Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/1292 |
Resumo: | Current inorganic drugs such cisplatin and related compounds widely used in the treatment cancer, however its application is limited by its severe toxicity and drug resistence. These limitations have prompted a search for news metal-based antitumor agents. Ruthenium (III) complexes represent a new family of promising metal-based anticancer drugs. In the present study, was investigated in vitro the effects of the compound on cell viability, cintetics cell cycle phases, mechanisms of apoptosis and DNA damage on tumors cells. Results of the viability using MTT reduction test and the trypan blue exclusion assay on K-562 cells revealed that this compound significantly reduced the viability of the K-562 tumors cells (IC50 approximately 10.74 and 73.45 μM), respectively, moreover viability assays on A549 lung tumor cells showed that cis-(dichloro)tetrammineruthenium(III) induced effect moderate this cell lines (IC50 > 383 μM). Additionally was observed that this compound exhibits little cytotoxicity towards MRC-5 normal human fibroblast cells (IC50 > 383 μM) when compared to K562 tumor cell line (IC50 10.74 μM). Clonogenic Assay was performed on A549 cells, and observed that lower concentrations (0.38 and 3.8 μM) cis-(dichloro)tetrammineruthenium(III) diminished colony forming ability and highest concentrations (95 and 383 μM) no colony was observed. In cell cycle analysis on K-562 and S180 tumor cells cistetrammineruthenium( III) induced change in the distribution the cell cycle phase since that % of cells entering G1, S and G2 was decreased, correlating with increase in the proportion of cells in the sub-G1-peak (indicating apoptosis). In the analysis of damage to the DNA molecule of S180 cells using the comet assay, it was observed that the ruthenium compound induced damage to the DNA molecule to both treatments (24 and 48 h) as evidenced by an increase in damage index. In addition, cis-[RuCl2(NH3)4]Cl treatment induced apoptosis in cells K-562 as evidenced for increased DNA content in the sub-G1 peak (75.35%) and a significant increase in caspase-3, 8 and 9 activity. In tumor cells S180 the apoptosis was demonstrated for by a increase numbers of Annexin V-positive cells and fragmentation DNA. Taken together, these findings strongly demonstrate that cis-[RuCl2(NH3)4]Cl exerts antitumor activity and this activity correlates with DNA damage, process apoptotic and change cell cycle. |
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LACERDA, Elisângela de Paula Silveirahttp://lattes.cnpq.br/9390789693192751http://lattes.cnpq.br/5294531830095772LIMA, Aliny Pereira de2014-07-29T15:16:37Z2010-08-172010-01-29LIMA, Aliny Pereira de. To study the potential genotoxic, cytotoxic and antitumor compound Chloride, cis-tetraaminodiclororutênio (III) on various tumor cells. 2010. 148 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2010.http://repositorio.bc.ufg.br/tede/handle/tde/1292Current inorganic drugs such cisplatin and related compounds widely used in the treatment cancer, however its application is limited by its severe toxicity and drug resistence. These limitations have prompted a search for news metal-based antitumor agents. Ruthenium (III) complexes represent a new family of promising metal-based anticancer drugs. In the present study, was investigated in vitro the effects of the compound on cell viability, cintetics cell cycle phases, mechanisms of apoptosis and DNA damage on tumors cells. Results of the viability using MTT reduction test and the trypan blue exclusion assay on K-562 cells revealed that this compound significantly reduced the viability of the K-562 tumors cells (IC50 approximately 10.74 and 73.45 μM), respectively, moreover viability assays on A549 lung tumor cells showed that cis-(dichloro)tetrammineruthenium(III) induced effect moderate this cell lines (IC50 > 383 μM). Additionally was observed that this compound exhibits little cytotoxicity towards MRC-5 normal human fibroblast cells (IC50 > 383 μM) when compared to K562 tumor cell line (IC50 10.74 μM). Clonogenic Assay was performed on A549 cells, and observed that lower concentrations (0.38 and 3.8 μM) cis-(dichloro)tetrammineruthenium(III) diminished colony forming ability and highest concentrations (95 and 383 μM) no colony was observed. In cell cycle analysis on K-562 and S180 tumor cells cistetrammineruthenium( III) induced change in the distribution the cell cycle phase since that % of cells entering G1, S and G2 was decreased, correlating with increase in the proportion of cells in the sub-G1-peak (indicating apoptosis). In the analysis of damage to the DNA molecule of S180 cells using the comet assay, it was observed that the ruthenium compound induced damage to the DNA molecule to both treatments (24 and 48 h) as evidenced by an increase in damage index. In addition, cis-[RuCl2(NH3)4]Cl treatment induced apoptosis in cells K-562 as evidenced for increased DNA content in the sub-G1 peak (75.35%) and a significant increase in caspase-3, 8 and 9 activity. In tumor cells S180 the apoptosis was demonstrated for by a increase numbers of Annexin V-positive cells and fragmentation DNA. Taken together, these findings