Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/4694 |
Resumo: | The purpose of this study was to evaluate the sedative effects, changes in physiological parameters and pharmacokinetic profile of dextro-ketamine alone or in association with midazolam, administered intravenously (cranially or caudally) or by intracoelomic route in Caiman crocodilus. Eight young specimens of spectacled caiman were anesthetized with 10 mg/kg of dextro-ketamine injected through the occipital venous sinus (DO group) or through intracoelomic injection (DI group). In addition, the same dose of dextro-ketamine was associated with 0.5 mg/kg of midazolam and administrated via the same routes (DMI or DMO groups, respectively), or within the caudal venous sinus (DMC group). Prior to drug administration (t0) and during the period of evaluation, heart rate (HR), electrocardiogram, respiratory rate (RR), body temperature (T), righting reflex, muscular relaxation of tail, limbs and head, palpebral and corneal reflexes and response to nociceptive stimuli were evaluated. Light sedation, beginning and end of deep sedation and recovery times were registered. Moreover, 24 hours before the injection of anesthetics and 15, 30, 60, 120, 240, 480, 720, 1440 e 2880 minutes after injection, 1 mL of blood was collected to delineate the pharmacokinetic profile of dextro-ketamine isolated or associated with midazolam by high performance liquid chromatography (HPLC) coupled to mass spectrometry. No animal had a negative response to nociceptive stimuli; however, the effects of DMO group were satisfactory for deep sedation. Only DI group did not presented HR alteration; in the other groups there was a significant decrease of this parameter. RR remained constant in all groups and T increased significantly only in DMC group. Pharmacokinetic analysis allowed determining the values of maximum concentration, area under the curve of plasma concentration-time, time to reach the maximum plasma drug levels, half-life, constant of elimination and clearance rate, and showed different values among groups in accordance with the route and administered drugs. We concluded the protocols cannot be used in surgical procedures. However, dextro-ketamine associated with midazolam injected in occipital venous sinus can be used for anesthesia induction and intracoelomic route can be used for application of dextro-ketamine alone for chemical restraint of Caiman crocodilus. Moreover, HPLC technique was effective in quantifying plasma levels of dextro-ketamine and midazolam of the species studied. |
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Moreno, Juan Carlos Duquehttp://lattes.cnpq.br/8509640024284103Santos, André Luiz Quagliattohttp://lattes.cnpq.br/4202407853646258Lima, Eliana Martinshttp://lattes.cnpq.br/7248774319455970Moreno, Juan Carlos DuqueVilani, Ricardo Guilherme D´Otaviano de CastroCarregaro, Adriano BentinBorges, Naida CristinaCunha, Luiz Carlos dahttp://lattes.cnpq.br/5970629029756979Hirano, Liria Queiroz Luz2015-10-21T10:20:17Z2015-02-27HIRANO, L. Q. L. Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade). 2015. 87 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/4694ark:/38995/00130000060t7The purpose of this study was to evaluate the sedative effects, changes in physiological parameters and pharmacokinetic profile of dextro-ketamine alone or in association with midazolam, administered intravenously (cranially or caudally) or by intracoelomic route in Caiman crocodilus. Eight young specimens of spectacled caiman were anesthetized with 10 mg/kg of dextro-ketamine injected through the occipital venous sinus (DO group) or through intracoelomic injection (DI group). In addition, the same dose of dextro-ketamine was associated with 0.5 mg/kg of midazolam and administrated via the same routes (DMI or DMO groups, respectively), or within the caudal venous sinus (DMC group). Prior to drug administration (t0) and during the period of evaluation, heart rate (HR), electrocardiogram, respiratory rate (RR), body temperature (T), righting reflex, muscular relaxation of tail, limbs and head, palpebral and corneal reflexes and response to nociceptive stimuli were evaluated. Light sedation, beginning and end of deep sedation and recovery times were registered. Moreover, 24 hours before the injection of anesthetics and 15, 30, 60, 120, 240, 480, 720, 1440 e 2880 minutes after injection, 1 mL of blood was collected to delineate the pharmacokinetic profile of dextro-ketamine isolated or associated with midazolam by high performance liquid chromatography (HPLC) coupled to mass spectrometry. No animal had a negative response to nociceptive stimuli; however, the effects of DMO group were satisfactory for deep sedation. Only DI group did not presented HR alteration; in the other groups there was a significant decrease of this parameter. RR remained constant in all groups and T increased significantly only in DMC group. Pharmacokinetic analysis allowed determining the values of maximum concentration, area under the curve of plasma concentration-time, time to reach the maximum plasma drug levels, half-life, constant of elimination and clearance rate, and showed different values among groups in accordance with the route and administered drugs. We concluded the protocols cannot be used in surgical procedures. However, dextro-ketamine associated with midazolam injected in occipital venous sinus