GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS

Detalhes bibliográficos
Autor(a) principal: Brunes, Tuliana Oliveira
Data de Publicação: 2007
Outros Autores: Rangel, Paulo Hideo Nakano, Brondani, Rosana Pereira , Vianello, Neto, Francisco , Moura, Neves, Péricles de Carvalho Ferreira, Brondani, Claudio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/1832
Resumo: The study aimed to evaluate the capacity of SSR markers to detect the gene flow between the red rice (RR) and the cultivated rice (CR). SSR is currently used in plant genomic analysis due to the high information content, to be co-dominant, and based on the PCR reaction. The field experiment was organized in ten concentric circles, 5 m to 50 m apart from a central red rice plant, assumed as the pollen donor. One hundred twenty rice CR plants, cv. BR-Irga 409, were planted in the intersections of the concentric circles and the twelve radii. From 51 SSR markers, four were selected due to their capacity to detect the polymorphism between RR and CR, aiming to identify RR alleles in seeds produced by BR-Irga 409 plants. The maximum distance found for gene flow between RR and CR plants was 10 m from the RR plant. In theory, at 0.1% cross pollination rate, this distance can generate 4,710 hybrids between RR and CR. In the next generation, about 3,532 plants would produce exclusively rice grains with red color. The SSR markers were able to identify the gene flow between RR and CR; therefore, they can be useful to increase the precision of cross pollination rate estimates in rice, mainly if used with other methodologies (e.g., herbicide tolerant plants). KEY WORDS: Cross pollination; microsatellite markers; Oryza sativa.
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spelling GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERSFLUXO GÊNICO ENTRE ARROZ VERMELHO E ARROZ CULTIVADO ESTIMADO POR MEIO DE MARCADORES MICROSSATÉLITESCross pollinationmicrosatellite markersOryza sativaOryza sativapolinização cruzadamarcadores microssatélitesMelhoramento de Plantas The study aimed to evaluate the capacity of SSR markers to detect the gene flow between the red rice (RR) and the cultivated rice (CR). SSR is currently used in plant genomic analysis due to the high information content, to be co-dominant, and based on the PCR reaction. The field experiment was organized in ten concentric circles, 5 m to 50 m apart from a central red rice plant, assumed as the pollen donor. One hundred twenty rice CR plants, cv. BR-Irga 409, were planted in the intersections of the concentric circles and the twelve radii. From 51 SSR markers, four were selected due to their capacity to detect the polymorphism between RR and CR, aiming to identify RR alleles in seeds produced by BR-Irga 409 plants. The maximum distance found for gene flow between RR and CR plants was 10 m from the RR plant. In theory, at 0.1% cross pollination rate, this distance can generate 4,710 hybrids between RR and CR. In the next generation, about 3,532 plants would produce exclusively rice grains with red color. The SSR markers were able to identify the gene flow between RR and CR; therefore, they can be useful to increase the precision of cross pollination rate estimates in rice, mainly if used with other methodologies (e.g., herbicide tolerant plants). KEY WORDS: Cross pollination; microsatellite markers; Oryza sativa. Este trabalho objetivou avaliar a capacidade de marcadores SSR em detectar a ocorrência de fluxo gênico entre o arroz vermelho (AV) e o arroz cultivado (AC). Marcadores SSR são utilizados em análise genômica de plantas devido ao alto conteúdo informativo, serem co-dominantes e baseados na reação de PCR. O ensaio de campo foi realizado em dez círculos concêntricos de 5 m a 50 m de distância, a partir de uma planta AV central, que foi a fonte doadora de pólen. Foram semeadas 120 plantas de arroz da cultivar BR-Irga 409, distribuídas nas interseções entre os círculos e doze raios. A partir de 51 marcadores SSR, foram selecionados quatro marcadores que detectaram polimorfismo entre o AV e AC, para identificar os alelos de AV em sementes produzidas pelas plantas da cultivar BR-Irga 409. A distância máxima encontrada entre a planta AV doadora de pólen e uma planta AC que produziu sementes híbridas (AV x AC) foi de 10 m. Teoricamente, sob 0,1% de taxa de cruzamento, esta distância de polinização pode dar origem a 4.710 sementes híbridas entre AC e AV, o que, na geração seguinte, resultará em 3.532 plantas produzindo grãos vermelhos. Marcadores SSR foram capazes de identificar fluxo gênico entre AR e AC; e, portanto, podem ser empregados em associação com