CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
DOI: | 10.5216/cab.v14i2.12921 |
Texto Completo: | https://revistas.ufg.br/vet/article/view/12921 |
Resumo: | We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm. |
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Ciência animal brasileira (Online) |
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CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMENCORRELAÇÕES ENTRE O TESTE HIPOSMÓTICO E A AVALIAÇÃO CLÁSSICA DO SÊMEN DE CAPRINOSAnimal reproductionCaprinoCorrelaçãoSêmenTeste Hiposmótico.We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm.Avaliou-se a correlação entre a avaliação da integridade funcional de membrana espermática por meio do teste hiposmótico, utilizando-se soluções com diferentes osmolaridades, e as avaliações convencionais do sêmen fresco de caprinos. Foram utilizados 24 ejaculados de três caprinos machos, obtidos por vagina artificial, os quais foram imediatamente submetidos à avaliação clássica. Em seguida, as amostras foram divididas em cinco alíquotas e submetidas ao teste hiposmótico, utilizando-se água destilada (0 mOsm/L) e soluções de citrato de sódio e frutose de diferentes osmolaridades (50; 100; 150 e 200 mOsm/L). A solução de 100 mOsm/L obteve a maior média de espermatozoides reagidos (34,8%) ao teste hiposmótico (P < 0,05); por outro lado, a água destilada foi a que apresentou os menores valores (20,8%). Não foram detectadas correlações significativas entre os espermatozoides reagidos ao teste hiposmótico e as demais características seminais (P > 0,05). Apesar disso, recomenda-se que o teste hiposmótico seja realizado utilizando-se uma solução a base de citrato de sódio e frutose apresentando 100 mOsm/L para a avaliação da integridade funcional da membrana espermática na espécie caprina.PALAVRAS-CHAVE: caprino; espermatozoide; teste hiposmótico.Universidade Federal de Goiás2013-06-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa científicaapplication/pdftext/htmlapplication/pdfhttps://revistas.ufg.br/vet/article/view/1292110.5216/cab.v14i2.12921Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 2 (2013); 216-221Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 2 (2013); 216-2211809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/12921/14538https://revistas.ufg.br/vet/article/view/12921/14539https://revistas.ufg.br/vet/article/view/12921/15447Oliveira, Isabelle R. Sousa deAlves, Heron MedeirosCastelo, Thibério SouzaBezerra, Francisco Silvestre B.Bezerra, Ana Carla D. S.Silva, Alexandre Rodriguesinfo:eu-repo/semantics/openAccess2013-06-28T13:39:57Zoai:ojs.revistas.ufg.br:article/12921Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:46.256850Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN CORRELAÇÕES ENTRE O TESTE HIPOSMÓTICO E A AVALIAÇÃO CLÁSSICA DO SÊMEN DE CAPRINOS |
title |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
spellingShingle |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN Oliveira, Isabelle R. Sousa de Animal reproduction Caprino Correlação Sêmen Teste Hiposmótico. Oliveira, Isabelle R. Sousa de Animal reproduction Caprino Correlação Sêmen Teste Hiposmótico. |
title_short |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
title_full |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
title_fullStr |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
title_full_unstemmed |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
title_sort |
CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN |
author |
Oliveira, Isabelle R. Sousa de |
author_facet |
Oliveira, Isabelle R. Sousa de Oliveira, Isabelle R. Sousa de Alves, Heron Medeiros Castelo, Thibério Souza Bezerra, Francisco Silvestre B. Bezerra, Ana Carla D. S. Silva, Alexandre Rodrigues Alves, Heron Medeiros Castelo, Thibério Souza Bezerra, Francisco Silvestre B. Bezerra, Ana Carla D. S. Silva, Alexandre Rodrigues |
author_role |
author |
author2 |
Alves, Heron Medeiros Castelo, Thibério Souza Bezerra, Francisco Silvestre B. Bezerra, Ana Carla D. S. Silva, Alexandre Rodrigues |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Isabelle R. Sousa de Alves, Heron Medeiros Castelo, Thibério Souza Bezerra, Francisco Silvestre B. Bezerra, Ana Carla D. S. Silva, Alexandre Rodrigues |
dc.subject.por.fl_str_mv |
Animal reproduction Caprino Correlação Sêmen Teste Hiposmótico. |
topic |
Animal reproduction Caprino Correlação Sêmen Teste Hiposmótico. |
description |
We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/12921 10.5216/cab.v14i2.12921 |
url |
https://revistas.ufg.br/vet/article/view/12921 |
identifier_str_mv |
10.5216/cab.v14i2.12921 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/12921/14538 https://revistas.ufg.br/vet/article/view/12921/14539 https://revistas.ufg.br/vet/article/view/12921/15447 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 2 (2013); 216-221 Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 2 (2013); 216-221 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1822181520114188288 |
dc.identifier.doi.none.fl_str_mv |
10.5216/cab.v14i2.12921 |