Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid

Detalhes bibliográficos
Autor(a) principal: Camayo Mosquera, Deysi Jhoana
Data de Publicação: 2021
Outros Autores: Cayón Salinas, Daniel Gerardo, Ligarreto, Gustavo Adolfo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/68076
Resumo: Elaeis oleifera chromosomes are similar to those of E. guineensis, with close gene pools for the production of interspecific O x G hybrids. The pollen viability and germination of E. oleifera ‘Coarí’ and E. guineensis ‘La Mé’ were compared to their interspecific hybrid O x G (‘Coarí’ x ‘La Mé’). The pollen viability was determined by the acetocarmine staining method (0.5 %) and the pollen germination by in vitro incubation on agar-sucrose medium (1.2-11.0 g in 100 mL of distilled water). The pollen viability and germination of the ‘Coarí’ x ‘La Mé’ hybrid were significantly lower than those of their parents. The percentage of pollen viability by acetocarmine staining was higher than that of in vitro germination, indicating that not all pollen grains classified as viable germinated on the agar-sucrose medium. The pollen germination test is a more reliable indicator than the staining viability test, because the latter only reveals that the pollen contains the enzymes necessary to initiate germination, while the germination test determines the emission and development of the pollen tube. KEYWORDS: Plant physiology, acetocarmine, pollination.
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spelling Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybridViabilidade e germinação de pólen em Elaeis oleifera, Elaeis guineensis e seu híbrido interespecíficoElaeis oleifera chromosomes are similar to those of E. guineensis, with close gene pools for the production of interspecific O x G hybrids. The pollen viability and germination of E. oleifera ‘Coarí’ and E. guineensis ‘La Mé’ were compared to their interspecific hybrid O x G (‘Coarí’ x ‘La Mé’). The pollen viability was determined by the acetocarmine staining method (0.5 %) and the pollen germination by in vitro incubation on agar-sucrose medium (1.2-11.0 g in 100 mL of distilled water). The pollen viability and germination of the ‘Coarí’ x ‘La Mé’ hybrid were significantly lower than those of their parents. The percentage of pollen viability by acetocarmine staining was higher than that of in vitro germination, indicating that not all pollen grains classified as viable germinated on the agar-sucrose medium. The pollen germination test is a more reliable indicator than the staining viability test, because the latter only reveals that the pollen contains the enzymes necessary to initiate germination, while the germination test determines the emission and development of the pollen tube. KEYWORDS: Plant physiology, acetocarmine, pollination.Os cromossomos de Elaeis oleifera são semelhantes aos de E. guineensis, com pools genéticos próximos para a produção de híbridos interespecíficos O x G. A viabilidade e germinação do pólen de E. oleifera ‘Coarí’ e E. guineensis ‘La Mé’ foram comparadas com as de seu híbrido interespecífico O x G (‘Coarí’ x ‘La Mé’). A viabilidade do pólen foi determinada pelo método de coloração com acetocarmina (0,5 %) e a germinação por incubação in vitro, em meio ágar-sacarose (1,2-11,0 g em 100 mL de água destilada). A viabilidade e germinação de pólen do híbrido ‘Coarí’ x ‘La Mé’ foram significativamente menores do que as de seus progenitores. A porcentagem de viabilidade do pólen pela coloração com acetocarmina foi superior à germinação in vitro, indicando que nem todos os grãos de pólen classificados como viáveis ​​germinaram no meio ágar-sacarose. O teste de germinação de pólen é um indicador mais confiável que o de viabilidade por coloração, pois este apenas revela que o pólen contém as enzimas necessárias para iniciar a germinação, enquanto o teste de germinação determina a emissão e desenvolvimento do tubo polínico. PALAVRAS-CHAVE: Fisiologia vegetal, acetocarmina, polinização.Escola de Agronomia - Universidade Federal de Goiás2021-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/68076Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e68076Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e68076Pesquisa Agropecuária Tropical; v. 51 (2021); e680761983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/68076/36741Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessCamayo Mosquera, Deysi Jhoana Cayón Salinas, Daniel Gerardo Ligarreto, Gustavo Adolfo2021-06-15T17:46:40Zoai:ojs.revistas.ufg.br:article/68076Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:32.388396Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
Viabilidade e germinação de pólen em Elaeis oleifera, Elaeis guineensis e seu híbrido interespecífico
title Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
spellingShingle Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
Camayo Mosquera, Deysi Jhoana
title_short Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
title_full Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
title_fullStr Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
title_full_unstemmed Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
title_sort Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid
author Camayo Mosquera, Deysi Jhoana
author_facet Camayo Mosquera, Deysi Jhoana
Cayón Salinas, Daniel Gerardo
Ligarreto, Gustavo Adolfo
author_role author
author2 Cayón Salinas, Daniel Gerardo
Ligarreto, Gustavo Adolfo
author2_role author
author
dc.contributor.author.fl_str_mv Camayo Mosquera, Deysi Jhoana
Cayón Salinas, Daniel Gerardo
Ligarreto, Gustavo Adolfo
description Elaeis oleifera chromosomes are similar to those of E. guineensis, with close gene pools for the production of interspecific O x G hybrids. The pollen viability and germination of E. oleifera ‘Coarí’ and E. guineensis ‘La Mé’ were compared to their interspecific hybrid O x G (‘Coarí’ x ‘La Mé’). The pollen viability was determined by the acetocarmine staining method (0.5 %) and the pollen germination by in vitro incubation on agar-sucrose medium (1.2-11.0 g in 100 mL of distilled water). The pollen viability and germination of the ‘Coarí’ x ‘La Mé’ hybrid were significantly lower than those of their parents. The percentage of pollen viability by acetocarmine staining was higher than that of in vitro germination, indicating that not all pollen grains classified as viable germinated on the agar-sucrose medium. The pollen germination test is a more reliable indicator than the staining viability test, because the latter only reveals that the pollen contains the enzymes necessary to initiate germination, while the germination test determines the emission and development of the pollen tube. KEYWORDS: Plant physiology, acetocarmine, pollination.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/68076
url https://revistas.ufg.br/pat/article/view/68076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/68076/36741
dc.rights.driver.fl_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Pesquisa Agropecuária Tropical
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e68076
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e68076
Pesquisa Agropecuária Tropical; v. 51 (2021); e68076
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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