outras metodologias (ex. plantas tolerantes a herbicidas) para melhorar a precisão das estimativas de polinização cruzada em arroz. PALAVRAS-CHAVE: Oryza sativa; polinização cruzada; marcadores microssatélites.Escola de Agronomia - Universidade Federal de Goiás2007-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa experimentalapplication/pdfhttps://revistas.ufg.br/pat/article/view/1832Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 37, n. 2, Apr./Jun. 2007; 86-92Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 37, n. 2, abr./jun. 2007; 86-92Pesquisa Agropecuária Tropical; v. 37, n. 2, abr./jun. 2007; 86-921983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/1832/1743Brunes, Tuliana OliveiraRangel, Paulo Hideo NakanoBrondani, Rosana Pereira , VianelloNeto, Francisco , MouraNeves, Péricles de Carvalho FerreiraBrondani, Claudioinfo:eu-repo/semantics/openAccess2020-02-27T00:01:18Zoai:ojs.revistas.ufg.br:article/1832Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:54:52.331143Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
FLUXO GÊNICO ENTRE ARROZ VERMELHO E ARROZ CULTIVADO ESTIMADO POR MEIO DE MARCADORES MICROSSATÉLITES
title GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
spellingShingle GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
Brunes, Tuliana Oliveira
Cross pollination
microsatellite markers
Oryza sativa
Oryza sativa
polinização cruzada
marcadores microssatélites
Melhoramento de Plantas
title_short GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
title_full GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
title_fullStr GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
title_full_unstemmed GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
title_sort GENE FLOW BETWEEN RED RICE AND CULTIVATED RICE ESTIMATED BY MICROSATELLITE MARKERS
author Brunes, Tuliana Oliveira
author_facet Brunes, Tuliana Oliveira
Rangel, Paulo Hideo Nakano
Brondani, Rosana Pereira , Vianello
Neto, Francisco , Moura
Neves, Péricles de Carvalho Ferreira
Brondani, Claudio
author_role author
author2 Rangel, Paulo Hideo Nakano
Brondani, Rosana Pereira , Vianello
Neto, Francisco , Moura
Neves, Péricles de Carvalho Ferreira
Brondani, Claudio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brunes, Tuliana Oliveira
Rangel, Paulo Hideo Nakano
Brondani, Rosana Pereira , Vianello
Neto, Francisco , Moura
Neves, Péricles de Carvalho Ferreira
Brondani, Claudio
dc.subject.por.fl_str_mv Cross pollination
microsatellite markers
Oryza sativa
Oryza sativa
polinização cruzada
marcadores microssatélites
Melhoramento de Plantas
topic Cross pollination
microsatellite markers
Oryza sativa
Oryza sativa
polinização cruzada
marcadores microssatélites
Melhoramento de Plantas
description The study aimed to evaluate the capacity of SSR markers to detect the gene flow between the red rice (RR) and the cultivated rice (CR). SSR is currently used in plant genomic analysis due to the high information content, to be co-dominant, and based on the PCR reaction. The field experiment was organized in ten concentric circles, 5 m to 50 m apart from a central red rice plant, assumed as the pollen donor. One hundred twenty rice CR plants, cv. BR-Irga 409, were planted in the intersections of the concentric circles and the twelve radii. From 51 SSR markers, four were selected due to their capacity to detect the polymorphism between RR and CR, aiming to identify RR alleles in seeds produced by BR-Irga 409 plants. The maximum distance found for gene flow between RR and CR plants was 10 m from the RR plant. In theory, at 0.1% cross pollination rate, this distance can generate 4,710 hybrids between RR and CR. In the next generation, about 3,532 plants would produce exclusively rice grains with red color. The SSR markers were able to identify the gene flow between RR and CR; therefore, they can be useful to increase the precision of cross pollination rate estimates in rice, mainly if used with other methodologies (e.g., herbicide tolerant plants). KEY WORDS: Cross pollination; microsatellite markers; Oryza sativa.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa experimental
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/1832
url https://revistas.ufg.br/pat/article/view/1832
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/1832/1743
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 37, n. 2, Apr./Jun. 2007; 86-92
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 37, n. 2, abr./jun. 2007; 86-92
Pesquisa Agropecuária Tropical; v. 37, n. 2, abr./jun. 2007; 86-92
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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