MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL

Detalhes bibliográficos
Autor(a) principal: Silva, Arannadia Barbosa
Data de Publicação: 2012
Outros Autores: Costa, Andréa Pereira, Sá, Joicy Cortez de, Costa, Francisco Borges, Santos, Ana Clara G dos, Guerra, Rita de Maria S. N.de C.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/18439
Resumo: The objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for  B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of  B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus.
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spelling MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZILDETECÇÃO MOLECULAR DE Babesia canis vogeli EM CÃES E EM Rhipicephalus sanguineus NA MESORREGIÃO DO OESTE MARANHENSE, NORDESTE BRASILEIROAnimal ParasithologySanidade AnimalThe objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for  B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of  B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus.Objetivou-se determinar a ocorrência de Babesia canis vogeli em cães e no carrapato vetor de ambiente urbano e rural da microrregião de Imperatriz, mesorregião do Oeste Maranhense, verificando-se os possíveis fatores de risco associados à infecção por esse agente. Foram coletadas amostras de sangue e espécimes de carrapatos de 300 cães provenientes da área urbana e rural da microrregião de Imperatriz. Foram realizadas extração do DNA do sangue e dos carrapatos. Posteriormente, as amostras foram submetidas a ensaios de reação em cadeia pela polimerase, utilizando-se oligonucleotídeos iniciadores subespécie-específico para B. c. vogeli. A PCR a partir de amostras de sangue revelou que 3,33% (10/300) dos cães estavam positivos para B. c. vogeli, sendo que 1,0% (3/300) era da área urbana e 2,33% (7/300) da área rural. Não foram verificadas associações entre ambiente de coleta, grupo racial, sexo e controle de carrapatos; contudo, a taxa de positividade para B. c. vogeli foi maior entre os cães jovens. Os carrapatos coletados foram identificados como Rhipicephalus sanguineus, sendo seis (2,56%) positivos para B. c. vogeli, com 1,28% (3/235) provenientes de cães da área urbana e 1,28% (3/235) de cães da área rural. Este estudo confirmou a presença de B. c. vogeli em cães e em R. sanguineus na área urbana e rural da microrregião de Imperatriz-MA.PALAVRAS-CHAVE: B. canis vogeli; epidemiologia molecular; PCR; R. sanguineus.Universidade Federal de Goiás2012-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1843910.5216/cab.v13i3.18439Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 13 No. 3 (2012); 388-395Ciência Animal Brasileira / Brazilian Animal Science; v. 13 n. 3 (2012); 388-3951809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/18439/11977https://revistas.ufg.br/vet/article/view/18439/11978Silva, Arannadia BarbosaCosta, Andréa PereiraSá, Joicy Cortez deCosta, Francisco BorgesSantos, Ana Clara G dosGuerra, Rita de Maria S. N.de C.info:eu-repo/semantics/openAccess2012-09-29T02:15:59Zoai:ojs.revistas.ufg.br:article/18439Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:53.180965Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
DETECÇÃO MOLECULAR DE Babesia canis vogeli EM CÃES E EM Rhipicephalus sanguineus NA MESORREGIÃO DO OESTE MARANHENSE, NORDESTE BRASILEIRO
title MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
spellingShingle MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
Silva, Arannadia Barbosa
Animal Parasithology
Sanidade Animal
title_short MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
title_full MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
title_fullStr MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
title_full_unstemmed MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
title_sort MOLECULAR DETECTION OF Babesia canis vogeli IN DOGS AND IN Rhipicephalus sanguineus FROM THE MIDDLE-WEST REGION OF MARANHÃO, NORTHEAST BRAZIL
author Silva, Arannadia Barbosa
author_facet Silva, Arannadia Barbosa
Costa, Andréa Pereira
Sá, Joicy Cortez de
Costa, Francisco Borges
Santos, Ana Clara G dos
Guerra, Rita de Maria S. N.de C.
author_role author
author2 Costa, Andréa Pereira
Sá, Joicy Cortez de
Costa, Francisco Borges
Santos, Ana Clara G dos
Guerra, Rita de Maria S. N.de C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Arannadia Barbosa
Costa, Andréa Pereira
Sá, Joicy Cortez de
Costa, Francisco Borges
Santos, Ana Clara G dos
Guerra, Rita de Maria S. N.de C.
dc.subject.por.fl_str_mv Animal Parasithology
Sanidade Animal
topic Animal Parasithology
Sanidade Animal
description The objective of this study was to determine the occurrence of Babesia canis vogeli in dogs and in the tick vector from urban and rural environment of the microregion of Imperatriz, middle-west of Maranhão, evaluating risk factors associated with infection by this agent. Samples were collected from blood and tick specimens from 300 dogs from urban and rural areas of the microregion of Imperatriz. DNA was extracted from blood samples and ticks, and tested by polymerase chain reaction assays using subspecies-specific initiating oligonucleotides for B. c. vogeli. PCR from the blood revealed that 3.33% (10/300) of dogs were positive for  B. c. vogeli, and that 1.0% (3/300) came from urban and 2.33% (7/300) from rural area. No association was verified in relation to the environment, race group, sex and tick control; however young animals were more frequently positive to the infection. Ticks collected were identified as Rhipicephalus sanguineus, and six (2.56%) were positive for B. c. vogeli, of which 1.28% (3/235) were collected in the urban area and 1.28% (3/235) from dogs in rural areas. This study confirmed the presence of  B. c. vogeli in dogs and in R. sanguineus in urban and rural microregion of Imperatriz-MA.KEYWORDS: B. canis vogeli; molecular epidemiology ; PCR; R. sanguineus.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/18439
10.5216/cab.v13i3.18439
url https://revistas.ufg.br/vet/article/view/18439
identifier_str_mv 10.5216/cab.v13i3.18439
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/18439/11977
https://revistas.ufg.br/vet/article/view/18439/11978
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 13 No. 3 (2012); 388-395
Ciência Animal Brasileira / Brazilian Animal Science; v. 13 n. 3 (2012); 388-395
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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