Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9681 |
Resumo: | The current way of sugarcane cultivation leads to the reduction of biodiversity and generates a series of environmental impacts. Thinking about the environmental issue, several strategies have been developed to make this crop less harmfull to the environment and one of these strategies refers to organic farming. The microorganisms associated with organic management are essential in the transformation and release of nutrients to plants. Among the microorganisms associated with organic cultivation, plant growth promoting rhizobacteria (PGPR) can promote plant growth in processes such as biofertilization, biostimulation and biocontrol. The present study aimed to evaluate the diversity and metabolic potential of rhizospheric communities associated with sugarcane under organic management, prospect PGPR associated with sugarcane and evaluate in vitro their potential to promote plant growth. Samples of sugarcane rhizosphere, variety CTC4, cultivated under organic and traditional management, had their DNA extracted and sequenced. The sequences obtained were analyzed with the mothur software and the alpha diversity was accessed with the Shannon and Chao1 metrics. The beta diversity was verified with the Bray-Curtis index and with principal coordinates analysis (PCoA). The functional potential of communities was predicted with the PICRUSt software. For microbial prospecting, rhizospheric microorganisms of the varieties IAC 911099 and CTC4, cultivated under organic management, were isolated. The diazotrophic rhizobacteria were isolated in NFB and Burk medium, and the actinobacteria in ISP and SCA medium. The isolated microorganisms were screened in vitro for plant growth promotion traids such as indole-acetic acid (IAA), phosphate solubilization, nitrogen fixation, ammonia production, hydrogen cyanide (HCN) production, cellulases production, chitinase production and for Fusarium moniliforme antagonism. The alpha diversity did not change due to management (p> 0.05), however, differences in the beta diversity were observed between organic and conventional samples. In relation to functional analysis, in the two places analyzed, the genes related to nitrogen fixation were more expressed in farms under organic cultivation. Of the microorganisms prospected, 81 were fast-growing diazotrophic and 21 actinobacteria. These microorganisms were distributed in 14 genera. Of these, 45.6% of the fast-growing diazotrophic and 29% of the actinobacteria were able to fix nitrogen. In addition, were found bacteria capable of producing IAA, solubilizing phosphate, producing ammonia, cellulases and chitinases. Members of Burkholderia and Streptomyces were able to inhibit the growth of phytopathogenic fungus F. moniliforme. The prospected microorganisms present potential as inoculants in the biofertilization, biostimulation and biocontrol processes and it is suggested the use of bacteria of the genus Burkholderia and the genus Streptomyces for their abilities to promote biocontrol, together with bacteria of the genus Sphingobium and Rhizobium that have abilities to promote plant growth through nitrogen fixation, phosphate solubilization and IAA production. |
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Vieira , José Daniel Gonçalveshttp://lattes.cnpq.br/1742731776579730Vieira, José Daniel GonçalvesCarneiro, Lilian CarlaSilva, Daniela de Melo eFormiga, Klebber Teodomiro MartinsCarrim, Aysha Jussara Ivonildehttp://lattes.cnpq.br/3529171839785992Rodrigues, Ariana Alves2019-06-07T10:13:39Z2018-03-29RODRIGUES, Ariana Alves. Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia. 2018. 184 f. Tese (Doutorado em Ciências Ambientais) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/9681ark:/38995/0013000001kpzThe current way of sugarcane cultivation leads to the reduction of biodiversity and generates a series of environmental impacts. Thinking about the environmental issue, several strategies have been developed to make this crop less harmfull to the environment and one of these strategies refers to organic farming. The microorganisms associated with organic management are essential in the transformation and release of nutrients to plants. Among the microorganisms associated with organic cultivation, plant growth promoting rhizobacteria (PGPR) can promote plant growth in processes such as biofertilization, biostimulation and biocontrol. The present study aimed to evaluate the diversity