can be used for anesthesia induction and intracoelomic route can be used for application of dextro-ketamine alone for chemical restraint of Caiman crocodilus. Moreover, HPLC technique was effective in quantifying plasma levels of dextro-ketamine and midazolam of the species studied.O objetivo do presente estudo foi avaliar os efeitos sedativos, alterações dos parâmetros fisiológicos e o perfil farmacocinético da dextrocetamina, isolada ou em associação ao midazolam, administrada pelas vias intravenosa (cranial ou caudal) ou intracelomática, em exemplares de Caiman crocodilus. Foram utilizados oito exemplares jovens de jacaré-tinga, anestesiados com 10 mg/kg de dextrocetamina, aplicada no seio venoso occipital (grupo DO) ou por via intracelomática (grupo DI). Adicionalmente, avaliou-se a mesma dose do dissociativo associado a 0,5 mg/kg de midazolam, ambos aplicados pelas mesmas vias dos grupos anteriores (grupo DMO e grupo DMI, respectivamente) ou pelo seio venoso caudal (grupo DMC). Antes da aplicação dos fármacos e durante o período de avaliação foram avaliados a frequência cardíaca (FC), traçado eletrocardiográfico, frequência respiratória (ƒ), temperatura corporal (TC), reação postural de endireitamento, relaxamento de cauda, membros e cabeça, reflexos palpebral e corneal e resposta ao estímulo nociceptivo. Avaliaram-se os estágios de sedação leve, de início e fim de sedação profunda e recuperação. Além disso, 24 horas antes da aplicação dos fármacos e nos tempos 15, 30, 60, 120, 240, 480, 720, 1440 e 2880 minutos após a injeção dos fármacos, foi colhido 1 mL de sangue para o delineamento do perfil farmacocinético da dextrocetamina isolada ou associada ao midazolam por meio da técnica de cromatografia líquida de alta eficiência (CLAE) acoplada a espetometria de massas. Em nenhum animal se observou ausência de resposta ao estímulo nociceptivo, entretanto, os efeitos do grupo DMO se mostraram satisfatórios para sedação profunda. Somente no grupo DI não foi observada alteração da FC, os demais grupos apresentaram diminuição significativa desse parâmetro. A ƒ se manteve constante em todos os grupos e a TC aumentou significantemente apenas no grupo DMC. A partir da análise farmacocinética conseguiu-se determinar os valores de concentração máxima, área sob a curva da concentração plasmática vs tempo, tempo para atingir a concentração máxima, meiavida, constante e taxa de depuração, com presença de diferenças entre os grupos de acordo com a via e os fármacos administrados. Concluiu-se que os protocolos não podem ser utilizados em procedimentos cirúrgicos, entretanto, a associação de dextrocetamina e midazolam no seio venoso occipital pode ser empregada para a sedação profunda e a via intracelomática pode ser utilizada para aplicação de dextrocetamina isolada na contenção química de Caiman crocodilus. Adicionalmente, a técnica de CLAE permitiu a quantificação dos níveis plasmáticos da dextrocetamina e do midazolam na espécie estudada.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2015-10-20T13:46:29Z No. of bitstreams: 2 Tese - Liria Queiroz Luz Hirano - 2015.pdf: 3409622 bytes, checksum: 058469cd4cf32b6db01eb3b924e95626 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-10-21T10:20:17Z (GMT) No. of bitstreams: 2 Tese - Liria Queiroz Luz Hirano - 2015.pdf: 3409622 bytes, checksum: 058469cd4cf32b6db01eb3b924e95626 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-10-21T10:20:17Z (GMT). No. of bitstreams: 2 Tese - Liria Queiroz Luz Hirano - 2015.pdf: 3409622 bytes, checksum: 058469cd4cf32b6db01eb3b924e95626 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-02-27Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/21225/Tese%20-%20Liria%20Queiroz%20Luz%20Hirano%20-%202015.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAnestésico dissociativoBenzodiazepínicoCrocodilianosCromatografia líquidaRépteisDissociative anestheticBenzodiazepineCrocodiliansHigh performance liquid chromatographyReptilesCLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMALContenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade)Chemical restraint and pharmacokinetic profile of dextroketamine, alone or associated with midazolam in spectacled caiman Caiman crocodilus Linnaeus (1758) (crocodylia: alligatoriedade)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis4581960685150189167600600600600-62175521142490945825710819066431151437-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
dc.title.alternative.eng.fl_str_mv |
Chemical restraint and pharmacokinetic profile of dextroketamine, alone or associated with midazolam in spectacled caiman Caiman crocodilus Linnaeus (1758) (crocodylia: alligatoriedade) |
title |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
spellingShingle |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) Hirano, Liria Queiroz Luz Anestésico dissociativo Benzodiazepínico Crocodilianos Cromatografia líquida Répteis Dissociative anesthetic Benzodiazepine Crocodilians High performance liquid chromatography Reptiles CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
title_short |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
title_full |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
title_fullStr |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
title_full_unstemmed |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
title_sort |
Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade) |
