PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY

Detalhes bibliográficos
Autor(a) principal: Duarte, Adriana Mendes
Data de Publicação: 2007
Outros Autores: Diniz, Elmo Gomes, Nascimento, Mara Regina Bueno Mattos, Tavares, Marcelo, Franco, Rossana Vilela Resende
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/1734
Resumo:  The flow-sorting sperm technology is considered as the most promising technique for sexing semen, due to the separation accuracy of around 90%. Thus, the objec-tive of this study was to determinate the effect of Articial Insemination (AI) with semen of Nelore bulls sexed by flow cytometry on the pregnancy rate and its accuracy. Throughout the study, 427 Nelore heifers were inseminated with only one dose of semen as follows: 241 heifers were treated with flow-sexed sperm (3 x 106 total flow-sorting X-chromosome bearing sperm/dose) and 181 were treated with unsexed sperm (10 x 106 total sperm/dose). The heifers were inseminated after detection of natural heat or after syn-chronization treatment. The results obtained for pregnancy rates did not indicate differences between flow-sexed and unsexed sperm and the accuracy in the sex determination presented high levels and reliability (92.4%).KEY-WORDS: Bovine,  Nelore,  spermatozoa,  sexing.
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spelling PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRYTAXA DE PRENHEZ EM NOVILHAS INSEMINADAS COM SÊMEN DE TOUROS DA RAÇA NELORE SEXADOS POR CITOMETRIA DE FLUXOPregnancyInseminationReproduction The flow-sorting sperm technology is considered as the most promising technique for sexing semen, due to the separation accuracy of around 90%. Thus, the objec-tive of this study was to determinate the effect of Articial Insemination (AI) with semen of Nelore bulls sexed by flow cytometry on the pregnancy rate and its accuracy. Throughout the study, 427 Nelore heifers were inseminated with only one dose of semen as follows: 241 heifers were treated with flow-sexed sperm (3 x 106 total flow-sorting X-chromosome bearing sperm/dose) and 181 were treated with unsexed sperm (10 x 106 total sperm/dose). The heifers were inseminated after detection of natural heat or after syn-chronization treatment. The results obtained for pregnancy rates did not indicate differences between flow-sexed and unsexed sperm and the accuracy in the sex determination presented high levels and reliability (92.4%).KEY-WORDS: Bovine,  Nelore,  spermatozoa,  sexing.A citometria de fluxo é considerada a técnica de sexagem de sêmen mais promissora, em virtude de a acui-dade de separação estar em torno de 90%. Dessa forma, o presente estudo objetivou determinar o efeito da IA com sêmen de touros Nelores sexados por citometria de fluxo sobre a taxa de prenhez, bem como sua acurácia. Foram inseminadas 427 novilhas da raça Nelore utilizando uma única dose de sêmen por animal, sendo que em 241 novi-lhas utilizou-se o sêmen sexado por citometria de fluxo com concentrações de 3 x 106 espermatozóides portado-res do cromossomo X/dose e em 186 utilizou-se o sêmen não sexado com concentrações mínimas de 10 x 106 es-permatozóides viáveis/dose. As inseminações ocorreram depois da detecção de cio natural ou após tratamento para sincronização. Os resultados dos índices de prenhez não indicaram diferenças entre o sêmen sexado e o não-sexado e a acurácia na determinação do sexo apresentou níveis elevados de confiabilidade (92,4%).PALAVRAS-CHAVES: Bovino, espermatozóide, Nelore, sexagem.Universidade Federal de Goiás2007-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisaapplication/pdfhttps://revistas.ufg.br/vet/article/view/173410.5216/cab.v8i3.1734Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 8 No. 3 (2007); 567-574Ciência Animal Brasileira / Brazilian Animal Science; v. 8 n. 3 (2007); 567-5741809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1734/1701Duarte, Adriana MendesDiniz, Elmo GomesNascimento, Mara Regina Bueno MattosTavares, MarceloFranco, Rossana Vilela Resendeinfo:eu-repo/semantics/openAccess2007-09-15T15:26:12Zoai:ojs.revistas.ufg.br:article/1734Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:58.767368Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
TAXA DE PRENHEZ EM NOVILHAS INSEMINADAS COM SÊMEN DE TOUROS DA RAÇA NELORE SEXADOS POR CITOMETRIA DE FLUXO
title PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
spellingShingle PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
Duarte, Adriana Mendes
Pregnancy
Insemination
Reproduction
title_short PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
title_full PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
title_fullStr PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
title_full_unstemmed PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
title_sort PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
author Duarte, Adriana Mendes
author_facet Duarte, Adriana Mendes
Diniz, Elmo Gomes
Nascimento, Mara Regina Bueno Mattos
Tavares, Marcelo
Franco, Rossana Vilela Resende
author_role author
author2 Diniz, Elmo Gomes
Nascimento, Mara Regina Bueno Mattos
Tavares, Marcelo
Franco, Rossana Vilela Resende
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Duarte, Adriana Mendes
Diniz, Elmo Gomes
Nascimento, Mara Regina Bueno Mattos
Tavares, Marcelo
Franco, Rossana Vilela Resende
dc.subject.por.fl_str_mv Pregnancy
Insemination
Reproduction
topic Pregnancy
Insemination
Reproduction
description  The flow-sorting sperm technology is considered as the most promising technique for sexing semen, due to the separation accuracy of around 90%. Thus, the objec-tive of this study was to determinate the effect of Articial Insemination (AI) with semen of Nelore bulls sexed by flow cytometry on the pregnancy rate and its accuracy. Throughout the study, 427 Nelore heifers were inseminated with only one dose of semen as follows: 241 heifers were treated with flow-sexed sperm (3 x 106 total flow-sorting X-chromosome bearing sperm/dose) and 181 were treated with unsexed sperm (10 x 106 total sperm/dose). The heifers were inseminated after detection of natural heat or after syn-chronization treatment. The results obtained for pregnancy rates did not indicate differences between flow-sexed and unsexed sperm and the accuracy in the sex determination presented high levels and reliability (92.4%).KEY-WORDS: Bovine,  Nelore,  spermatozoa,  sexing.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/1734
10.5216/cab.v8i3.1734
url https://revistas.ufg.br/vet/article/view/1734
identifier_str_mv 10.5216/cab.v8i3.1734
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/1734/1701
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 8 No. 3 (2007); 567-574
Ciência Animal Brasileira / Brazilian Animal Science; v. 8 n. 3 (2007); 567-574
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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