strongly demonstrate that cis-[RuCl2(NH3)4]Cl exerts antitumor activity and this activity correlates with DNA damage, process apoptotic and change cell cycle.Atualmente drogas inorgânicas como cisplatina e compostos relacionados são amplamente utilizados no tratamento do câncer, no entanto a aplicação destas drogas é limitada devido a severa toxicidade e resistência. Estas limitações têm promovido o desenvolvimento de novos agentes antitumorais baseados em metais que sejam mais eficazes. Dentre os vários complexos a base de metais desenvolvidos, complexos de rutênio (III) representam uma nova família de promissores agentes anticâncer. No presente estudo foi investigado in vitro o efeito do composto cis-tetraaminodiclororutênio(III) sobre a viabilidade celular, cinética das fases do ciclo celular, mecanismos de indução de apoptose e danos a molécula de DNA. Os resultados de viabilidade celular utilizando os ensaios de redução do MTT e azul de tripano sobre células leucêmicas K-562 demonstrou que o composto reduziu significativamente a viabilidade celular (IC50 aproximadamente 10.74 e 73.45 μM), respectivamente, por outro lado, a viabilidade celular de células tumorais de pulmão A549 foi pouco afetada após tratamento com cis-tetraaminodiclororutênio(III) (IC50 > 383 μM). Adicionalmente, foi observado que cis-tetraaminodiclororutênio(III) exibiu uma moderada citotoxicidade sobre células normais de fibroblasto humano MRC-5 (IC50 > 383 μM) quando comparado a linhagem tumoral K-562 (10.74 e 73.45 μM) O ensaio clonogênico foi realizado em células tumorais A549, e por meio dos resultados obtidos verificou-se que em baixas concentrações do composto (0,38 e 3,8 μM) houve a diminuição na capacidade das células de formarem colônias e em concentrações elevadas 95 e 383 μM não houve a formação de nenhuma colônia. Na análise do ciclo celular de células tumorais K-562 e S-180, cistetraaminodiclororutênio( III) alterou a distribuição das fases do ciclo celular de ambas as células, visto que a porcentagem de células nas fases G1, S e G2 diminuiu, o qual correlacionou com uma aumento do número de células em sub- G1 (indicativo de apoptose). Na análise de danos a molécula de DNA de células S180 utilizando o ensaio cometa, pôde-se observar que o composto induziu danos à molécula de DNA para ambos os tratamentos (24 e 48 h) como evidenciado por um aumento do índice de dano. Além disto, o tratamento com cistetraaminodiclororutênio( III) levou a indução de apoptose nas células S180 como evidenciado pelo aumento no número de células Anexina positiva. Em células K562 a indução de apoptose após tratamento com o composto foi demonstrado pelo aumento no conteúdo de DNA em picos sub-G1 (75,35%) e um aumento na atividade de caspase 3, 8 e 9. Estes dados em conjunto demonstraram que o composto cis-tetraaminodiclororutênio(III) apresentou efeito citotóxico frente as linhagens testadas e esta atividade está correlacionada com danos à molécula de DNA, alterações nas fases do ciclo celular e indução de apoptose.Made available in DSpace on 2014-07-29T15:16:37Z (GMT). No. of bitstreams: 1 Aliny pereira.pdf: 3649782 bytes, checksum: 3c890c86afafa7051e9fcb35ec9cd8b8 (MD5) Previous issue date: 2010-01-29application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/4000/Aliny%20pereira.pdf.jpgporUniversidade Federal de GoiásMestrado em BiologiaUFGBRCiências BiolóicasAnitumoralCis-tetraaminodiclororutênio(III)CitotoxicidadeApoptoseCiclo Celular1.Câncer - tratamento 2.Antitumoral 3.Cloreto cis-tetraaminodiclororutênio (III) 4.Rutênio 5.GenotóxicoAntitumorCytotoxicityCis-tetraaminodiclororutênio(III)ApoptosisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAREstudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumoraisTo study the potential genotoxic, cytotoxic and antitumor compound Chloride, cis-tetraaminodiclororutênio (III) on various tumor cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALAliny pereira.pdfapplication/pdf3649782http://repositorio.bc.ufg.br/tede/bitstreams/63ab2c31-1440-41b7-a760-6d6231852512/download3c890c86afafa7051e9fcb35ec9cd8b8MD51TEXTAliny pereira.pdf.txtAliny pereira.pdf.txtExtracted Texttext/plain178612http://repositorio.bc.ufg.br/tede/bitstreams/cf52c22e-2435-4dce-afa6-e2756870f5f0/download8fc1189227b1f9ea0f454317446ce2b0MD52THUMBNAILAliny pereira.pdf.jpgAliny pereira.pdf.jpgGenerated Thumbnailimage/jpeg2414http://repositorio.bc.ufg.br/tede/bitstreams/a1ace3aa-5e75-4d3c-82c5-d9e03d45c133/download0e52b177f799f824a3211131e97a3158MD53tde/12922014-07-30 03:10:01.116open.accessoai:repositorio.bc.ufg.br:tde/1292http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:10:01Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
dc.title.alternative.eng.fl_str_mv |
To study the potential genotoxic, cytotoxic and antitumor compound Chloride, cis-tetraaminodiclororutênio (III) on various tumor cells |
title |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
spellingShingle |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais LIMA, Aliny Pereira de Anitumoral Cis-tetraaminodiclororutênio(III) Citotoxicidade Apoptose Ciclo Celular 1.Câncer - tratamento 2.Antitumoral 3.Cloreto cis-tetraaminodiclororutênio (III) 4.Rutênio 5.Genotóxico Antitumor Cytotoxicity Cis-tetraaminodiclororutênio(III) Apoptosis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
