MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL

Detalhes bibliográficos
Autor(a) principal: Alves, Lorena Borges
Data de Publicação: 2011
Outros Autores: Eurides, Duvaldo, Andrade, Lilian Vieira de, Silva, Luiz Antônio F. da, Oliveira, Benito J. N. Alves de, Souza, Luiz Augusto de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/11445
Resumo: Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing,  they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.KEYWORDS: córnea; cryopreserved; rabbit.
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spelling MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOLAVALIAÇÃO MORFOLÓGICA DE CÓRNEAS DE COELHOS CRIOPRESERVADAS COM ETILENOGLICOLAnimal sanitypreservaçãonitrogênio líquidohistomorfometriaRabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing,  they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.KEYWORDS: córnea; cryopreserved; rabbit.Avaliou-se o etileno glicol com criopreservador de córnea de coelhos. Foram colhidas córneas do bulbo ocular direito e esquerdo de 10 cadáveres de coelhos adultos, machos, da raça Nova Zelândia, com massa corporal variando de 4,0kg a 5,0kg. As peças foram obtidas imediatamente após o abate dos animais realizado em abatedouro. As córneas foram removidas com uma margem de 2,0mm de esclera e banhadas em solução fisiológica 0,9%. As córneas dos bulbos oculares direitos foram fixadas em formol, incluídas em parafina e coradas em hematoxilina-eosina para avaliação em microscopia de luz. As dos globos oculares esquerdos foram imersas em frascos criogênicos contendo solução salina tamponada em fosfato, 0,4% de albumina sérica bovina e 1,5 molar de etilenoglicol. Os frascos foram congelados em nitrogênio líquido à temperatura de -196ºC durante um período de 60 dias. Após descongelamento, as córneas foram submetidas à análise macroscópica e mensuradas a partir de imagens digitalizadas. A preservação de córneas de coelhos com etilenoglicol, em nitrogênio líquido, não ocasiona alterações na transparência, estruturais e morfométricas das camadas que compõem a córnea.PALAVRAS-CHAVE: Coelho; córnea; criopreservação.Universidade Federal de Goiás2011-06-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1144510.5216/cab.v12i2.11445Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 2 (2011); 376-381Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 2 (2011); 376-3811809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/11445/9270https://revistas.ufg.br/vet/article/view/11445/9271Alves, Lorena BorgesEurides, DuvaldoAndrade, Lilian Vieira deSilva, Luiz Antônio F. daOliveira, Benito J. N. Alves deSouza, Luiz Augusto deinfo:eu-repo/semantics/openAccess2011-06-27T14:44:54Zoai:ojs.revistas.ufg.br:article/11445Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:45.392548Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
AVALIAÇÃO MORFOLÓGICA DE CÓRNEAS DE COELHOS CRIOPRESERVADAS COM ETILENOGLICOL
title MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
spellingShingle MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
Alves, Lorena Borges
Animal sanity
preservação
nitrogênio líquido
histomorfometria
title_short MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
title_full MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
title_fullStr MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
title_full_unstemmed MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
title_sort MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL
author Alves, Lorena Borges
author_facet Alves, Lorena Borges
Eurides, Duvaldo
Andrade, Lilian Vieira de
Silva, Luiz Antônio F. da
Oliveira, Benito J. N. Alves de
Souza, Luiz Augusto de
author_role author
author2 Eurides, Duvaldo
Andrade, Lilian Vieira de
Silva, Luiz Antônio F. da
Oliveira, Benito J. N. Alves de
Souza, Luiz Augusto de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Lorena Borges
Eurides, Duvaldo
Andrade, Lilian Vieira de
Silva, Luiz Antônio F. da
Oliveira, Benito J. N. Alves de
Souza, Luiz Augusto de
dc.subject.por.fl_str_mv Animal sanity
preservação
nitrogênio líquido
histomorfometria
topic Animal sanity
preservação
nitrogênio líquido
histomorfometria
description Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing,  they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.KEYWORDS: córnea; cryopreserved; rabbit.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/11445
10.5216/cab.v12i2.11445
url https://revistas.ufg.br/vet/article/view/11445
identifier_str_mv 10.5216/cab.v12i2.11445
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/11445/9270
https://revistas.ufg.br/vet/article/view/11445/9271
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 2 (2011); 376-381
Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 2 (2011); 376-381
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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