IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/3956 |
Resumo: | This research aimed to develop a protocol for the micropropagation of Mussaenda erytrophylla cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5%) was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1). For multiplication, nodal segments from in vitro plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For rooting, nodal segments from in vitro plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts) and different concentrations of IBA (0.00 mg L-1, 0.25 mg L-1, 0.50 mg L-1, and 1.00 mg L-1). The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L-1 of NAA plus 4.0 mg L-1 of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L-1 of 2iP or 1.0 mg L-1 of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA. KEY-WORDS: Disinfection; growth regulator; callus; rooting. |
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IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea)PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)Disinfectiongrowth regulatorcallusrootingDesinfecçãoregulador de crescimentocalosenraizamento This research aimed to develop a protocol for the micropropagation of Mussaenda erytrophylla cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5%) was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1). For multiplication, nodal segments from in vitro plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For rooting, nodal segments from in vitro plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts) and different concentrations of IBA (0.00 mg L-1, 0.25 mg L-1, 0.50 mg L-1, and 1.00 mg L-1). The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L-1 of NAA plus 4.0 mg L-1 of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L-1 of 2iP or 1.0 mg L-1 of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA. KEY-WORDS: Disinfection; growth regulator; callus; rooting. O trabalho foi realizado visando a desenvolver um protocolo para micropropagação de Mussaenda erytrophylla cv. Rosea. Na assepsia de segmentos de brácteas, utilizou-se hipoclorito de cálcio ou hipoclorito de sódio (2,5%), com ou sem imersão prévia em álcool 70%. Para indução de calos, os segmentos de brácteas foram inoculados em meio MS com diferentes concentrações de BAP e ANA. Para indução de gemas, os calos foram inoculados em meio MS com diferentes concentrações das citocininas BAP, 2iP e CIN (0,0 mg L-1; 0,5 mg L-1; 1,0 mg L-1; e 2,0 mg L-1). Na multiplicação, segmentos nodais de plantas in vitro foram inoculados em meio MS com BAP, CIN e 2iP, nas concentrações de 0,0 mg L-1; 0,5 mg L-1; 1,0 mg L-1; e 2,0 mg L-1. Para o enraizamento, segmentos nodais de plantas in vitro foram inoculados no meio MS e MS/2 da formulação dos sais macronutrientes e diferentes concentrações de AIB (0,00 mg L-1; 0,25 mg L-1; 0,50 mg L-1; e 1,00 mg L-1). O melhor tratamento para a desinfestação foi aquele em que utilizou-se hipoclorito de cálcio associado à imersão em álcool. Para a indução de calos, o meio MS com 0,4 mg L-1 de ANA + 4,0 mg L-1 de BAP mostrou-se o mais efetivo. A multiplicação das gemas foi favorecida em meio MS com 0,5 mg L-1 de 2iP ou com 1,0 mg L-1 de CIN. A maior percentagem de explantes com raízes sem a formação de calos foi observada nos meios com 50% e 100% dos sais MS sem AIB. PALAVRAS-CHAVE: Desinfecção; regulador de crescimento; calos; enraizamento. Escola de Agronomia - Universidade Federal de Goiás2010-06-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa laboratorialapplication/pdfhttps://revistas.ufg.br/pat/article/view/3956Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 40, n. 2, Apr./Jun. 2010; 206-212Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 40, n. 2, abr./jun. 2010; 206-212Pesquisa Agropecuária Tropical; v. 40, n. 2, abr./jun. 2010; 206-2121983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/3956/6825Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessAlmeida, Jacqueline LeiteDiniz, Josefa Diva NogueiraOliveira, Alexandre Bosco deHernandez, Fernando Felipe Ferreyra2020-07-09T17:30:32Zoai:ojs.revistas.ufg.br:article/3956Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:55:37.087457Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
title |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
spellingShingle |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) Almeida, Jacqueline Leite Disinfection growth regulator callus rooting Desinfecção regulador de crescimento calos enraizamento |
title_short |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
title_full |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
title_fullStr |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
title_full_unstemmed |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
title_sort |
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) |
author |
Almeida, Jacqueline Leite |
author_facet |
Almeida, Jacqueline Leite Diniz, Josefa Diva Nogueira Oliveira, Alexandre Bosco de Hernandez, Fernando Felipe Ferreyra |
author_role |
author |
author2 |
Diniz, Josefa Diva Nogueira Oliveira, Alexandre Bosco de Hernandez, Fernando Felipe Ferreyra |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida, Jacqueline Leite Diniz, Josefa Diva Nogueira Oliveira, Alexandre Bosco de Hernandez, Fernando Felipe Ferreyra |
dc.subject.por.fl_str_mv |
Disinfection growth regulator callus rooting Desinfecção regulador de crescimento calos enraizamento |
topic |
Disinfection growth regulator callus rooting Desinfecção regulador de crescimento calos enraizamento |
description |
This research aimed to develop a protocol for the micropropagation of Mussaenda erytrophylla cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5%) was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1). For multiplication, nodal segments from in vitro plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For rooting, nodal segments from in vitro plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts) and different concentrations of IBA (0.00 mg L-1, 0.25 mg L-1, 0.50 mg L-1, and 1.00 mg L-1). The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L-1 of NAA plus 4.0 mg L-1 of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L-1 of 2iP or 1.0 mg L-1 of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA. KEY-WORDS: Disinfection; growth regulator; callus; rooting. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa laboratorial |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/3956 |
url |
https://revistas.ufg.br/pat/article/view/3956 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/3956/6825 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 40, n. 2, Apr./Jun. 2010; 206-212 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 40, n. 2, abr./jun. 2010; 206-212 Pesquisa Agropecuária Tropical; v. 40, n. 2, abr./jun. 2010; 206-212 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874816519438336 |