Efficiency of a new Waitea circinata extract against rice pathogens

Detalhes bibliográficos
Autor(a) principal: Carvalho, Jacqueline
Data de Publicação: 2021
Outros Autores: Chaibub, Amanda, Sousa, Kellen Cristhina, Brito, Denise, Filippi, Marta Cristina, Kato, Lucília, Araújo, Leila Garcês
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/66916
Resumo: Waitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast.
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spelling Efficiency of a new Waitea circinata extract against rice pathogensEficiência de um novo extrato de Waitea circinata contra patógenos de arrozWaitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast.Waitea circinata (Warcup & Talbot) é um fungo antagonista micorrízico de orquídea, com potencial de biocontrole contra patógenos de arroz. Objetivou-se otimizar o método de extração, obter um novo extrato e avaliar sua eficiência contra patógenos de arroz in vitro e in vivo, bem como compará-lo com outros métodos de extração e W. circinata. Os extratos foram obtidos e testados para inibição de crescimento in vitro contra os patógenos Cochliobolus miyabeanus, Monographella albescens e Sarocladium oryzae, utilizando-se os seguintes extratos: micelial, bruto, liofilizado e de massa micelial. Um ensaio adicional in vitro foi realizado com o principal patógeno do arroz (Magnaporthe oryzae), para avaliar a germinação de conídios e a formação de apressórios. Com base nessa avaliação, os extratos liofilizado e de massa micelial foram testados in vivo contra a brusone do arroz (M. oryzae) e comparados com a suspensão micelial de W. circinata, em diferentes formas de aplicação (simultânea e prévia). O extrato de massa micelial inibiu todos os patógenos, e os extratos bruto e liofilizado inibiram C. miyabeanus e M. albescens, respectivamente. O extrato de massa micelial inibiu a germinação de conídios e a formação de apressórios de M. oryzae em 80 %, e as aplicações simultâneas e prévias suprimiram a brusone em 94 %. Os resultados indicam que o novo extrato pode ser usado para controlar patógenos de arroz. PALAVRAS-CHAVE: Biocontrole, escaldadura, mancha parda, podridão da bainha, brusone.Escola de Agronomia - Universidade Federal de Goiás2021-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufg.br/pat/article/view/66916Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e66916Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e66916Pesquisa Agropecuária Tropical; v. 51 (2021); e669161983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/66916/36600Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessCarvalho, JacquelineChaibub, AmandaSousa, Kellen CristhinaBrito, DeniseFilippi, Marta CristinaKato, LucíliaAraújo, Leila Garcês2021-04-30T22:15:50Zoai:ojs.revistas.ufg.br:article/66916Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:31.036505Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Efficiency of a new Waitea circinata extract against rice pathogens
Eficiência de um novo extrato de Waitea circinata contra patógenos de arroz
title Efficiency of a new Waitea circinata extract against rice pathogens
spellingShingle Efficiency of a new Waitea circinata extract against rice pathogens
Carvalho, Jacqueline
title_short Efficiency of a new Waitea circinata extract against rice pathogens
title_full Efficiency of a new Waitea circinata extract against rice pathogens
title_fullStr Efficiency of a new Waitea circinata extract against rice pathogens
title_full_unstemmed Efficiency of a new Waitea circinata extract against rice pathogens
title_sort Efficiency of a new Waitea circinata extract against rice pathogens
author Carvalho, Jacqueline
author_facet Carvalho, Jacqueline
Chaibub, Amanda
Sousa, Kellen Cristhina
Brito, Denise
Filippi, Marta Cristina
Kato, Lucília
Araújo, Leila Garcês
author_role author
author2 Chaibub, Amanda
Sousa, Kellen Cristhina
Brito, Denise
Filippi, Marta Cristina
Kato, Lucília
Araújo, Leila Garcês
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Jacqueline
Chaibub, Amanda
Sousa, Kellen Cristhina
Brito, Denise
Filippi, Marta Cristina
Kato, Lucília
Araújo, Leila Garcês
description Waitea circinata (Warcup & Talbot) is an orchid antagonist mycorrhizal fungus with biocontrol potential against rice pathogens. This study aimed to optimize the extraction method, obtain a new extract and evaluate its efficiency against rice pathogens in vitro and in vivo, as well as to compare it with other extraction methods and W. circinata. The extracts were obtained and screened for in vitro growth inhibition against the pathogens Cochliobolus miyabeanus, Monographella albescens and Sarocladium oryzae, using the following extracts: mycelial, crude, lyophilized and mycelial mass. An additional in vitro assay was performed with the principal rice pathogen (Magnaporthe oryzae), in order to evaluate the conidial germination and appressorium formation. Based on this evaluation, the lyophilized and mycelial mass extracts were tested in vivo against rice blast (M. oryzae) and compared to the W. circinata mycelial suspension, in different application forms (simultaneous and previous). The mycelial mass extract inhibited all the pathogens, and the crude and lyophilized extracts inhibited C. miyabeanus and M. albescens, respectively. The mycelial mass extract inhibited the M. oryzae conidial germination and appressorium formation by 80 %, and the simultaneous and previous applications suppressed the rice blast by 94 %. These results indicate that the new extract can be used to control rice pathogens. KEYWORDS: Biocontrol, leaf scald, brown spot, sheath rot, rice blast.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/66916
url https://revistas.ufg.br/pat/article/view/66916
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/66916/36600
dc.rights.driver.fl_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e66916
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e66916
Pesquisa Agropecuária Tropical; v. 51 (2021); e66916
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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