and metabolic potential of rhizospheric communities associated with sugarcane under organic management, prospect PGPR associated with sugarcane and evaluate in vitro their potential to promote plant growth. Samples of sugarcane rhizosphere, variety CTC4, cultivated under organic and traditional management, had their DNA extracted and sequenced. The sequences obtained were analyzed with the mothur software and the alpha diversity was accessed with the Shannon and Chao1 metrics. The beta diversity was verified with the Bray-Curtis index and with principal coordinates analysis (PCoA). The functional potential of communities was predicted with the PICRUSt software. For microbial prospecting, rhizospheric microorganisms of the varieties IAC 911099 and CTC4, cultivated under organic management, were isolated. The diazotrophic rhizobacteria were isolated in NFB and Burk medium, and the actinobacteria in ISP and SCA medium. The isolated microorganisms were screened in vitro for plant growth promotion traids such as indole-acetic acid (IAA), phosphate solubilization, nitrogen fixation, ammonia production, hydrogen cyanide (HCN) production, cellulases production, chitinase production and for Fusarium moniliforme antagonism. The alpha diversity did not change due to management (p> 0.05), however, differences in the beta diversity were observed between organic and conventional samples. In relation to functional analysis, in the two places analyzed, the genes related to nitrogen fixation were more expressed in farms under organic cultivation. Of the microorganisms prospected, 81 were fast-growing diazotrophic and 21 actinobacteria. These microorganisms were distributed in 14 genera. Of these, 45.6% of the fast-growing diazotrophic and 29% of the actinobacteria were able to fix nitrogen. In addition, were found bacteria capable of producing IAA, solubilizing phosphate, producing ammonia, cellulases and chitinases. Members of Burkholderia and Streptomyces were able to inhibit the growth of phytopathogenic fungus F. moniliforme. The prospected microorganisms present potential as inoculants in the biofertilization, biostimulation and biocontrol processes and it is suggested the use of bacteria of the genus Burkholderia and the genus Streptomyces for their abilities to promote biocontrol, together with bacteria of the genus Sphingobium and Rhizobium that have abilities to promote plant growth through nitrogen fixation, phosphate solubilization and IAA production.A atual forma de cultivo da cana-de-açúcar leva a redução da biodiversidade e gera uma série de impactos ambientais. Pensando na questão ambiental, várias estratégias têm sido desenvolvidas para tornar esse cultivo menos impactante ao meio ambiente, e uma delas refere-se ao cultivo orgânico. Os micro-organismos associados ao manejo orgânico são essenciais na transformação e liberação de nutrientes aos vegetais. Dentre os micro-organismos associados ao cultivo orgânico, as rizobactérias promotoras do crescimento vegetal (RPCV) são capazes de promover o crescimento vegetal em processos como a biofertilização, bioestimulação e biocontrole. O presente estudo teve por objetivo avaliar a diversidade e o potencial metabólico de comunidades rizosféricas associadas à cana-de-açúcar sob manejo orgânico, prospectar RPCV associadas a canaviais e avaliar in vitro seu potencial em promover o crescimento vegetal. Amostras de rizosfera de cana-de-açúcar, da variedade CTC4, cultivada sob regime orgânico e tradicional, tiveram seu DNA extraído e sequenciado. As sequências obtidas foram tratadas com o software mothur e a diversidade alfa foi acessada com as métricas Shannon e Chao1. A diversidade beta foi verificada com a métrica Bray-Curtis e a análise de coordenadas principais (PCoA). O potencial funcional das comunidades foi predito com o programa PICRUSt. Para a prospecção microbiana, foram isolados micro-organismos rizosféricos das variedades IAC 911099 e CTC4, cultivadas sob manejo orgânico. As rizobactérias diazotroficas foram isoladas em meio NFB e Burk, já as actinobactérias em meio International Streptomyces Project (ISP-2) e ágar amido caseína (AMC). Os micro-organismos isolados foram triados in vitro quanto a capacidade de produção de ácido indol ácetico (AIA), solubilização de fosfato, fixação de nitrogênio, produção de amônia, cianeto de hidrogênio (HCN), celulases, quitinase e antagonismo frente ao Fusarium moniliforme. A diversidade alfa não alterou em função do manejo (p>0.05), entretanto, puderam