Selection of Bacillus thuringiensis strains toxic to Meloidogyne incognita

• Autor(a) principal: Santos, Jônatas Barros dos
• Data de Publicação: 2022
• Outros Autores: Silva, Alberto do Nascimento, Queiroz , Paulo Roberto Martins, Eckstein, Barbara, Monnerat, Rose Gomes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Pesquisa Agropecuária Tropical (Online)
• Texto Completo: https://revistas.ufg.br/pat/article/view/73070

The Bacillus thuringiensis bacterium has demonstrated an effective potential in the control of several agricultural pests, among them nematodes. This study aimed to standardize and establish a methodology of bioassays of B. thuringiensis and Meloidogyne incognita with the use of resorcinol, select B. thuringiensis strains toxic to M. incognita in vitro and molecularly identify the presence of the cry6 gene in B. thuringiensis strains. Second-stage juveniles were subjected to resorcinol doses, verifying that the concentration of 0.2 % did not cause mortality and provided the Cry6A toxin ingestion. Thereafter, 16 B. thuringiensis strains were tested in the presence or absence of resorcinol, resulting in mortality rates of 4-36 %. Among the B. thuringiensis strains analyzed for the presence of the cry6 gene, only one was detected (S1617). KEYWORDS: Ingestion inducer, cry6 gene, nematode.