In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/71762 |
Resumo: | Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning in cattle and buffaloes, generating economic losses related to the death due to heart failure, miscarriage, abortion, stillbirth, and neonatal mortality. The aim of this study was to examine the embryotoxic potential of the aqueous plant extract on in vitro bovine embryos. In vitro study was performed in five replicates of bovine embryo culture assigned in two groups: control, in vitro embryo culture medium without the aqueous plant extract; treated group, with addition of 2.7mg/mL of aqueous plant extract (10%) to the embryo culture on the sixth day of culture. Cleavage rate was evaluated at day 2 of the cell culture. Viability, hatchability and underdevelopment of blastocysts on the seventh, eighth, and ninth days (D7, D8, and D9, respectively) of culture were assessed under stereoscopic microscope. On day 7, blastocysts were submitted to TUNEL assay to determine apoptotic index. In vitro exposure of bovine embryos to of N. multiglandulosa resulted in reduced embryo development and survival, evaluated by dark cytoplasm indicating poor morphology and poor quality with marked reduction of hatchability. We observed a significant reduction of blastocyst production/number of cleaved embryos (60.6% vs 41.5%); reduction of blastocysts production/total number of matured bovine oocytes (35.1% vs 21.3%); and embryonic hatching rates (38.0% vs 10.0%). However, no effects were observed on the apoptotic rate. In conclusion, aqueous extract of N. multiglandulosa leaves reduces bovine embryo viability in vitro, suggesting possible detrimental effects on embryo development.Key words: apoptosis; blastocyst; toxic plant. |
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In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryosToxicidade in vitro da Niedenzuella (Tetrapterys) multiglandulosa em embriões bovinosNiedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning in cattle and buffaloes, generating economic losses related to the death due to heart failure, miscarriage, abortion, stillbirth, and neonatal mortality. The aim of this study was to examine the embryotoxic potential of the aqueous plant extract on in vitro bovine embryos. In vitro study was performed in five replicates of bovine embryo culture assigned in two groups: control, in vitro embryo culture medium without the aqueous plant extract; treated group, with addition of 2.7mg/mL of aqueous plant extract (10%) to the embryo culture on the sixth day of culture. Cleavage rate was evaluated at day 2 of the cell culture. Viability, hatchability and underdevelopment of blastocysts on the seventh, eighth, and ninth days (D7, D8, and D9, respectively) of culture were assessed under stereoscopic microscope. On day 7, blastocysts were submitted to TUNEL assay to determine apoptotic index. In vitro exposure of bovine embryos to of N. multiglandulosa resulted in reduced embryo development and survival, evaluated by dark cytoplasm indicating poor morphology and poor quality with marked reduction of hatchability. We observed a significant reduction of blastocyst production/number of cleaved embryos (60.6% vs 41.5%); reduction of blastocysts production/total number of matured bovine oocytes (35.1% vs 21.3%); and embryonic hatching rates (38.0% vs 10.0%). However, no effects were observed on the apoptotic rate. In conclusion, aqueous extract of N. multiglandulosa leaves reduces bovine embryo viability in vitro, suggesting possible detrimental effects on embryo development.Key words: apoptosis; blastocyst; toxic plant.Niedenzuella (Tetrapterys) multiglandulosa, uma videira encontrada no Brasil, tem sido correlacionada a surtos de intoxicações em bovinos e búfalos, gerando perdas econômicas relacionadas à morte por insuficiência cardíaca, aborto, natimorto e mortalidade neonatal. O objetivo deste estudo foi examinar o potencial embriotóxico do extrato vegetal aquoso em embriões bovinos in vitro. O estudo in vitro foi realizado em cinco repetições de cultura de embriões bovinos distribuídas em dois grupos: controle, meio de cultura de embriões in vitro sem o extrato aquoso da planta; grupo tratado, com adição de 2,7mg / mL de extrato vegetal aquoso (10%) à cultura do embrião no sexto dia de cultivo. A taxa de clivagem foi avaliada no dia 2 da cultura de células. Viabilidade, eclodibilidade e subdesenvolvimento de blastocistos no sétimo, oitavo e nono dia (D7, D8 e D9, respectivamente) de cultura foram avaliados em microscópio estereoscópico. No dia 7, os blastocistos foram submetidos ao ensaio TUNEL para determinar o índice apoptótico. Observamos redução significativa da produção de blastocisto / número de embriões clivados (60,6% vs 41,5%); redução da produção de blastocistos / número total de oócitos bovinos maturados (35,1% vs 21,3%); e taxas de eclosão embrionária (38,0% vs 10,0%). No entanto, nenhum efeito foi observado na taxa de apoptose. Em conclusão, o extrato aquoso das folhas de N. multiglandulosa reduz a viabilidade do embrião bovino in vitro, sugerindo possíveis efeitos prejudiciais no desenvolvimento embrionário.Palavras-chave: apoptose; blastocisto; planta tóxica.Universidade Federal de Goiás2022-05-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/71762Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/71762/38379https://revistas.ufg.br/vet/article/view/71762/38570https://revistas.ufg.br/vet/article/view/71762/38380https://revistas.ufg.br/vet/article/view/71762/38571Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBull, Valquiria Leite, Ana Carolina Botelho, Ana Flávia Machadoda Silva, Rayanne Henrique Santana Silva , Eliane Beatriz MagalhãesSoto-Blanco, BenitoNepomuceno, Anelise de Carvalho Borges, Álan Maia Melo, Marília Martins 2023-03-23T18:43:54Zoai:ojs.revistas.ufg.br:article/71762Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:29.924803Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos Toxicidade in vitro da Niedenzuella (Tetrapterys) multiglandulosa em embriões bovinos |
title |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
spellingShingle |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos Bull, Valquiria |
title_short |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
title_full |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
title_fullStr |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
title_full_unstemmed |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
title_sort |
In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos |
author |
Bull, Valquiria |
author_facet |
Bull, Valquiria Leite, Ana Carolina Botelho, Ana Flávia Machado da Silva, Rayanne Henrique Santana Silva , Eliane Beatriz Magalhães Soto-Blanco, Benito Nepomuceno, Anelise de Carvalho Borges, Álan Maia Melo, Marília Martins |
author_role |
author |
author2 |
Leite, Ana Carolina Botelho, Ana Flávia Machado da Silva, Rayanne Henrique Santana Silva , Eliane Beatriz Magalhães Soto-Blanco, Benito Nepomuceno, Anelise de Carvalho Borges, Álan Maia Melo, Marília Martins |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bull, Valquiria Leite, Ana Carolina Botelho, Ana Flávia Machado da Silva, Rayanne Henrique Santana Silva , Eliane Beatriz Magalhães Soto-Blanco, Benito Nepomuceno, Anelise de Carvalho Borges, Álan Maia Melo, Marília Martins |
description |
Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning in cattle and buffaloes, generating economic losses related to the death due to heart failure, miscarriage, abortion, stillbirth, and neonatal mortality. The aim of this study was to examine the embryotoxic potential of the aqueous plant extract on in vitro bovine embryos. In vitro study was performed in five replicates of bovine embryo culture assigned in two groups: control, in vitro embryo culture medium without the aqueous plant extract; treated group, with addition of 2.7mg/mL of aqueous plant extract (10%) to the embryo culture on the sixth day of culture. Cleavage rate was evaluated at day 2 of the cell culture. Viability, hatchability and underdevelopment of blastocysts on the seventh, eighth, and ninth days (D7, D8, and D9, respectively) of culture were assessed under stereoscopic microscope. On day 7, blastocysts were submitted to TUNEL assay to determine apoptotic index. In vitro exposure of bovine embryos to of N. multiglandulosa resulted in reduced embryo development and survival, evaluated by dark cytoplasm indicating poor morphology and poor quality with marked reduction of hatchability. We observed a significant reduction of blastocyst production/number of cleaved embryos (60.6% vs 41.5%); reduction of blastocysts production/total number of matured bovine oocytes (35.1% vs 21.3%); and embryonic hatching rates (38.0% vs 10.0%). However, no effects were observed on the apoptotic rate. In conclusion, aqueous extract of N. multiglandulosa leaves reduces bovine embryo viability in vitro, suggesting possible detrimental effects on embryo development.Key words: apoptosis; blastocyst; toxic plant. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/71762 |
url |
https://revistas.ufg.br/vet/article/view/71762 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/71762/38379 https://revistas.ufg.br/vet/article/view/71762/38570 https://revistas.ufg.br/vet/article/view/71762/38380 https://revistas.ufg.br/vet/article/view/71762/38571 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874790631145472 |