EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/4600 |
Resumo: | In order to evaluate the effect of adding Trolox and Glutathione reduced antioxidants on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of French Bulldog (n=1), Basset Hound (n=2) and Rottweiler (n=1) breeds, through digital manipulation. After macroscopic and microscopic evaluation, the volume of semen was divided in aliquots, according to the experiments and experimental groups, using different concentrations of Trolox (200 and 300 mM/L) or GSH (2 and 5 mM/L). The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine and stored in liquid nitrogen. After thawing at 37oC (60 sec) and incubation during 60 minutes, we verified that motility, vigor and sperm with intact acrosomes showed significant difference (P<0.05) only among incubation times, on the three experiments. In Exp. 2, the addition of 2 and 5 mM/L of GSH (G2 and G3) determined higher (P<0.05) percentage of sperm without oxidative stress. Therefore, we could conclude that GSH, on the concentration of 2 and 5 mM/L, can be added to diluent of dog semen cryopreservation.KEYWORDS: Antioxidant; cryopreservation; dog; semen; viability. |
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EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERMEFEITO DA ADIÇÃO DE TROLOX E GLUTATIONA REDUZIDA NA VIABILIDADE in vitro DE ESPERMATOZOIDES DE CÃESAnimal reproductionCriopreservaçãoviabilidadesêmenantioxidantecães.In order to evaluate the effect of adding Trolox and Glutathione reduced antioxidants on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of French Bulldog (n=1), Basset Hound (n=2) and Rottweiler (n=1) breeds, through digital manipulation. After macroscopic and microscopic evaluation, the volume of semen was divided in aliquots, according to the experiments and experimental groups, using different concentrations of Trolox (200 and 300 mM/L) or GSH (2 and 5 mM/L). The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine and stored in liquid nitrogen. After thawing at 37oC (60 sec) and incubation during 60 minutes, we verified that motility, vigor and sperm with intact acrosomes showed significant difference (P<0.05) only among incubation times, on the three experiments. In Exp. 2, the addition of 2 and 5 mM/L of GSH (G2 and G3) determined higher (P<0.05) percentage of sperm without oxidative stress. Therefore, we could conclude that GSH, on the concentration of 2 and 5 mM/L, can be added to diluent of dog semen cryopreservation.KEYWORDS: Antioxidant; cryopreservation; dog; semen; viability.Com o objetivo de avaliar o efeito da adição dos antioxidantes Trolox e Glutationa reduzida (GSH) na viabilidade in vitro de espermatozoides criopreservados de cães, amostras de sêmen foram colhidas de animais das raças Buldogue Francês (n=1), Basset Hound (n=2) e Rottweiler (n=1), por meio de manipulação digital. A seguir, procedeu-se à avaliação macroscópica e microscópica e divisão do volume de sêmen em alíquotas, de acordo com os experimentos e grupos experimentais, utilizando-se diferentes concentrações de Trolox (200 e 300 mM/L) ou GSH (2 e 5 mM/L). As amostras foram envasadas em palhetas (0,25 mL), criopreservadas em máquina de congelação e armazenadas em nitrogênio líquido. Após descongelação a 37 oC (60 seg) e incubação durante 60 minutos, constatou-se que os resultados de motilidade, vigor e espermatozoides com acrossomas íntegros evidenciaram diferença significativa (P<0,05) apenas entre os tempos de incubação, nos três experimentos. No Exp. 2, a adição de 2 e 5 mM/L de GSH (G2 e G3) determinou maior (P<0,05) porcentual de células sem estresse oxidativo. Concluiu-se que GSH, nas concentrações de 2 e 5 mM/L, pode ser adicionada ao diluente de criopreservação do sêmen de cães.PALAVRAS-CHAVE: Antioxidante; cães; criopreservação; sêmen; viabilidade.Universidade Federal de Goiás2013-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionrAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/460010.5216/cab.v14i4.4600Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 4 (2013); 436-447Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 4 (2013); 436-4471809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/4600/15628https://revistas.ufg.br/vet/article/view/4600/15629Peixoto, Paulo C. V. de AlbuquerqueColeto, Zoraide FernandesMoura, Cristiane ScavuzziAlmeida, Felipe CostaSoares, Pierre CastroSilva, Sildivane ValcáciaGuerra, Maria M. Pessoainfo:eu-repo/semantics/openAccess2013-12-17T13:26:53Zoai:ojs.revistas.ufg.br:article/4600Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:16.484216Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM EFEITO DA ADIÇÃO DE TROLOX E GLUTATIONA REDUZIDA NA VIABILIDADE in vitro DE ESPERMATOZOIDES DE CÃES |
title |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
spellingShingle |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM Peixoto, Paulo C. V. de Albuquerque Animal reproduction Criopreservação viabilidade sêmen antioxidante cães. |
title_short |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
title_full |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
title_fullStr |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
title_full_unstemmed |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
title_sort |
EFFECT OF TROLOX AND GLUTATHIONE REDUCED (GSH) ADDITION ON THE in vitro VIABILITY OF DOG CRYOPRESERVED SPERM |
author |
Peixoto, Paulo C. V. de Albuquerque |
author_facet |
Peixoto, Paulo C. V. de Albuquerque Coleto, Zoraide Fernandes Moura, Cristiane Scavuzzi Almeida, Felipe Costa Soares, Pierre Castro Silva, Sildivane Valcácia Guerra, Maria M. Pessoa |
author_role |
author |
author2 |
Coleto, Zoraide Fernandes Moura, Cristiane Scavuzzi Almeida, Felipe Costa Soares, Pierre Castro Silva, Sildivane Valcácia Guerra, Maria M. Pessoa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Peixoto, Paulo C. V. de Albuquerque Coleto, Zoraide Fernandes Moura, Cristiane Scavuzzi Almeida, Felipe Costa Soares, Pierre Castro Silva, Sildivane Valcácia Guerra, Maria M. Pessoa |
dc.subject.por.fl_str_mv |
Animal reproduction Criopreservação viabilidade sêmen antioxidante cães. |
topic |
Animal reproduction Criopreservação viabilidade sêmen antioxidante cães. |
description |
In order to evaluate the effect of adding Trolox and Glutathione reduced antioxidants on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of French Bulldog (n=1), Basset Hound (n=2) and Rottweiler (n=1) breeds, through digital manipulation. After macroscopic and microscopic evaluation, the volume of semen was divided in aliquots, according to the experiments and experimental groups, using different concentrations of Trolox (200 and 300 mM/L) or GSH (2 and 5 mM/L). The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine and stored in liquid nitrogen. After thawing at 37oC (60 sec) and incubation during 60 minutes, we verified that motility, vigor and sperm with intact acrosomes showed significant difference (P<0.05) only among incubation times, on the three experiments. In Exp. 2, the addition of 2 and 5 mM/L of GSH (G2 and G3) determined higher (P<0.05) percentage of sperm without oxidative stress. Therefore, we could conclude that GSH, on the concentration of 2 and 5 mM/L, can be added to diluent of dog semen cryopreservation.KEYWORDS: Antioxidant; cryopreservation; dog; semen; viability. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion r Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/4600 10.5216/cab.v14i4.4600 |
url |
https://revistas.ufg.br/vet/article/view/4600 |
identifier_str_mv |
10.5216/cab.v14i4.4600 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/4600/15628 https://revistas.ufg.br/vet/article/view/4600/15629 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 4 (2013); 436-447 Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 4 (2013); 436-447 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874785068449792 |