title_full |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
title_fullStr |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
title_full_unstemmed |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
title_sort |
Estudo do potencial genotóxico, citotóxico e antitumoral do composto Cloreto de cis-tetraaminodiclororutênio(III) sobre diferentes células tumorais |
author |
LIMA, Aliny Pereira de |
author_facet |
LIMA, Aliny Pereira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
LACERDA, Elisângela de Paula Silveira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9390789693192751 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5294531830095772 |
dc.contributor.author.fl_str_mv |
LIMA, Aliny Pereira de |
contributor_str_mv |
LACERDA, Elisângela de Paula Silveira |
dc.subject.por.fl_str_mv |
Anitumoral Cis-tetraaminodiclororutênio(III) Citotoxicidade Apoptose Ciclo Celular 1.Câncer - tratamento 2.Antitumoral 3.Cloreto cis-tetraaminodiclororutênio (III) 4.Rutênio 5.Genotóxico |
topic |
Anitumoral Cis-tetraaminodiclororutênio(III) Citotoxicidade Apoptose Ciclo Celular 1.Câncer - tratamento 2.Antitumoral 3.Cloreto cis-tetraaminodiclororutênio (III) 4.Rutênio 5.Genotóxico Antitumor Cytotoxicity Cis-tetraaminodiclororutênio(III) Apoptosis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
Antitumor Cytotoxicity Cis-tetraaminodiclororutênio(III) Apoptosis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
Current inorganic drugs such cisplatin and related compounds widely used in the treatment cancer, however its application is limited by its severe toxicity and drug resistence. These limitations have prompted a search for news metal-based antitumor agents. Ruthenium (III) complexes represent a new family of promising metal-based anticancer drugs. In the present study, was investigated in vitro the effects of the compound on cell viability, cintetics cell cycle phases, mechanisms of apoptosis and DNA damage on tumors cells. Results of the viability using MTT reduction test and the trypan blue exclusion assay on K-562 cells revealed that this compound significantly reduced the viability of the K-562 tumors cells (IC50 approximately 10.74 and 73.45 μM), respectively, moreover viability assays on A549 lung tumor cells showed that cis-(dichloro)tetrammineruthenium(III) induced effect moderate this cell lines (IC50 > 383 μM). Additionally was observed that this compound exhibits little cytotoxicity towards MRC-5 normal human fibroblast cells (IC50 > 383 μM) when compared to K562 tumor cell line (IC50 10.74 μM). Clonogenic Assay was performed on A549 cells, and observed that lower concentrations (0.38 and 3.8 μM) cis-(dichloro)tetrammineruthenium(III) diminished colony forming ability and highest concentrations (95 and 383 μM) no colony was observed. In cell cycle analysis on K-562 and S180 tumor cells cistetrammineruthenium( III) induced change in the distribution the cell cycle phase since that % of cells entering G1, S and G2 was decreased, correlating with increase in the proportion of cells in the sub-G1-peak (indicating apoptosis). In the analysis of damage to the DNA molecule of S180 cells using the comet assay, it was observed that the ruthenium compound induced damage to the DNA molecule to both treatments (24 and 48 h) as evidenced by an increase in damage index. In addition, cis-[RuCl2(NH3)4]Cl treatment induced apoptosis in cells K-562 as evidenced for increased DNA content in the sub-G1 peak (75.35%) and a significant increase in caspase-3, 8 and 9 activity. In tumor cells S180 the apoptosis was demonstrated for by a increase numbers of Annexin V-positive cells and fragmentation DNA. Taken together, these findings strongly demonstrate that cis-[RuCl2(NH3)4]Cl exerts antitumor activity and this activity correlates with DNA damage, process apoptotic and change cell cycle. |
publishDate |
2010 |
dc.date.available.fl_str_mv |
2010-08-17 |
dc.date.issued.fl_str_mv |
2010-01-29 |
dc.date.accessioned.fl_str_mv |
2014-07-29T15:16:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Aliny Pereira de. To study the potential genotoxic, cytotoxic and antitumor compound Chloride, cis-tetraaminodiclororutênio (III) on various tumor cells. 2010. 148 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/1292 |
identifier_str_mv |
LIMA, Aliny Pereira de. To study the potential genotoxic, cytotoxic and antitumor compound Chloride, cis-tetraaminodiclororutênio (III) on various tumor cells. 2010. 148 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2010. |
url |
http://repositorio.bc.ufg.br/tede/handle/tde/1292 |
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por |
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Universidade Federal de Goiás |
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Mestrado em Biologia |
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UFG |
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BR |
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Ciências Biolóicas |
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Universidade Federal de Goiás |
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