Detecção molecular e monitoramento sazonal de adenovírus em águas fluviais no município de Goiânia, Goiás-Brasil: correlação com parâmetros físico-químicos, bacteriológicos e Metanálise avaliativa de metodologias

• Autor(a) principal: SILVA, Hugo Delleon da
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000018dq
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/1700
• topic: adenovirus; água não tratada; Nested-PCR; Metanálise; adenovirus; untreated water; PCR; meta-analysis; CNPQ::CIENCIAS DA SAUDE

Although water is of vital importance for living beings, due to antropic action it becomes a way of dissemination of several microorganisms, which reach aquatic environments through the faeces of man and other animals and can cause several illnesses, especially for immunocompromised individuals. During routine environmental monitoring, coliform bacteria are normally used as a microbiological parameter of water quality, which does not evidence its contamination by viruses. Several researchers have proposed the detection of adenovirus (AdVs) by PCR as a molecular index to monitor other enteric viruses. AdVs are among the most persistent and ubiquitous enteric viruses present in water and associated with a variety of clinical manifestations. This study aimed to evaluate the quality of water collected from lakes and rivers in Goiânia as to the occurrence of AdVs. Water samples were collected monthly, from December 2007 to November 2008, at five different points in Goiânia (lakes of Bosque dos Buritis and Vaca Brava park, João Leite and Meia Ponte rivers downstream and upstream the municipal sewage treatment plant). The analyses were carried out at the Laboratório de Diagnóstico Genético e Molecular and Laboratório de Genética Molecular e Citogenética, Universidade Federal de Goiás. All the samples were filtered in a positively-charged nylon membrane followed by molecular detection using PCR and semi-nested PCR. Also, we performed physical-chemical and bacteriological tests to correlate these results with the occurrence of AdVs. Simultaneously, the Núcleo de Pesquisas em Agentes Emergentes e Re-emergentes carried out a meta-analysis to evaluate three methods of concentration of AdVs coupled to molecular detection in samples of untreated water. Since 29 out of the 54 water samples collected were positive for AdVs (39.2%), our results suggest the use of the methodology proposed in the present study for the detection of these pathogens in water. We observed statistically significant difference between nitrites, phosphates, fixed residues, total residues and the occurrence of AdVs, whereas no correlation was observed between fecal coliforms and AdVs. Furthermore, the occurrence of AdVs in the state of Goiás shows a seasonal trend. Based on the 33 studies selected for the meta-analysis, it was possible to get to the following interpretations: the most effective method to detect AdVs in samples from rivers or lakes was ultracentrifugation combined with nested-PCR; it is advisable to use a combination of microfiltration membrane and ultrafugation with the subsequent diagnosis using qPCR to detect AdVs in samples of treated and untreated sewage. This has been the first study carried out for the detection and monitoring of AdVs in water bodies in the Midwestern Region of Brazil and the present results may be useful to propose the eco-epidemiological profile of AdVs or even the routes of some neglected diseases, which points out the need to define a virus indicator