Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6637 |
Resumo: | Evidences indicate that by 2030 an increase in 40% of rice production is necessary so that it can meet the demand of the world population. The aim of this study was to quantify the expression of rice genes homologs to Arabidopsis genes previously related to yield (RUBISCO, AVP1, DA1 and TOR) by RT-qPCR analysis. The genotypes used in the study were the upland rice cultivars BRSMG Curinga and Primavera, and the old variety Douradão, which were evaluated in a yield experiment under two soil fertility levels, in a factorial scheme, in completely randomized design with two replications. Plant tissue samples were collected in vegetative and reproductive stages, which were used for total RNA isolation and subsequent cDNA syntheses. The cDNA was then subjected to RT-qPCR to evaluate the expression of four genes studied. Significant differences were observed in the expression of RUBISCO gene in leaves of upland rice plants in the vegetative stage, where there was a higher expression in high-level fertility in the soil, while in the reproductive stage, there was a higher expression in the low fertility treatment. DA1 that negatively regulates cell proliferation was less expressed in the vegetative stage in the treatment with high level of fertility, suggesting the suppression of this gene. AVP1 was more expressed in the reproductive stage, probably in order to increase the availability of P in a fundamental phase for the formation of the grain. TOR was the most expressed gene in this work, with a greater expression in adequate conditions of fertility, confirming its action under favorable conditions of cultivation. This study indicates that even two species that diverged over 120 million years have conserved productivity related metabolic routes. Thus, genes previously studied and validated in the model species Arabidopsis and that are of interest to economically important crops such as rice, may be the starting point for the development of cultivars with higher performance and better agronomic traits. |
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Brondani, Claudiohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780817T7http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4408900Z9Oliveira, João Augusto Vieira de2016-12-26T14:15:33Z2015-08-06OLIVEIRA, J. A. V. Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão. 2015. 75 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/6637Evidences indicate that by 2030 an increase in 40% of rice production is necessary so that it can meet the demand of the world population. The aim of this study was to quantify the expression of rice genes homologs to Arabidopsis genes previously related to yield (RUBISCO, AVP1, DA1 and TOR) by RT-qPCR analysis. The genotypes used in the study were the upland rice cultivars BRSMG Curinga and Primavera, and the old variety Douradão, which were evaluated in a yield experiment under two soil fertility levels, in a factorial scheme, in completely randomized design with two replications. Plant tissue samples were collected in vegetative and reproductive stages, which were used for total RNA isolation and subsequent cDNA syntheses. The cDNA was then subjected to RT-qPCR to evaluate the expression of four genes studied. Significant differences were observed in the expression of RUBISCO gene in leaves of upland rice plants in the vegetative stage, where there was a higher expression in high-level fertility in the soil, while in the reproductive stage, there was a higher expression in the low fertility treatment. DA1 that negatively regulates cell proliferation was less expressed in the vegetative stage in the treatment with high level of fertility, suggesting the suppression of this gene. AVP1 was more expressed in the reproductive stage, probably in order to increase the availability of P in a fundamental phase for the formation of the grain. TOR was the most expressed gene in this work, with a greater expression in adequate conditions of fertility, confirming its action under favorable conditions of cultivation. This study indicates that even two species that diverged over 120 million years have conserved productivity related metabolic routes. Thus, genes previously studied and validated in the model species Arabidopsis and that are of interest to economically important crops such as rice, may be the starting point for the development of cultivars with higher performance and better agronomic traits.Evidências indicam que, até 2030, será necessário um aumento em 40% da produção de arroz para que ele possa atender a demanda da população mundial. O objetivo desse estudo foi quantificar a expressão de genes de arroz homólogos a genes de Arabidopsis anteriormente relacionados à produtividade (RUBISCO, AVP1, DA1 e TOR) por meio da análise de RT-qPCR. Os genótipos usados no estudo foram as cultivares de arroz de sequeiro BRSMG Curinga e Primavera, e a cultivar antiga Douradão, as quais foram avaliadas em um experimento de rendimento sob dois níveis de fertilidade do solo, em esquema fatorial, em delineamento experimental inteiramente casualizado, com duas repetições. Amostras de tecido foliar foram coletadas nas fases vegetativa e reprodutiva, que foram utilizadas para o isolamento de RNA total e posterior síntese de cDNA. O cDNA foi, em seguida, submetido à RT-qPCR para avaliar a expressão dos quatro genes estudados. Foram observadas diferenças significativas na expressão do gene RUBISCO (Ribulose bifosfato Carboxilase/Oxigenase) em folhas de plantas de arroz de terras altas no estádio vegetativo, em que houve uma maior expressão em solo com fertilidade recomendada, enquanto que na fase reprodutiva, houve uma maior expressão no tratamento de baixa