Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide

Detalhes bibliográficos
Autor(a) principal: Machado, Ana Carolina Ribeiro
Data de Publicação: 2013
Outros Autores: Mochi, Dinalva Alves, Monteiro, Antonio Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/23018
Resumo: Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%).
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spelling Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicideOtimização do cultivo e padronização de pré-etapas para a obtenção de bioherbicida à base de Bipolaris euphorbiaeMass productionbioproductsconidia collecting.Produção massalbioprodutoscoleta de conídios.Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%).Disponibilizar bioprodutos para o mercado implica em buscar processos adequados para a produção massal e formulação de agentes biológicos. Neste trabalho, foi examinada a produção de Bipolaris euphorbiae em meio sólido (fermentação em substrato sólido) e em sistema bifásico (cultivo em meio líquido seguido de cultivo em meio sólido), e determinados os processos de secagem e coleta de conídios, em condições de laboratório. Investigou-se a influência do período de incubação e a quantidade de inóculo. A secagem dos conídios foi realizada por meio de estufa (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC e 60ºC) e fluxo laminar, fluxo contínuo de ar e câmara asséptica em temperatura ambiente. Os conídios secos foram obtidos por peneiramento e moagem em moinho de esfera, de martelo ou de grãos. Avaliou-se a viabilidade dos conídios e a esporulação no meio sólido e sistema bifásico. Considerando-se o período de crescimento, a melhor esporulação no meio sólido foi obtida após 10 dias de incubação, alcançando 8,3 x 107 conídios g-1 de substrato. O sistema bifásico não incrementou a esporulação de B. euphorbiae (4,5 x 107 conídios g-1 de substrato) cultivado por 14 dias, e a quantidade de inóculo líquido usado neste sistema não foi determinante para incrementar a sua produção. O fluxo contínuo de ar e o fluxo laminar mantiveram a viabilidade dos conídios (94,6% e 99,1%, respectivamente) e promoveram grande perda de umidade (62,6% e 54,0%, respectivamente). Todos os processos de moagem reduziram a germinação dos conídios (86,2%, 10,5% e 12%, respectivamente), enquanto o peneiramento proporcionou a coleta de conídios em pó com alta viabilidade (94,8%).Escola de Agronomia - Universidade Federal de Goiás2013-11-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Experimental; Métodos Quantitativosapplication/pdfhttps://revistas.ufg.br/pat/article/view/23018Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 43, n. 4, Oct./Dec. 2013; 392-399Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 43, n. 4, out./dez. 2013; 392-399Pesquisa Agropecuária Tropical; v. 43, n. 4, out./dez. 2013; 392-3991983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/23018/15550Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessMachado, Ana Carolina RibeiroMochi, Dinalva AlvesMonteiro, Antonio Carlos2020-07-10T12:54:35Zoai:ojs.revistas.ufg.br:article/23018Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:00.552218Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
Otimização do cultivo e padronização de pré-etapas para a obtenção de bioherbicida à base de Bipolaris euphorbiae
title Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
spellingShingle Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
Machado, Ana Carolina Ribeiro
Mass production
bioproducts
conidia collecting.
Produção massal
bioprodutos
coleta de conídios.
title_short Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
title_full Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
title_fullStr Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
title_full_unstemmed Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
title_sort Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide
author Machado, Ana Carolina Ribeiro
author_facet Machado, Ana Carolina Ribeiro
Mochi, Dinalva Alves
Monteiro, Antonio Carlos
author_role author
author2 Mochi, Dinalva Alves
Monteiro, Antonio Carlos
author2_role author
author
dc.contributor.author.fl_str_mv Machado, Ana Carolina Ribeiro
Mochi, Dinalva Alves
Monteiro, Antonio Carlos
dc.subject.por.fl_str_mv Mass production
bioproducts
conidia collecting.
Produção massal
bioprodutos
coleta de conídios.
topic Mass production
bioproducts
conidia collecting.
Produção massal
bioprodutos
coleta de conídios.
description Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 107 conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 107 conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%).
publishDate 2013
dc.date.none.fl_str_mv 2013-11-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Experimental; Métodos Quantitativos
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/23018
url https://revistas.ufg.br/pat/article/view/23018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/23018/15550
dc.rights.driver.fl_str_mv Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 43, n. 4, Oct./Dec. 2013; 392-399
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 43, n. 4, out./dez. 2013; 392-399
Pesquisa Agropecuária Tropical; v. 43, n. 4, out./dez. 2013; 392-399
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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