TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS

Detalhes bibliográficos
Autor(a) principal: Carvalho, Alessandra Cristina Francischini
Data de Publicação: 2015
Outros Autores: Pacheco, Maria Rita, Baraldi-Artoni, Silvana Martinez, Girardi, Annita Morais
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/18991
Resumo: The aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and were used as the control group (group 1), and ten dogs were nonsymptomatic but seropositive for toxoplasmosis (group 2).  After the microtomy, with interval of 100 micrometers (µm), the histological 5-µm-thick cuts were dyed by hematoxylin-eosin and Masson's trichrome techniques. The glass slides were analyzed under light microscope to examine the neuron morphology. The parameters considered for the morphometric analysis were area, perimeter, maximum diameter, minimum diameter and shape factor of cytoplasm and nucleus of neuron. The results were statistically analyzed by Student’s t test at 5% probability level. The morphological characteristics between the two groups were similar and according to literature. The morphometric results showed that there were changes in neurons size and structure, and increase and loss of star shape were noticed in seropositive animals. The results suggest that the neurons of these dogs, yet nonsymptomatic, can have lost their conductor function. Keywords: canine; central nervous system; histology; Toxoplasma gondii. 
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spelling TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGSTOXOPLASMOSE: AVALIAÇÃO MORFOLÓGICA E MORFOMÉTRICA DOS NEURÔNIOS DA MEDULA ESPINHAL DE CÃES SOROPOSITIVOS ASSINTOMÁTICOSCanineCentral nervous systemHistologyToxoplasma gondiiSaúde AnimalThe aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and were used as the control group (group 1), and ten dogs were nonsymptomatic but seropositive for toxoplasmosis (group 2).  After the microtomy, with interval of 100 micrometers (µm), the histological 5-µm-thick cuts were dyed by hematoxylin-eosin and Masson's trichrome techniques. The glass slides were analyzed under light microscope to examine the neuron morphology. The parameters considered for the morphometric analysis were area, perimeter, maximum diameter, minimum diameter and shape factor of cytoplasm and nucleus of neuron. The results were statistically analyzed by Student’s t test at 5% probability level. The morphological characteristics between the two groups were similar and according to literature. The morphometric results showed that there were changes in neurons size and structure, and increase and loss of star shape were noticed in seropositive animals. The results suggest that the neurons of these dogs, yet nonsymptomatic, can have lost their conductor function. Keywords: canine; central nervous system; histology; Toxoplasma gondii. Este trabalho objetivou analisar a morfologia e a morfometria dos neurônios das regiões cervical, torácica e lombar da medula espinhal de cães assintomáticos soropositivos para toxoplasmose. Utilizaram-se 20 cães sem raça definida, adultos, sendo dez cães hígidos, com sorologia negativa, utilizados como controle (grupo 1) e dez cães assintomáticos mas soropositivos para toxoplasmose (grupo 2). Após microtomia semi-seriada, com intervalos de 100 micrômetros (µm), os cortes histológicos de medula espinhal, à espessura de 5 µm, foram corados pelas técnicas da hematoxilina-eosina e do tricrômico de Masson. As lâminas foram analisadas à microscopia de luz para verificar a morfologia dos neurônios. Para o estudo morfométrico, os parâmetros analisados foram: área, perímetro, diâmetro máximo, diâmetro mínimo e fator de forma do citoplasma e núcleo dos neurônios. Os resultados obtidos foram analisados estatisticamente, mediante o teste t de Student ao nível de 5% de probabilidade. As características morfológicas entre os dois grupos foram semelhantes e em conformidade com a literatura. Os resultados morfométricos demonstraram que há alteração no tamanho e estrutura dos neurônios, com aumento e perda do formato estrelado nos animais soropositivos. Os resultados sugerem que os neurônios destes cães, ainda que assintomáticos, possam ter perdido sua função condutora. Palavras-chave: canino; histologia; sistema nervoso central; Toxoplasma gondii.Universidade Federal de Goiás2015-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1899110.1590/cab.v16i2.18991Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 225-234Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 225-2341809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/18991/18491https://revistas.ufg.br/vet/article/view/18991/18492Carvalho, Alessandra Cristina FrancischiniPacheco, Maria RitaBaraldi-Artoni, Silvana MartinezGirardi, Annita Moraisinfo:eu-repo/semantics/openAccess2015-05-22T12:39:36Zoai:ojs.revistas.ufg.br:article/18991Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:54.329717Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
TOXOPLASMOSE: AVALIAÇÃO MORFOLÓGICA E MORFOMÉTRICA DOS NEURÔNIOS DA MEDULA ESPINHAL DE CÃES SOROPOSITIVOS ASSINTOMÁTICOS
title TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
spellingShingle TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
Carvalho, Alessandra Cristina Francischini
Canine
Central nervous system
Histology
Toxoplasma gondii
Saúde Animal
title_short TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
title_full TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
title_fullStr TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
title_full_unstemmed TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
title_sort TOXOPLASMOSIS: MORPHOLOGICAL AND MORPHOMETRIC EVALUATION OF SPINAL CORD NEURONS FROM NONSYMPTOMATIC SEROPOSITIVE DOGS
author Carvalho, Alessandra Cristina Francischini
author_facet Carvalho, Alessandra Cristina Francischini
Pacheco, Maria Rita
Baraldi-Artoni, Silvana Martinez
Girardi, Annita Morais
author_role author
author2 Pacheco, Maria Rita
Baraldi-Artoni, Silvana Martinez
Girardi, Annita Morais
author2_role author
author
author
dc.contributor.author.fl_str_mv Carvalho, Alessandra Cristina Francischini
Pacheco, Maria Rita
Baraldi-Artoni, Silvana Martinez
Girardi, Annita Morais
dc.subject.por.fl_str_mv Canine
Central nervous system
Histology
Toxoplasma gondii
Saúde Animal
topic Canine
Central nervous system
Histology
Toxoplasma gondii
Saúde Animal
description The aim of this work was to analyze the neuron morphology and morphometry of cervical, thoracic and lumbar areas of nonsymptomatic seropositive dogs’ spinal cord for toxoplasmosis. Twenty indefinite-breed adult dogs were used; ten dogs were healthy, with negative serology for toxoplasmosis, and were used as the control group (group 1), and ten dogs were nonsymptomatic but seropositive for toxoplasmosis (group 2).  After the microtomy, with interval of 100 micrometers (µm), the histological 5-µm-thick cuts were dyed by hematoxylin-eosin and Masson's trichrome techniques. The glass slides were analyzed under light microscope to examine the neuron morphology. The parameters considered for the morphometric analysis were area, perimeter, maximum diameter, minimum diameter and shape factor of cytoplasm and nucleus of neuron. The results were statistically analyzed by Student’s t test at 5% probability level. The morphological characteristics between the two groups were similar and according to literature. The morphometric results showed that there were changes in neurons size and structure, and increase and loss of star shape were noticed in seropositive animals. The results suggest that the neurons of these dogs, yet nonsymptomatic, can have lost their conductor function. Keywords: canine; central nervous system; histology; Toxoplasma gondii. 
publishDate 2015
dc.date.none.fl_str_mv 2015-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/18991
10.1590/cab.v16i2.18991
url https://revistas.ufg.br/vet/article/view/18991
identifier_str_mv 10.1590/cab.v16i2.18991
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/18991/18491
https://revistas.ufg.br/vet/article/view/18991/18492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 225-234
Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 225-234
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874787702472704

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