In vitro mycelial sensitivity of Macrophomina phaseolina to fungicides

• Autor(a) principal: Tonin, Rosane Fátima Baldiga
• Data de Publicação: 2013
• Outros Autores: Avozani, Aveline, Danelli, Anderson Luiz Durante, Reis, Erlei Melo, Zoldan, Sandra Maria, Garcés-Fiallos, Felipe Rafael
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Pesquisa Agropecuária Tropical (Online)
• Texto Completo: https://revistas.ufg.br/pat/article/view/22202
• topic: Glycine max L.; root rot; fungitoxicity; IC50.; Glycine max L.; podridão radicular; fungitoxidade; CI50.

Black root rot, caused by Macrophomina phaseolina (Tass.) Goid., is the most common root disease in soybean fields. This study aimed to determine the in vitro mycelial sensitivity, measured by the IC50 (concentration to inhibit 50% of the fungus mycelial growth) of a M. phaseolina isolate obtained from soybean, to different fungicides (thiram, iprodione, carbendazim, pyraclostrobin, fluquinconazol, tolyfluanid, metalaxyl and penflufen + trifloxystrobin), at six concentrations (0.01 mg L-1, 0.10 mg L-1, 1.00 mg L-1, 10.00 mg L-1, 20.00 mg L-1 and 40.00 mg L-1 of the active ingredient). The 0.00 mg L-1 concentration represented the control, without fungicide addition. The mycelial growth evaluation was performed with the aid of a digital pachymeter, by measuring the colonies diameter, when the fungus growth in the control treatment reached the Petri dish edge. The experimental design was completely randomized, with four replications. Concerning the fungitoxicity of active ingredients, a variation from non-toxic to highly fungitoxic was observed to the M. phaseolina isolate, with IC50 values ranging from 0.23 mg L-1 to > 40.00 mg L-1, being carbendazim the most efficient one (IC50 = 0.23 mg L-1). The fungus showed insensitivity to the active ingredients of fluquinconazole, metalaxyl, thiram and tolyfluanid.