fertilidade. DA1 (Receptor de Ubiquitina), que regula negativamente a proliferação celular foi menos expresso no estádio vegetativo, no tratamento com elevado nível de fertilidade, sugerindo a supressão deste gene. AVP1(Arabidopsis Vacuolar Pirofosfatase) foi mais expresso no estádio reprodutivo, provavelmente para aumentar a disponibilidade de P em uma fase fundamental para a formação do grão. TOR (Alvo de rapamicina) foi o gene com maior nível de expressão, principalmente no solo com maior nível de fertilidade, confirmando sua ação em condições favoráveis de cultivo. Esse estudo indica que mesmo duas espécies que divergiram mais de 120 milhões anos, como é o caso de Arabidopsis e arroz, têm conservadas rotas metabólicas relacionadas à produtividade. Assim, os genes previamente estudados e validados na espécie modelo Arabidopsis e que são de interesse para as culturas economicamente importantes, como o arroz, podem ser o ponto de partida para o desenvolvimento de cultivares com maior desempenho e melhores características agronômicas.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-12-20T19:25:22Z No. of bitstreams: 2 Dissertação - João Augusto Vieira de Oliveira - 2015.pdf: 2197476 bytes, checksum: a0f442732e28ba900f7bc82946206efa (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-12-26T14:15:33Z (GMT) No. of bitstreams: 2 Dissertação - João Augusto Vieira de Oliveira - 2015.pdf: 2197476 bytes, checksum: a0f442732e28ba900f7bc82946206efa (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-12-26T14:15:33Z (GMT). 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dc.title.por.fl_str_mv |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
dc.title.alternative.eng.fl_str_mv |
Homologous genes expression in rice related to yield in arabidopsis |
title |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
spellingShingle |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão Oliveira, João Augusto Vieira de Expressão gênica Produtividade de grão Genes homólogos Gene expression Grain yield Homologous genes CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
title_full |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
title_fullStr |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
title_full_unstemmed |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
title_sort |
Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão |
author |
Oliveira, João Augusto Vieira de |
author_facet |
Oliveira, João Augusto Vieira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Brondani, Claudio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780817T7 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4408900Z9 |
dc.contributor.author.fl_str_mv |
Oliveira, João Augusto Vieira de |
contributor_str_mv |
Brondani, Claudio |
dc.subject.por.fl_str_mv |
Expressão gênica Produtividade de grão Genes homólogos |
topic |
Expressão gênica Produtividade de grão Genes homólogos Gene expression Grain yield Homologous genes CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.eng.fl_str_mv |
Gene expression Grain yield Homologous genes |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA |
description |
Evidences indicate that by 2030 an increase in 40% of rice production is necessary so that it can meet the demand of the world population. The aim of this study was to quantify the expression of rice genes homologs to Arabidopsis genes previously related to yield (RUBISCO, AVP1, DA1 and TOR) by RT-qPCR analysis. The genotypes used in the study were the upland rice cultivars BRSMG Curinga and Primavera, and the old variety Douradão, which were evaluated in a yield experiment under two soil fertility levels, in a factorial scheme, in completely randomized design with two replications. Plant tissue samples were collected in vegetative and reproductive stages, which were used for total RNA isolation and subsequent cDNA syntheses. The cDNA was then subjected to RT-qPCR to evaluate the expression of four genes studied. Significant differences were observed in the expression of RUBISCO gene in leaves of upland rice plants in the vegetative stage, where there was a higher expression in high-level fertility in the soil, while in the reproductive stage, there was a higher expression in the low fertility treatment. DA1 that negatively regulates cell proliferation was less expressed in the vegetative stage in the treatment with high level of fertility, suggesting the suppression of this gene. AVP1 was more expressed in the reproductive stage, probably in order to increase the availability of P in a fundamental phase for the formation of the grain. TOR was the most expressed gene in this work, with a greater expression in adequate conditions of fertility, confirming its action under favorable conditions of cultivation. This study indicates that even two species that diverged over 120 million years have conserved productivity related metabolic routes. Thus, genes previously studied and validated in the model species Arabidopsis and that are of interest to economically important crops such as rice, may be the starting point for the development of cultivars with higher performance and better agronomic traits. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-08-06 |
dc.date.accessioned.fl_str_mv |
2016-12-26T14:15:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
OLIVEIRA, J. A. V. Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão. 2015. 75 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6637 |
identifier_str_mv |
OLIVEIRA, J. A. V. Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão. 2015. 75 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6637 |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidade Federal de Goiás |
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