ser observadas diferenças na diversidade beta de comunidades associadas aos manejos convencional e orgânico de uma mesma usina. Em relação à análise funcional, nas duas usinas amostradas, os genes relacionados a fixação de nitrogênio foram mais expressos em fazendas sob cultivo orgânico. Dos micro-organismos prospectados, 81 eram diazotróficas de crescimento rápido e 21 actinobactérias. Esses micro-organismos estavam distribuidos em 14 gêneros. Destes, 45.6% das diazotróficas de crescimento rápido e 29% das actinobactérias foram capazes de fixar nitrogênio. Ademais, foram encontradas bactérias, oriundas das duas variedades e das duas usinas avaliadas, capazes de produzir AIA, solubilizar fosfato, produzir amônia, celulases e quitinases.Membros dos Burkholderia e Streptomyces foram capazes de inibir o crescimento do fungo fitopatogênico F. moniliforme. Os micro-organismos prospectados apresentam potencial como inoculantes nos processos de biofertilização, bioestimulação e biocontrole e sugere-se o uso de bactérias do gênero Burkholderia e do gênero Streptomyces, por suas habilidades em promover o biocontrole, em conjunto com bacterias do gênero Sphingobium e Rhizobium que apresentam habilidades em promover o crescimento vegetal via fixação de nitrogênio, solubilização de fosfato e produção de AIA.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2019-06-05T19:48:01Z No. of bitstreams: 2 Tese - Ariana Alves Rodrigues - 2018.pdf: 4570498 bytes, checksum: fee9b695a6a9b455e2f1f738ecb5fd30 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-06-07T10:13:39Z (GMT) No. of bitstreams: 2 Tese - Ariana Alves Rodrigues - 2018.pdf: 4570498 bytes, checksum: fee9b695a6a9b455e2f1f738ecb5fd30 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-06-07T10:13:39Z (GMT). No. of bitstreams: 2 Tese - Ariana Alves Rodrigues - 2018.pdf: 4570498 bytes, checksum: fee9b695a6a9b455e2f1f738ecb5fd30 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-29Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Ambientais (PRPG)UFGBrasilPró-Reitoria de Pós-graduação (PRPG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessRizobactériasTradicionalOrgânicoDiversidadePCVRhizobacteriaTraditionalOrganicDiversityPGPENGENHARIA TERMICA::CONTROLE AMBIENTALDiversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologiaDiversity and prospection of rhizobacteria associated with sugarcane under organic management: classical and molecular methodologies for agroecology serviceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-3687928543051349763600600600600-26453918839264606370430057436829684332075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
dc.title.alternative.eng.fl_str_mv |
Diversity and prospection of rhizobacteria associated with sugarcane under organic management: classical and molecular methodologies for agroecology service |
title |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
spellingShingle |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia Rodrigues, Ariana Alves Rizobactérias Tradicional Orgânico Diversidade PCV Rhizobacteria Traditional Organic Diversity PGP ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
title_short |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
title_full |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
title_fullStr |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
title_full_unstemmed |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
title_sort |
Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia |
author |
Rodrigues, Ariana Alves |
author_facet |
Rodrigues, Ariana Alves |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vieira , José Daniel Gonçalves |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1742731776579730 |
dc.contributor.referee1.fl_str_mv |
Vieira, José Daniel Gonçalves |
dc.contributor.referee2.fl_str_mv |
Carneiro, Lilian Carla |
dc.contributor.referee3.fl_str_mv |
Silva, Daniela de Melo e |
dc.contributor.referee4.fl_str_mv |
Formiga, Klebber Teodomiro Martins |
dc.contributor.referee5.fl_str_mv |
Carrim, Aysha Jussara Ivonilde |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3529171839785992 |
dc.contributor.author.fl_str_mv |
Rodrigues, Ariana Alves |
contributor_str_mv |
Vieira , José Daniel Gonçalves Vieira, José Daniel Gonçalves Carneiro, Lilian Carla Silva, Daniela de Melo e Formiga, Klebber Teodomiro Martins Carrim, Aysha Jussara Ivonilde |
dc.subject.por.fl_str_mv |
Rizobactérias Tradicional Orgânico Diversidade PCV |
topic |
