Efficiency of a new Waitea circinata extract against rice pathogens
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/66916 |
Resumo: | Waitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast. |
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Efficiency of a new Waitea circinata extract against rice pathogensEficiência de um novo extrato de Waitea circinata contra patógenos de arrozWaitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast.Waitea circinata (Warcup & Talbot) é um fungo antagonista micorrízico de orquídea, com potencial de biocontrole contra patógenos de arroz. Objetivou-se otimizar o método de extração, obter um novo extrato e avaliar sua eficiência contra patógenos de arroz in vitro e in vivo, bem como compará-lo com outros métodos de extração e W. circinata. Os extratos foram obtidos e testados para inibição de crescimento in vitro contra os patógenos Cochliobolus miyabeanus, Monographella albescens e Sarocladium oryzae, utilizando-se os seguintes extratos: micelial, bruto, liofilizado e de massa micelial. Um ensaio adicional in vitro foi realizado com o principal patógeno do arroz (Magnaporthe oryzae), para avaliar a germinação de conídios e a formação de apressórios. Com base nessa avaliação, os extratos liofilizado e de massa micelial foram testados in vivo contra a brusone do arroz (M. oryzae) e comparados com a suspensão micelial de W. circinata, em diferentes formas de aplicação (simultânea e prévia). O extrato de massa micelial inibiu todos os patógenos, e os extratos bruto e liofilizado inibiram C. miyabeanus e M. albescens, respectivamente. O extrato de massa micelial inibiu a germinação de conídios e a formação de apressórios de M. oryzae em 80 %, e as aplicações simultâneas e prévias suprimiram a brusone em 94 %. Os resultados indicam que o novo extrato pode ser usado para controlar patógenos de arroz. PALAVRAS-CHAVE: Biocontrole, escaldadura, mancha parda, podridão da bainha, brusone.Escola de Agronomia - Universidade Federal de Goiás2021-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufg.br/pat/article/view/66916Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e66916Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e66916Pesquisa Agropecuária Tropical; v. 51 (2021); e669161983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/66916/36600Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessCarvalho, JacquelineChaibub, AmandaSousa, Kellen CristhinaBrito, DeniseFilippi, Marta CristinaKato, LucíliaAraújo, Leila Garcês2021-04-30T22:15:50Zoai:ojs.revistas.ufg.br:article/66916Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:31.036505Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Efficiency of a new Waitea circinata extract against rice pathogens Eficiência de um novo extrato de Waitea circinata contra patógenos de arroz |
title |
Efficiency of a new Waitea circinata extract against rice pathogens |
spellingShingle |
Efficiency of a new Waitea circinata extract against rice pathogens Carvalho, Jacqueline |
title_short |
Efficiency of a new Waitea circinata extract against rice pathogens |
title_full |
Efficiency of a new Waitea circinata extract against rice pathogens |
title_fullStr |
Efficiency of a new Waitea circinata extract against rice pathogens |
title_full_unstemmed |
Efficiency of a new Waitea circinata extract against rice pathogens |
title_sort |
Efficiency of a new Waitea circinata extract against rice pathogens |
author |
Carvalho, Jacqueline |
author_facet |
Carvalho, Jacqueline Chaibub, Amanda Sousa, Kellen Cristhina Brito, Denise Filippi, Marta Cristina Kato, Lucília Araújo, Leila Garcês |
author_role |
author |
author2 |
Chaibub, Amanda Sousa, Kellen Cristhina Brito, Denise Filippi, Marta Cristina Kato, Lucília Araújo, Leila Garcês |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Jacqueline Chaibub, Amanda Sousa, Kellen Cristhina Brito, Denise Filippi, Marta Cristina Kato, Lucília Araújo, Leila Garcês |
description |
Waitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/66916 |
url |
https://revistas.ufg.br/pat/article/view/66916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/66916/36600 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e66916 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e66916 Pesquisa Agropecuária Tropical; v. 51 (2021); e66916 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874821029363712 |