neovascularization, in albino rabbits, identifying the degree of inhibition of this neovascularization by measuring the area of corneal neovascularization. The eyes of eleven animals were divided into two groups. Group A (right eye of each rabbit) - sunitinib loaded nanospheres and Group B (left eye of each rabbit) - sunitinib solution. Both groups were submited to corneal alkaline burn by sodium hydroxide 1 mol/L. 12 hours after the corneal burn procedure, the group A received topical nanospheres loaded with sunitinib 0.5 mg/mL (Axon Medchem BV, Groningen, Holland), and the group B received 0.5 mg/mL of the sunitinib solution (Axon Medchem BV, Groningen, Holland). Treatment was initiated 12 hours after the surgical and was administered topically 2 times a day during 14 days. After 14 days of treatment all the rabbits were submitted to the examination of the anterior segment slit lamp examination and were photographed using the iSight camera of 8 megapixels with pixels of 1.5 μ. After euthanasia, the eyes were enucleated and sent for histopathological analysis. Neovascularization area in the upper cornea was measured in groups A and B. There was no statistically significant difference between the corneal neovascularization area in each group (p = 0.949). No changes were observed in ophthalmological clinical examination compatible with toxicity to the topical use of sunitinib loaded nanospheres and its free form. No histopathologic diferences were observed in both groups. We conclude that both the free form of sunitinib and sunitinib loaded nanospheres show no difference in inhibiting corneal neovascularization.