Caracterização fenotípica e molecular de Xanthomonas axonopodis pv. phaseoli e Xanthomonas fuscans subsp. fuscans procedentes de regiões produtoras de feijoeiro-comum no Brasil

• Autor(a) principal: Paiva, Bruna Alicia Rafael de
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7162
• topic: Phaseolus vulgaris; Crestamento bacteriano comum; Diversidade genética; rep- PCR; Phaseolus vulgaris; Common bacterial blight; rep-PCR; Genetic diversity; FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS

The Common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli (Xap) and Xanthomonas fuscans subsp. fuscans (Xff), is one of most important bacterial etiology diseases from the common bean plant. This pathogen causes a great loss in the production, mainly in the “water” harvest, when the environmental conditions are helpful to the development of this disease and to its dissemination. Among the strategies about integrated handling of diseases, the genetic resistance is the main measure of control and, for obtaining some cultivate with lasting resistance and of notorious specter, it is important to the know the genetic pathogen diversity. The present work has the goal of studying the diversity and the genetic structure around and inside the population of Xap and Xff using the markers rep-PCR; to connect the genetic pathogen diversity with its geographical distribution inside Brazilian producing common bean plant regions. There were obtained 42 Xap and Xff isolated and four Xanthomonas isolated not pathogens of to the bean plant, originated in the States of São Paulo, Goiás, Paraná and Rio Grande do Sul. The genetic profiles obtained by primers BOX, ERIC and REP produced 12 haplotipes matched (HC), where the HC 3, specific xff, was more frequent in PR and GO, and was not found in RS. The dendrogram produced through the analysis showed that Xap, Xff and isolated not pathogens are genetically different. In the analysis of genetic structure, the total genetic diversity (Ht), among three populations was 0,2385 revealing that there was variability from a population to the other one. Higher values of the Shannon rate (0,3648) show that Xap population is more different genetically. From a total of 51 locos, 94,12 % are polymorphic, inside Xap population they are all polymorphicand inside Xff, only 18,18 % are polymorphic. The genetic differentiation coefficient (Gst = 0,5194) revealed diversity inside and among the patogenic populations, confirming AMOVA’s result, where 51,98 % of the diversity was among the populations and 48,06 % inside them. Then, the technique helped how to find differences between Xap and Xff and measure the values of genetic diversity among and inside populations. The results of this study give us much information about the pathogens diversity, which is considered useful to identify and to characterize the resistant germoplasm in a more efficient way.