MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/18439 |
Resumo: | The objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus. |
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MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZILDETECÇÃO MOLECULAR DE Babesia canis vogeli EM CÃES E EM Rhipicephalus sanguineus NA MESORREGIÃO DO OESTE MARANHENSE, NORDESTE BRASILEIROAnimal ParasithologySanidade AnimalThe objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus.Objetivou-se determinar a ocorrência de Babesia canis vogeli em cães e no carrapato vetor de ambiente urbano e rural da microrregião de Imperatriz, mesorregião do Oeste Maranhense, verificando-se os possíveis fatores de risco associados à infecção por esse agente. Foram coletadas amostras de sangue e espécimes de carrapatos de 300 cães provenientes da área urbana e rural da microrregião de Imperatriz. Foram realizadas extração do DNA do sangue e dos carrapatos. Posteriormente, as amostras foram submetidas a ensaios de reação em cadeia pela polimerase, utilizando-se oligonucleotídeos iniciadores subespécie-específico para B. c. vogeli. A PCR a partir de amostras de sangue revelou que 3,33% (10/300) dos cães estavam positivos para B. c. vogeli, sendo que 1,0% (3/300) era da área urbana e 2,33% (7/300) da área rural. Não foram verificadas associações entre ambiente de coleta, grupo racial, sexo e controle de carrapatos; contudo, a taxa de positividade para B. c. vogeli foi maior entre os cães jovens. Os carrapatos coletados foram identificados como Rhipicephalus sanguineus, sendo seis (2,56%) positivos para B. c. vogeli, com 1,28% (3/235) provenientes de cães da área urbana e 1,28% (3/235) de cães da área rural. Este estudo confirmou a presença de B. c. vogeli em cães e em R. sanguineus na área urbana e rural da microrregião de Imperatriz-MA.PALAVRAS-CHAVE: B. canis vogeli; epidemiologia molecular; PCR; R. sanguineus.Universidade Federal de Goiás2012-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1843910.5216/cab.v13i3.18439Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 13 No. 3 (2012); 388-395Ciência Animal Brasileira / Brazilian Animal Science; v. 13 n. 3 (2012); 388-3951809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/18439/11977https://revistas.ufg.br/vet/article/view/18439/11978Silva, Arannadia BarbosaCosta, Andréa PereiraSá, Joicy Cortez deCosta, Francisco BorgesSantos, Ana Clara G dosGuerra, Rita de Maria S. N.de C.info:eu-repo/semantics/openAccess2012-09-29T02:15:59Zoai:ojs.revistas.ufg.br:article/18439Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:53.180965Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL DETECÇÃO MOLECULAR DE Babesia canis vogeli EM CÃES E EM Rhipicephalus sanguineus NA MESORREGIÃO DO OESTE MARANHENSE, NORDESTE BRASILEIRO |
title |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
spellingShingle |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL Silva, Arannadia Barbosa Animal Parasithology Sanidade Animal |
title_short |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
title_full |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
title_fullStr |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
title_full_unstemmed |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
title_sort |
MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL |
author |
Silva, Arannadia Barbosa |
author_facet |
Silva, Arannadia Barbosa Costa, Andréa Pereira Sá, Joicy Cortez de Costa, Francisco Borges Santos, Ana Clara G dos Guerra, Rita de Maria S. N.de C. |
author_role |
author |
author2 |
Costa, Andréa Pereira Sá, Joicy Cortez de Costa, Francisco Borges Santos, Ana Clara G dos Guerra, Rita de Maria S. N.de C. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Arannadia Barbosa Costa, Andréa Pereira Sá, Joicy Cortez de Costa, Francisco Borges Santos, Ana Clara G dos Guerra, Rita de Maria S. N.de C. |
dc.subject.por.fl_str_mv |
Animal Parasithology Sanidade Animal |
topic |
Animal Parasithology Sanidade Animal |
description |
The objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/18439 10.5216/cab.v13i3.18439 |
url |
https://revistas.ufg.br/vet/article/view/18439 |
identifier_str_mv |
10.5216/cab.v13i3.18439 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/18439/11977 https://revistas.ufg.br/vet/article/view/18439/11978 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 13 No. 3 (2012); 388-395 Ciência Animal Brasileira / Brazilian Animal Science; v. 13 n. 3 (2012); 388-395 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874787664723968 |