In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.

Detalhes bibliográficos
Autor(a) principal: Zagal Alvarado, Domitzel
Data de Publicação: 2021
Outros Autores: Lima, Andressa Priscila Piancó Santos, Santana, Raniere Ferreira de, Lima-Brito, Alone
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/69416
Resumo: Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture.
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spelling In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.Morfogênese in vitro de Physalis ixocarpa Brot ex. Horm.Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture.Physalis ixocarpa Brot. ex Horm. é considerada a espécie economicamente mais importante do gênero. A cultura de tecidos é apontada como uma estratégia para a sua propagação; no entanto, pesquisas indicam que as respostas in vitro são genótipo-dependentes. Objetivou-se avaliar a morfogênese in vitro das variedades verde e roxa de P. ixocarpa, tendo-se em vista a propagação massiva da espécie. A capacidade morfogênica dos explantes nó cotiledonar, hipocótilo e cotilédone foi avaliada em meio Murashige & Skoog suplementado com benzilaminopurina - BAP (0,00; 2,5; 5,0; 7,5; ou 10,0 µM) e ácido naftalenoacético - ANA (0,00 ou 2,5 μM), utilizando-se delineamento experimental inteiramente casualizado, em esquema fatorial 3 x 5 x 2, sendo 30 tratamentos para cada variedade. Foram analisados o número de brotos por organogênese direta e indireta e a porcentagem de explantes com calo. A expressão morfogenética in vitro de P. ixocarpa é influenciada pelo tipo de explante e pelos reguladores vegetais BAP e ANA. O explante nó cotiledonar é eficiente para a produção de brotos via organogênese direta nas duas variedades estudadas. PALAVRAS-CHAVE: Solanaceae, tomate de cáscara, propagação de plantas, cultura de tecidos.Escola de Agronomia - Universidade Federal de Goiás2021-11-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/69416Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69416Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69416Pesquisa Agropecuária Tropical; v. 51 (2021); e694161983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/69416/37470Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessZagal Alvarado, DomitzelLima, Andressa Priscila Piancó SantosSantana, Raniere Ferreira deLima-Brito, Alone2021-11-08T15:42:03Zoai:ojs.revistas.ufg.br:article/69416Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:33.255834Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
Morfogênese in vitro de Physalis ixocarpa Brot ex. Horm.
title In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
spellingShingle In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
Zagal Alvarado, Domitzel
title_short In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
title_full In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
title_fullStr In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
title_full_unstemmed In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
title_sort In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
author Zagal Alvarado, Domitzel
author_facet Zagal Alvarado, Domitzel
Lima, Andressa Priscila Piancó Santos
Santana, Raniere Ferreira de
Lima-Brito, Alone
author_role author
author2 Lima, Andressa Priscila Piancó Santos
Santana, Raniere Ferreira de
Lima-Brito, Alone
author2_role author
author
author
dc.contributor.author.fl_str_mv Zagal Alvarado, Domitzel
Lima, Andressa Priscila Piancó Santos
Santana, Raniere Ferreira de
Lima-Brito, Alone
description Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/69416
url https://revistas.ufg.br/pat/article/view/69416
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/69416/37470
dc.rights.driver.fl_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69416
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69416
Pesquisa Agropecuária Tropical; v. 51 (2021); e69416
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
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instname_str Universidade Federal de Goiás (UFG)
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reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
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