EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/26124 |
Resumo: | The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol.KEYWORDS: cryopreservation; dimethylformamide; dogs; glycerol; trehalose. |
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EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMENAVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINOAnimal ReproductioncriopreservaçãogliceroldimetilformamidatrealosecãoThe aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol.KEYWORDS: cryopreservation; dimethylformamide; dogs; glycerol; trehalose.O objetivo do trabalho foi avaliar o efeito da associação entre crioprotetores ainda pouco estudados no Brasil como a dimetilformamida e a trealose em meio diluidor, por meio de protocolos de descongelamento rápido e lento. Foram utilizados três cães machos, adultos, sadios da raça Labrador do Retrievier, que foram submetidos a uma coleta de sêmen semanal durante o período de cinco semanas. Os três meios diluentes utilizados neste estudo foram: (D1)-tris-citrato, acrescido de 3% de dimetilformamida + 3% de glicerol, (D2)-3% de dimetilformamida e de trealose e (D3)-4% de glicerol. No descongelamento, metade das amostras de cada meio diluente foi descongelada pelo método rápido, em banho-maria a 75 ºC por sete segundos, seguido de nova imersão a 37 ºC por 1 minuto. A outra metade foi descongelada pelo método lento, em banho-maria à 37 ºC por 1 minuto. O sêmen foi avaliado quanto à motilidade progressiva, vigor espermáticos e integridade de membrana. Pra isso, as amostras foram submetidas aos testes hipo-osmótico e de integridade da membrana plasmática e do acrossoma (fluorescência). Os resultados indicam que o uso do glicerol como crioprotetor em diluidor TRIS proporciona maior eficácia na criopreservação dos espermatozoides da espécie canina, quando comparados com a dimetilformamida associada ao glicerol ou trealose.PALAVRAS-CHAVE: cão; criopreservação; dimetilformamida; glicerol; trealose. Universidade Federal de Goiás2014-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2612410.5216/cab.v15i1.26124Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 1 (2014); 107-114Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 1 (2014); 107-1141809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/26124/16234https://revistas.ufg.br/vet/article/view/26124/16235Acipreste, Amanda CarlaCosta, Eduardo PaulinoOliveira, Fabrício AlbaniCosta, Sanely LourençoSilva, Talita FernandesBeretta, Daniel Cortesinfo:eu-repo/semantics/openAccess2014-03-28T13:07:01Zoai:ojs.revistas.ufg.br:article/26124Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:01.623805Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
title |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
spellingShingle |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN Acipreste, Amanda Carla Animal Reproduction criopreservação glicerol dimetilformamida trealose cão |
title_short |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
title_full |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
title_fullStr |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
title_full_unstemmed |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
title_sort |
EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN |
author |
Acipreste, Amanda Carla |
author_facet |
Acipreste, Amanda Carla Costa, Eduardo Paulino Oliveira, Fabrício Albani Costa, Sanely Lourenço Silva, Talita Fernandes Beretta, Daniel Cortes |
author_role |
author |
author2 |
Costa, Eduardo Paulino Oliveira, Fabrício Albani Costa, Sanely Lourenço Silva, Talita Fernandes Beretta, Daniel Cortes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Acipreste, Amanda Carla Costa, Eduardo Paulino Oliveira, Fabrício Albani Costa, Sanely Lourenço Silva, Talita Fernandes Beretta, Daniel Cortes |
dc.subject.por.fl_str_mv |
Animal Reproduction criopreservação glicerol dimetilformamida trealose cão |
topic |
Animal Reproduction criopreservação glicerol dimetilformamida trealose cão |
description |
The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol.KEYWORDS: cryopreservation; dimethylformamide; dogs; glycerol; trehalose. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/26124 10.5216/cab.v15i1.26124 |
url |
https://revistas.ufg.br/vet/article/view/26124 |
identifier_str_mv |
10.5216/cab.v15i1.26124 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/26124/16234 https://revistas.ufg.br/vet/article/view/26124/16235 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 1 (2014); 107-114 Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 1 (2014); 107-114 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788227809280 |