Seed viability test of orchids native to the Brazilian Savanna
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/67069 |
Resumo: | An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability. KEYWORDS: Miltonia flavescens Lindl., Schomburgkia crispa Lindl., Orchidaceae. |
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Seed viability test of orchids native to the Brazilian SavannaTeste de viabilidade de sementes de orquídeas nativas do Cerrado brasileiroAn essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability. KEYWORDS: Miltonia flavescens Lindl., Schomburgkia crispa Lindl., Orchidaceae.Um fator essencial para a formação de um banco de sementes de orquídeas nativas é a identificação da viabilidade das sementes armazenadas. Objetivou-se determinar uma metodologia para a otimização do teste de tetrazólio, na identificação da viabilidade de sementes armazenadas de duas espécies medicinais de Orchidaceae nativas do Cerrado. Sementes de Miltonia flavescens Lindl. e Schomburgkia crispa Lindl. foram submetidas a três pré-condicionamentos: sem embebição (controle), solução de sacarose ou água destilada. Em seguida foram submetidas a solução de tetrazólio, com três tipos de condicionamento: estufa, banho maria ou temperatura ambiente. As sementes de M. flavescens apresentaram germinação de 86,91 % e, quando submetidas ao pré-condicionamento com sacarose + condicionamento em estufa, mostraram maior porcentagem de sementes viáveis (62,32 %). Já para S. crispa, a germinação foi de 97,78 % e observou-se maior porcentagem de sementes viáveis no tratamento controle + temperatura ambiente (89,49 %). Esses resultados sugerem que, em Orchidaceae, protocolos específicos devem ser utilizados na condução do teste de tetrazólio e, ainda, a realização do teste de maneira amostral deve ser utilizada apenas para a indicação da viabilidade das sementes. PALAVRAS-CHAVE: Miltonia flavescens Lindl., Schomburgkia crispa Lindl., Orchidaceae.Escola de Agronomia - Universidade Federal de Goiás2021-04-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufg.br/pat/article/view/67069Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e67069Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e67069Pesquisa Agropecuária Tropical; v. 51 (2021); e670691983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/67069/36583Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessSchultz Soares, Jackeline Sorgato, José CarlosRibeiro, Luan Marlon Ramos, Jéssica Mônico Celeste 2021-04-23T11:05:16Zoai:ojs.revistas.ufg.br:article/67069Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:31.270997Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Seed viability test of orchids native to the Brazilian Savanna Teste de viabilidade de sementes de orquídeas nativas do Cerrado brasileiro |
title |
Seed viability test of orchids native to the Brazilian Savanna |
spellingShingle |
Seed viability test of orchids native to the Brazilian Savanna Schultz Soares, Jackeline |
title_short |
Seed viability test of orchids native to the Brazilian Savanna |
title_full |
Seed viability test of orchids native to the Brazilian Savanna |
title_fullStr |
Seed viability test of orchids native to the Brazilian Savanna |
title_full_unstemmed |
Seed viability test of orchids native to the Brazilian Savanna |
title_sort |
Seed viability test of orchids native to the Brazilian Savanna |
author |
Schultz Soares, Jackeline |
author_facet |
Schultz Soares, Jackeline Sorgato, José Carlos Ribeiro, Luan Marlon Ramos, Jéssica Mônico Celeste |
author_role |
author |
author2 |
Sorgato, José Carlos Ribeiro, Luan Marlon Ramos, Jéssica Mônico Celeste |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Schultz Soares, Jackeline Sorgato, José Carlos Ribeiro, Luan Marlon Ramos, Jéssica Mônico Celeste |
description |
An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability. KEYWORDS: Miltonia flavescens Lindl., Schomburgkia crispa Lindl., Orchidaceae. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/67069 |
url |
https://revistas.ufg.br/pat/article/view/67069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/67069/36583 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e67069 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e67069 Pesquisa Agropecuária Tropical; v. 51 (2021); e67069 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874821036703744 |