INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/e-46789 |
Resumo: | Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, "in house" VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days.Keywords: Diagnosis; Influenza; Swine; Viral viability |
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INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATIONInfluência das condições de armazenamento do vírus influenza na detecção por RT-qPCR E isolamento viralInfluenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, "in house" VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days.Keywords: Diagnosis; Influenza; Swine; Viral viabilityA infecção de suínos pelo vírus influenza causa perdas significativas na suinocultura e a doença tem implicações consideráveis para a saúde pública. Dessa forma, a rápida detecção viral em amostras biológicas de suínos é importante para a vigilância da influenza. Para o diagnóstico, as condições de manutenção das amostras biológicas (modo de acondicionamento, temperatura e período de acondicionamento), desde a colheita das amostras de suínos até o envio ao laboratório, podem interferir negativamente na detecção viral. Neste estudo foi analisada a viabilidade de uma amostra do vírus influenza A H1N1/2009 isolada de suínos, mantida em diferentes modos de acondicionamento (meio comercial UTM, meio in house VTM e sem meio de manutenção) e diferentes temperaturas (4, 23 e 37 °C) por um período de até 120 horas. As amostras foram avaliadas por RT-qPCR e isolamento em ovos embrionados. Foram observados efeitos significativos (p<0,05) para o modo e período de acondicionamento e da interação entre esses dois fatores com a carga viral. Dessa forma, as amostras biológicas enviadas para diagnóstico de influenza devem ser armazenadas, preferencialmente, em meio de manutenção viral a 4 °C e o tempo decorrido entre a colheita da amostra e a chegada ao laboratório deve ser de, no máximo, três dias.Palavras-chave: Diagnóstico; Influenza; Suínos; Viabilidade viralUniversidade Federal de Goiás2018-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-4678910.1590/cab19046789Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-91809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-46789/25792https://revistas.ufg.br/vet/article/view/e-46789/25793Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessHaach, VanessaGava, DanielleColdebella, ArleiSchaefer, Rejane2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/46789Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:18.325846Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION Influência das condições de armazenamento do vírus influenza na detecção por RT-qPCR E isolamento viral |
title |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
spellingShingle |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION Haach, Vanessa |
title_short |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
title_full |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
title_fullStr |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
title_full_unstemmed |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
title_sort |
INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION |
author |
Haach, Vanessa |
author_facet |
Haach, Vanessa Gava, Danielle Coldebella, Arlei Schaefer, Rejane |
author_role |
author |
author2 |
Gava, Danielle Coldebella, Arlei Schaefer, Rejane |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Haach, Vanessa Gava, Danielle Coldebella, Arlei Schaefer, Rejane |
description |
Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, "in house" VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days.Keywords: Diagnosis; Influenza; Swine; Viral viability |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-46789 10.1590/cab19046789 |
url |
https://revistas.ufg.br/vet/article/view/e-46789 |
identifier_str_mv |
10.1590/cab19046789 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-46789/25792 https://revistas.ufg.br/vet/article/view/e-46789/25793 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Ciência Animal Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Ciência Animal Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9 Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-9 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874789406408704 |