THE RECOVERY RATES OF EQUINE OOCYTES COMPACT Cumulus oophorus BY SCRAPING THE FOLLICLE WALL

• Autor(a) principal: Curcio, Bruna da Rosa
• Data de Publicação: 2006
• Outros Autores: Frey Jr., Friedrich, Boff, André Luis Nunes, Leon, Priscila Marques Moura de, Lins, Luciana Araújo, Albuquerque, Luís Pedro, Rambo, Gissele, Nogueira, Carlos Eduardo Wayne, Deschamps, João Carlos
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/871

The objective of this study was to describe the steps of the method to recover equine oocytes by scraping the follicle wall and to determine recovery rates of compact Cumulus-oocytes by scraping. Equine ovaries were obtained at a slaughterhouse. After removal of the albuginea tunica, follicles having 10 to 30 mm in diameter were dissected without the ovarian stroma. The follicles were opened individually in Petri dishes, and the granulose cell layer was scraped with a bone currett. Cumulus-oocytes complexes were identified using a dissection microscope and classified according to the Cumulus oophorus morphology and ooplasm appearance. From a total of 527 dissected ovaries, were obtained 3.5 follicles dissected/ ovary and 2.5 oocytes/ovary. The recovery rate was 71.0% (oocytes/follicles), whereas 70.2% of these were classified as having compact Cumulus-oocytes and homogenous ooplasma. The average of compact Cumulus-oocytes per ovary was 1.75. The scraping of the granulose layer of follicle having 10 to 30 mm in diameter allows the recovery of equine oocytes with compact Cumulus, which are considered adequate for in vitro maturation studies. KEY WORDS: Equine oocytes, scraping of follicle wall, in vitro maturation.