Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/23018 |
Resumo: | Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%). |
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Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicideOtimização do cultivo e padronização de pré-etapas para a obtenção de bioherbicida à base de Bipolaris euphorbiaeMass productionbioproductsconidia collecting.Produção massalbioprodutoscoleta de conídios.Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%).Disponibilizar bioprodutos para o mercado implica em buscar processos adequados para a produção massal e formulação de agentes biológicos. Neste trabalho, foi examinada a produção de Bipolaris euphorbiae em meio sólido (fermentação em substrato sólido) e em sistema bifásico (cultivo em meio líquido seguido de cultivo em meio sólido), e determinados os processos de secagem e coleta de conídios, em condições de laboratório. Investigou-se a influência do período de incubação e a quantidade de inóculo. A secagem dos conídios foi realizada por meio de estufa (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC e 60ºC) e fluxo laminar, fluxo contínuo de ar e câmara asséptica em temperatura ambiente. Os conídios secos foram obtidos por peneiramento e moagem em moinho de esfera, de martelo ou de grãos. Avaliou-se a viabilidade dos conídios e a esporulação no meio sólido e sistema bifásico. Considerando-se o período de crescimento, a melhor esporulação no meio sólido foi obtida após 10 dias de incubação, alcançando 8,3 x 107 conídios g-1 de substrato. O sistema bifásico não incrementou a esporulação de B. euphorbiae (4,5 x 107 conídios g-1 de substrato) cultivado por 14 dias, e a quantidade de inóculo líquido usado neste sistema não foi determinante para incrementar a sua produção. O fluxo contínuo de ar e o fluxo laminar mantiveram a viabilidade dos conídios (94,6% e 99,1%, respectivamente) e promoveram grande perda de umidade (62,6% e 54,0%, respectivamente). Todos os processos de moagem reduziram a germinação dos conídios (86,2%, 10,5% e 12%, respectivamente), enquanto o peneiramento proporcionou a coleta de conídios em pó com alta viabilidade (94,8%).Escola de Agronomia - Universidade Federal de Goiás2013-11-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Experimental; Métodos Quantitativosapplication/pdfhttps://revistas.ufg.br/pat/article/view/23018Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 43, n. 4, Oct./Dec. 2013; 392-399Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 43, n. 4, out./dez. 2013; 392-399Pesquisa Agropecuária Tropical; v. 43, n. 4, out./dez. 2013; 392-3991983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/23018/15550Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessMachado, Ana Carolina RibeiroMochi, Dinalva AlvesMonteiro, Antonio Carlos2020-07-10T12:54:35Zoai:ojs.revistas.ufg.br:article/23018Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:00.552218Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide Otimização do cultivo e padronização de pré-etapas para a obtenção de bioherbicida à base de Bipolaris euphorbiae |
title |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
spellingShingle |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide Machado, Ana Carolina Ribeiro Mass production bioproducts conidia collecting. Produção massal bioprodutos coleta de conídios. |
title_short |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
title_full |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
title_fullStr |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
title_full_unstemmed |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
title_sort |
Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide |
author |
Machado, Ana Carolina Ribeiro |
author_facet |
Machado, Ana Carolina Ribeiro Mochi, Dinalva Alves Monteiro, Antonio Carlos |
author_role |
author |
author2 |
Mochi, Dinalva Alves Monteiro, Antonio Carlos |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Machado, Ana Carolina Ribeiro Mochi, Dinalva Alves Monteiro, Antonio Carlos |
dc.subject.por.fl_str_mv |
Mass production bioproducts conidia collecting. Produção massal bioprodutos coleta de conídios. |
topic |
Mass production bioproducts conidia collecting. Produção massal bioprodutos coleta de conídios. |
description |
Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%). |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Experimental; Métodos Quantitativos |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/23018 |
url |
https://revistas.ufg.br/pat/article/view/23018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/23018/15550 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 43, n. 4, Oct./Dec. 2013; 392-399 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 43, n. 4, out./dez. 2013; 392-399 Pesquisa Agropecuária Tropical; v. 43, n. 4, out./dez. 2013; 392-399 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874818355494912 |