Rizobactérias Tradicional Orgânico Diversidade PCV Rhizobacteria Traditional Organic Diversity PGP ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
dc.subject.eng.fl_str_mv |
Rhizobacteria Traditional Organic Diversity PGP |
dc.subject.cnpq.fl_str_mv |
ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
description |
The current way of sugarcane cultivation leads to the reduction of biodiversity and generates a series of environmental impacts. Thinking about the environmental issue, several strategies have been developed to make this crop less harmfull to the environment and one of these strategies refers to organic farming. The microorganisms associated with organic management are essential in the transformation and release of nutrients to plants. Among the microorganisms associated with organic cultivation, plant growth promoting rhizobacteria (PGPR) can promote plant growth in processes such as biofertilization, biostimulation and biocontrol. The present study aimed to evaluate the diversity and metabolic potential of rhizospheric communities associated with sugarcane under organic management, prospect PGPR associated with sugarcane and evaluate in vitro their potential to promote plant growth. Samples of sugarcane rhizosphere, variety CTC4, cultivated under organic and traditional management, had their DNA extracted and sequenced. The sequences obtained were analyzed with the mothur software and the alpha diversity was accessed with the Shannon and Chao1 metrics. The beta diversity was verified with the Bray-Curtis index and with principal coordinates analysis (PCoA). The functional potential of communities was predicted with the PICRUSt software. For microbial prospecting, rhizospheric microorganisms of the varieties IAC 911099 and CTC4, cultivated under organic management, were isolated. The diazotrophic rhizobacteria were isolated in NFB and Burk medium, and the actinobacteria in ISP and SCA medium. The isolated microorganisms were screened in vitro for plant growth promotion traids such as indole-acetic acid (IAA), phosphate solubilization, nitrogen fixation, ammonia production, hydrogen cyanide (HCN) production, cellulases production, chitinase production and for Fusarium moniliforme antagonism. The alpha diversity did not change due to management (p> 0.05), however, differences in the beta diversity were observed between organic and conventional samples. In relation to functional analysis, in the two places analyzed, the genes related to nitrogen fixation were more expressed in farms under organic cultivation. Of the microorganisms prospected, 81 were fast-growing diazotrophic and 21 actinobacteria. These microorganisms were distributed in 14 genera. Of these, 45.6% of the fast-growing diazotrophic and 29% of the actinobacteria were able to fix nitrogen. In addition, were found bacteria capable of producing IAA, solubilizing phosphate, producing ammonia, cellulases and chitinases. Members of Burkholderia and Streptomyces were able to inhibit the growth of phytopathogenic fungus F. moniliforme. The prospected microorganisms present potential as inoculants in the biofertilization, biostimulation and biocontrol processes and it is suggested the use of bacteria of the genus Burkholderia and the genus Streptomyces for their abilities to promote biocontrol, together with bacteria of the genus Sphingobium and Rhizobium that have abilities to promote plant growth through nitrogen fixation, phosphate solubilization and IAA production. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-03-29 |
dc.date.accessioned.fl_str_mv |
2019-06-07T10:13:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Ariana Alves. Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia. 2018. 184 f. Tese (Doutorado em Ciências Ambientais) - Universidade Federal de Goiás, Goiânia, 2018. |
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ark:/38995/0013000001kpz |
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RODRIGUES, Ariana Alves. Diversidade e prospecção de rizobactérias associadas à cana-de-açúcar sob manejo orgânico: metodologias clássicas e moleculares a serviço da agroecologia. 2018. 184 f. Tese (Doutorado em Ciências Ambientais) - Universidade Federal de Goiás, Goiânia, 2018. ark:/38995/0013000001kpz |
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Universidade Federal de Goiás |
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Universidade Federal de Goiás |
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