EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS

Detalhes bibliográficos
Autor(a) principal: Farrás, Marcel Cavalcanti
Data de Publicação: 2008
Outros Autores: Avanzi, Bruno Ribeiro, Melo, Cely Marini, Dell'Aqua, José Antônio, Papa, Frederico Ozanam
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/521
Resumo: The use of equine cooled transported semen is increasing significantly in the whole world; this is in consequence of the advantages and improvement of the cooling semen technology. The aim of the present study was to compare three commercial extenders for cooling equine semen (Botusemen®; Botu-Turbo® e INRA 96®) using two Brazilian commercial containers (Botu-Box® e Botutainer®) for cooling and storage. Three ejaculates from four stallions from different breeds were used. The samples were evaluated at 0, 6, 12 and 24 hours using CASA for estimate the total and progressive motility and the plasma membrane integrity assessed using fluorescent probes. According to the results, there was no difference on sperm parameters (P>0.05) when comparing both the cooling/storage containers and the extenders used. Thus, is possible to conclude that the extenders and the containers were efficient on maintaining the motility and viability of semen cooled/stored during 24 hours, being a new proposal for equine cooled semen technology.KEY WORDS: Container, cooled semen, equine, extender.
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spelling EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERSEFEITO DE DIFERENTES DILUENTES NA MANUTENÇÃO DAS CARACTERÍSTICAS DO SÊMEN EQÜINO EM DOIS SISTEMAS DE REFRIGERAÇÃO PASSIVASanidade AnimalThe use of equine cooled transported semen is increasing significantly in the whole world; this is in consequence of the advantages and improvement of the cooling semen technology. The aim of the present study was to compare three commercial extenders for cooling equine semen (Botusemen®; Botu-Turbo® e INRA 96®) using two Brazilian commercial containers (Botu-Box® e Botutainer®) for cooling and storage. Three ejaculates from four stallions from different breeds were used. The samples were evaluated at 0, 6, 12 and 24 hours using CASA for estimate the total and progressive motility and the plasma membrane integrity assessed using fluorescent probes. According to the results, there was no difference on sperm parameters (P>0.05) when comparing both the cooling/storage containers and the extenders used. Thus, is possible to conclude that the extenders and the containers were efficient on maintaining the motility and viability of semen cooled/stored during 24 hours, being a new proposal for equine cooled semen technology.KEY WORDS: Container, cooled semen, equine, extender.A utilização da biotecnologia de sêmen eqüino refrigerado e transportado tem apresentado um crescimento significativo na eqüideocultura mundial, no tocante às inúmeras vantagens e inovações que a técnica tem proporcionado. O presente experimento teve como objetivo avaliar diferentes meios de refrigeração (Botu-Semen®; Botu-Turbo® e INRA 96®) em dois sistemas de transporte de sêmen refrigerado (Botu-Box® e Botutainer®). Foram utilizados três ejaculados de quatro garanhões de diferentes raças, submetidos à análise computadorizada da motilidade total e progressiva, bem como da integridade da membrana plasmática, pela técnica de epifluorescência, nos momentos 0, 6, 12 e 24 horas após a colheita. De acordo com os resultados obtidos para os parâmetros espermáticos, não se observaram diferenças significativas entre os sistemas de refrigeração e os meios testados, concluindo-se que tanto os sistemas de refrigeração como os diluentes foram eficazes na manutenção da motilidade e viabilidade espermática, apresentando-se como alternativas à biotecnologia de transporte de sêmen eqüino refrigerado.PALAVRAS-CHAVES: Diluente, eqüino, refrigeração de sêmen, sistema de transporte.Universidade Federal de Goiás2008-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisaapplication/pdfhttps://revistas.ufg.br/vet/article/view/52110.5216/cab.v9i3.521Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 693-699Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 693-6991809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/521/4139Farrás, Marcel CavalcantiAvanzi, Bruno RibeiroMelo, Cely MariniDell'Aqua, José AntônioPapa, Frederico Ozanaminfo:eu-repo/semantics/openAccess2008-10-15T19:36:43Zoai:ojs.revistas.ufg.br:article/521Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:43.411540Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
EFEITO DE DIFERENTES DILUENTES NA MANUTENÇÃO DAS CARACTERÍSTICAS DO SÊMEN EQÜINO EM DOIS SISTEMAS DE REFRIGERAÇÃO PASSIVA
title EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
spellingShingle EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
Farrás, Marcel Cavalcanti
Sanidade Animal
title_short EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
title_full EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
title_fullStr EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
title_full_unstemmed EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
title_sort EFFECT OF DIFFERENT EXTENDERS ON SPERM VIABILITY OF EQUINE SEMEN COOLED IN TWO DIFFERENT CONTAINERS
author Farrás, Marcel Cavalcanti
author_facet Farrás, Marcel Cavalcanti
Avanzi, Bruno Ribeiro
Melo, Cely Marini
Dell'Aqua, José Antônio
Papa, Frederico Ozanam
author_role author
author2 Avanzi, Bruno Ribeiro
Melo, Cely Marini
Dell'Aqua, José Antônio
Papa, Frederico Ozanam
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Farrás, Marcel Cavalcanti
Avanzi, Bruno Ribeiro
Melo, Cely Marini
Dell'Aqua, José Antônio
Papa, Frederico Ozanam
dc.subject.por.fl_str_mv Sanidade Animal
topic Sanidade Animal
description The use of equine cooled transported semen is increasing significantly in the whole world; this is in consequence of the advantages and improvement of the cooling semen technology. The aim of the present study was to compare three commercial extenders for cooling equine semen (Botusemen®; Botu-Turbo® e INRA 96®) using two Brazilian commercial containers (Botu-Box® e Botutainer®) for cooling and storage. Three ejaculates from four stallions from different breeds were used. The samples were evaluated at 0, 6, 12 and 24 hours using CASA for estimate the total and progressive motility and the plasma membrane integrity assessed using fluorescent probes. According to the results, there was no difference on sperm parameters (P>0.05) when comparing both the cooling/storage containers and the extenders used. Thus, is possible to conclude that the extenders and the containers were efficient on maintaining the motility and viability of semen cooled/stored during 24 hours, being a new proposal for equine cooled semen technology.KEY WORDS: Container, cooled semen, equine, extender.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/521
10.5216/cab.v9i3.521
url https://revistas.ufg.br/vet/article/view/521
identifier_str_mv 10.5216/cab.v9i3.521
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/521/4139
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 693-699
Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 693-699
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874782401921024

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