author |
Hirano, Liria Queiroz Luz |
author_facet |
Hirano, Liria Queiroz Luz |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Moreno, Juan Carlos Duque |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8509640024284103 |
dc.contributor.advisor-co1.fl_str_mv |
Santos, André Luiz Quagliatto |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4202407853646258 |
dc.contributor.advisor-co2.fl_str_mv |
Lima, Eliana Martins |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/7248774319455970 |
dc.contributor.referee1.fl_str_mv |
Moreno, Juan Carlos Duque |
dc.contributor.referee2.fl_str_mv |
Vilani, Ricardo Guilherme D´Otaviano de Castro |
dc.contributor.referee3.fl_str_mv |
Carregaro, Adriano Bentin |
dc.contributor.referee4.fl_str_mv |
Borges, Naida Cristina |
dc.contributor.referee5.fl_str_mv |
Cunha, Luiz Carlos da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5970629029756979 |
dc.contributor.author.fl_str_mv |
Hirano, Liria Queiroz Luz |
contributor_str_mv |
Moreno, Juan Carlos Duque Santos, André Luiz Quagliatto Lima, Eliana Martins Moreno, Juan Carlos Duque Vilani, Ricardo Guilherme D´Otaviano de Castro Carregaro, Adriano Bentin Borges, Naida Cristina Cunha, Luiz Carlos da |
dc.subject.por.fl_str_mv |
Anestésico dissociativo Benzodiazepínico Crocodilianos Cromatografia líquida Répteis |
topic |
Anestésico dissociativo Benzodiazepínico Crocodilianos Cromatografia líquida Répteis Dissociative anesthetic Benzodiazepine Crocodilians High performance liquid chromatography Reptiles CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
dc.subject.eng.fl_str_mv |
Dissociative anesthetic Benzodiazepine Crocodilians High performance liquid chromatography Reptiles |
dc.subject.cnpq.fl_str_mv |
CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
description |
The purpose of this study was to evaluate the sedative effects, changes in physiological parameters and pharmacokinetic profile of dextro-ketamine alone or in association with midazolam, administered intravenously (cranially or caudally) or by intracoelomic route in Caiman crocodilus. Eight young specimens of spectacled caiman were anesthetized with 10 mg/kg of dextro-ketamine injected through the occipital venous sinus (DO group) or through intracoelomic injection (DI group). In addition, the same dose of dextro-ketamine was associated with 0.5 mg/kg of midazolam and administrated via the same routes (DMI or DMO groups, respectively), or within the caudal venous sinus (DMC group). Prior to drug administration (t0) and during the period of evaluation, heart rate (HR), electrocardiogram, respiratory rate (RR), body temperature (T), righting reflex, muscular relaxation of tail, limbs and head, palpebral and corneal reflexes and response to nociceptive stimuli were evaluated. Light sedation, beginning and end of deep sedation and recovery times were registered. Moreover, 24 hours before the injection of anesthetics and 15, 30, 60, 120, 240, 480, 720, 1440 e 2880 minutes after injection, 1 mL of blood was collected to delineate the pharmacokinetic profile of dextro-ketamine isolated or associated with midazolam by high performance liquid chromatography (HPLC) coupled to mass spectrometry. No animal had a negative response to nociceptive stimuli; however, the effects of DMO group were satisfactory for deep sedation. Only DI group did not presented HR alteration; in the other groups there was a significant decrease of this parameter. RR remained constant in all groups and T increased significantly only in DMC group. Pharmacokinetic analysis allowed determining the values of maximum concentration, area under the curve of plasma concentration-time, time to reach the maximum plasma drug levels, half-life, constant of elimination and clearance rate, and showed different values among groups in accordance with the route and administered drugs. We concluded the protocols cannot be used in surgical procedures. However, dextro-ketamine associated with midazolam injected in occipital venous sinus can be used for anesthesia induction and intracoelomic route can be used for application of dextro-ketamine alone for chemical restraint of Caiman crocodilus. Moreover, HPLC technique was effective in quantifying plasma levels of dextro-ketamine and midazolam of the species studied. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-10-21T10:20:17Z |
dc.date.issued.fl_str_mv |
2015-02-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HIRANO, L. Q. L. Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade). 2015. 87 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4694 |
dc.identifier.dark.fl_str_mv |
ark:/38995/00130000060t7 |
identifier_str_mv |
HIRANO, L. Q. L. Contenção química e perfil farmacocinético da dextrocetamina, isolada em associação ao midazolam em jacaré-tinga Caiman crocodilus Linnaeus (1758) (Crocodylia: Alligatoridade). 2015. 87 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2015. ark:/38995/00130000060t7 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4694 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
5710819066431151437 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
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Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
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tasesdissertacoes.bc@ufg.br |
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1811721409869643776 |