In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/69416 |
Resumo: | Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture. |
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In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm.Morfogênese in vitro de Physalis ixocarpa Brot ex. Horm.Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture.Physalis ixocarpa Brot. ex Horm. é considerada a espécie economicamente mais importante do gênero. A cultura de tecidos é apontada como uma estratégia para a sua propagação; no entanto, pesquisas indicam que as respostas in vitro são genótipo-dependentes. Objetivou-se avaliar a morfogênese in vitro das variedades verde e roxa de P. ixocarpa, tendo-se em vista a propagação massiva da espécie. A capacidade morfogênica dos explantes nó cotiledonar, hipocótilo e cotilédone foi avaliada em meio Murashige & Skoog suplementado com benzilaminopurina - BAP (0,00; 2,5; 5,0; 7,5; ou 10,0 µM) e ácido naftalenoacético - ANA (0,00 ou 2,5 μM), utilizando-se delineamento experimental inteiramente casualizado, em esquema fatorial 3 x 5 x 2, sendo 30 tratamentos para cada variedade. Foram analisados o número de brotos por organogênese direta e indireta e a porcentagem de explantes com calo. A expressão morfogenética in vitro de P. ixocarpa é influenciada pelo tipo de explante e pelos reguladores vegetais BAP e ANA. O explante nó cotiledonar é eficiente para a produção de brotos via organogênese direta nas duas variedades estudadas. PALAVRAS-CHAVE: Solanaceae, tomate de cáscara, propagação de plantas, cultura de tecidos.Escola de Agronomia - Universidade Federal de Goiás2021-11-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/69416Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69416Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69416Pesquisa Agropecuária Tropical; v. 51 (2021); e694161983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/69416/37470Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessZagal Alvarado, DomitzelLima, Andressa Priscila Piancó SantosSantana, Raniere Ferreira deLima-Brito, Alone2021-11-08T15:42:03Zoai:ojs.revistas.ufg.br:article/69416Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:33.255834Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. Morfogênese in vitro de Physalis ixocarpa Brot ex. Horm. |
title |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
spellingShingle |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. Zagal Alvarado, Domitzel |
title_short |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
title_full |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
title_fullStr |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
title_full_unstemmed |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
title_sort |
In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm. |
author |
Zagal Alvarado, Domitzel |
author_facet |
Zagal Alvarado, Domitzel Lima, Andressa Priscila Piancó Santos Santana, Raniere Ferreira de Lima-Brito, Alone |
author_role |
author |
author2 |
Lima, Andressa Priscila Piancó Santos Santana, Raniere Ferreira de Lima-Brito, Alone |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Zagal Alvarado, Domitzel Lima, Andressa Priscila Piancó Santos Santana, Raniere Ferreira de Lima-Brito, Alone |
description |
Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied. KEYWORDS: Solanaceae, husk tomato, plant propagation, tissue culture. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/69416 |
url |
https://revistas.ufg.br/pat/article/view/69416 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/69416/37470 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69416 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69416 Pesquisa Agropecuária Tropical; v. 51 (2021); e69416 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
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1799874821110104064 |