IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/2852 |
Resumo: | Seeds of Cattleya walkeriana and Cyrtopodium palmjfrons (Orchidaceae) were aseptically transferred to culture medium Knudson, MS (Murashige& Skoog) and MS supplemented with IAA (l, 15 and 30 mg L-1) and kinetine (0.25, 5 and 10 mg L-1) for germination. They were kept at room temperature (23 +/- 2°C) under fluorescent light for 30 to 45 days. The seedlings were transferred to pots containing culture medium Knudson and incubated for four months at room temperature and transferred to fresh culture medium until the seedlings attained approximately 1.0 to 2.0 cm size for climatization. The in vitro cultivation of both plant species was considered viable. However the performance of the two orchids differed in relation to protocorms oxidation levels and in vitro growth. The results showed that less concentrated culture medium such as Knudson can be utilized , without the addition of growth regulators for the production of Cattleya walkeriana as well as Cyrtopodium palmifrons. KEY-WORDS: Orchidaceae; tissue culture; orchid. |
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IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDSPRODUÇÃO IN VITRO DE MUDAS DE Cattleya walkeriana E Cyrtopodium palmifrons A PARTIR DE SEMENTESOrchidaceaetissue cultureorchidOrchidaceaecultura de tecidosorquídea Seeds of Cattleya walkeriana and Cyrtopodium palmjfrons (Orchidaceae) were aseptically transferred to culture medium Knudson, MS (Murashige& Skoog) and MS supplemented with IAA (l, 15 and 30 mg L-1) and kinetine (0.25, 5 and 10 mg L-1) for germination. They were kept at room temperature (23 +/- 2°C) under fluorescent light for 30 to 45 days. The seedlings were transferred to pots containing culture medium Knudson and incubated for four months at room temperature and transferred to fresh culture medium until the seedlings attained approximately 1.0 to 2.0 cm size for climatization. The in vitro cultivation of both plant species was considered viable. However the performance of the two orchids differed in relation to protocorms oxidation levels and in vitro growth. The results showed that less concentrated culture medium such as Knudson can be utilized , without the addition of growth regulators for the production of Cattleya walkeriana as well as Cyrtopodium palmifrons. KEY-WORDS: Orchidaceae; tissue culture; orchid. Sementes de Cattleya walkeriana e de Cyrtopodium palmifrons (Orchidaceae) foram transferidas assepticamente para meios de cultura Knudson, MS (Murashige & Skoog) e MS suplementado com IAA (1, 15 e 30 mg L-1) e cinetina (0,25, 5 e 10 mg L-1), para germinação. Elas foram incubadas à temperatu ra de 23 +/- 2°C, sob luz fluorescente por um período de 30 a 45 dias. As plântulas obtidas foram repicadas para meio Knudson, permanecendo em incubação por quatro meses e, em seguida, transferidas para meio fresco, até atingirem tamanho para aclimatação. O cultivo in vitro de ambas as espécies foi considerado viável, havendo comportamento diferente entre as espécies, em relação à produção de protocormóides, níveis de oxidação e crescimento in vitro. Os resultados mostraram que um meio me nos concentrado como Knudson pode ser utilizado sem adição de reguladores de crescimento para produção de mudas tanto para Cattleya walkeriana quanto para Cyrtopodium palmifrons. PALAVRAS-CHAVE: Orchidaceae; cultura de tecidos; orquídea.Escola de Agronomia - Universidade Federal de Goiás2007-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/2852Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 29, n. 2, Jul./Dec. 1999; 67-71Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 29, n. 2, jul./dez. 1999; 67-71Pesquisa Agropecuária Tropical; v. 29, n. 2, jul./dez. 1999; 67-711983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/2852/2901Araújo, Leila Garcês deCarneiro, Iraídes FernandesPrabhu, Anne Sitaramainfo:eu-repo/semantics/openAccess2020-02-26T23:54:51Zoai:ojs.revistas.ufg.br:article/2852Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:55:22.269688Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS PRODUÇÃO IN VITRO DE MUDAS DE Cattleya walkeriana E Cyrtopodium palmifrons A PARTIR DE SEMENTES |
title |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
spellingShingle |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS Araújo, Leila Garcês de Orchidaceae tissue culture orchid Orchidaceae cultura de tecidos orquídea |
title_short |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
title_full |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
title_fullStr |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
title_full_unstemmed |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
title_sort |
IN VITRO PRODUCTION OF SEEDLINGS OF Cattleya walkeriana AND Cytopodium palmifrons FROM SEEDS |
author |
Araújo, Leila Garcês de |
author_facet |
Araújo, Leila Garcês de Carneiro, Iraídes Fernandes Prabhu, Anne Sitarama |
author_role |
author |
author2 |
Carneiro, Iraídes Fernandes Prabhu, Anne Sitarama |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Araújo, Leila Garcês de Carneiro, Iraídes Fernandes Prabhu, Anne Sitarama |
dc.subject.por.fl_str_mv |
Orchidaceae tissue culture orchid Orchidaceae cultura de tecidos orquídea |
topic |
Orchidaceae tissue culture orchid Orchidaceae cultura de tecidos orquídea |
description |
Seeds of Cattleya walkeriana and Cyrtopodium palmjfrons (Orchidaceae) were aseptically transferred to culture medium Knudson, MS (Murashige& Skoog) and MS supplemented with IAA (l, 15 and 30 mg L-1) and kinetine (0.25, 5 and 10 mg L-1) for germination. They were kept at room temperature (23 +/- 2°C) under fluorescent light for 30 to 45 days. The seedlings were transferred to pots containing culture medium Knudson and incubated for four months at room temperature and transferred to fresh culture medium until the seedlings attained approximately 1.0 to 2.0 cm size for climatization. The in vitro cultivation of both plant species was considered viable. However the performance of the two orchids differed in relation to protocorms oxidation levels and in vitro growth. The results showed that less concentrated culture medium such as Knudson can be utilized , without the addition of growth regulators for the production of Cattleya walkeriana as well as Cyrtopodium palmifrons. KEY-WORDS: Orchidaceae; tissue culture; orchid. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/2852 |
url |
https://revistas.ufg.br/pat/article/view/2852 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/2852/2901 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 29, n. 2, Jul./Dec. 1999; 67-71 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 29, n. 2, jul./dez. 1999; 67-71 Pesquisa Agropecuária Tropical; v. 29, n. 2, jul./dez. 1999; 67-71 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874815480299520 |