Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/68909 |
Resumo: | Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% having clinical signs. Splenomegaly and lymphadenomegaly were the main macroscopic changes. All dogs showed changes in the spleen, unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia and spinal cord hyperplasia and hypertrophy were observed in the lymph nodes. Amastigote forms of Leishmania spp. were found in the spleen and lymph node during histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs (p = 0.23) . Thus, the spleen and lymph node reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease. In addition, the spleen and lymph node have a parasitic load similar to those found by direct diagnostic methods. |
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Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological testsAnálises anatomopatológica e imuno-histoquímica do baço e linfonodo de cães soropositivos para leishmaniose em testes sorológicosCanine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% having clinical signs. Splenomegaly and lymphadenomegaly were the main macroscopic changes. All dogs showed changes in the spleen, unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia and spinal cord hyperplasia and hypertrophy were observed in the lymph nodes. Amastigote forms of Leishmania spp. were found in the spleen and lymph node during histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs (p = 0.23) . Thus, the spleen and lymph node reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease. In addition, the spleen and lymph node have a parasitic load similar to those found by direct diagnostic methods.A leishmaniose canina (CanL) é uma zoonose causada pelo protozoário da espécie Leishmania infantum. O baço e os linfonodos sofrem alterações morfológicas durante o CanL. Esta pesquisa teve como objetivo realizar um estudo anatomopatológico e imunohistoquímico desses órgãos em cães reativos para leishmaniose aos testes de Imunoensaio Cromatográfico “Dual Path Platform” (DPP®) e Ensaio de Imunoabsorção Enzimática (ELISA). Vinte e sete cães foram avaliados para exame anatomopatológico com 92,6% possuindo sínais clínicos. A esplenomegalia e a linfadenomegalia foram as principais alterações macroscópicas. Todos os cães apresentaram alterações no baço, não relacionadas à carga parasitária, sendo a esplenite granulomatosa a alteração mais grave. Hiperplasia cortical e paracortical difusa e hiperplasia e hipertrofia das cordas medulares foram observadas nos linfonodos. Formas amastigotas de Leishmania spp. foram encontrados no baço e linfonodo durante as avaliações histopatológica e imunohistoquímica, com boa concordância entre essas avaliações (k = 0,55, p = 0,00124), mas não foi observada diferença na intensidade parasitária desses orgãos (p = 0,23). Assim, baço e linfonodo reativos para leishmaniose aos testes DPP® e ELISA apresentam alterações histomorfológicas decorrentes da doença. Além disso, o baço e o linfonodo apresentam carga parasitária semelhante aos encontrados pelos métodos de diagnóstico direto.Universidade Federal de Goiás2021-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/68909Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/68909/37083https://revistas.ufg.br/vet/article/view/68909/37216https://revistas.ufg.br/vet/article/view/68909/37084https://revistas.ufg.br/vet/article/view/68909/37217Copyright (c) 2021 Ciência Animal Brasileirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira de Magalhães, Aline Maria Bezerra, Lucilandia Pereira Araújo, DiegoSiqueira Gomes de Lima, Bruna do Padro Assunção, Leandrode Paula Nascente, Eduardo Iglesias Santin, Ana PaulaCaldas Menezes, RodrigoBrianezi Dignani de Moura, Veridiana Maria2023-03-23T18:53:00Zoai:ojs.revistas.ufg.br:article/68909Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:27.918488Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests Análises anatomopatológica e imuno-histoquímica do baço e linfonodo de cães soropositivos para leishmaniose em testes sorológicos |
title |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
spellingShingle |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests Oliveira de Magalhães, Aline |
title_short |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
title_full |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
title_fullStr |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
title_full_unstemmed |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
title_sort |
Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
author |
Oliveira de Magalhães, Aline |
author_facet |
Oliveira de Magalhães, Aline Maria Bezerra, Lucilandia Pereira Araújo, Diego Siqueira Gomes de Lima, Bruna do Padro Assunção, Leandro de Paula Nascente, Eduardo Iglesias Santin, Ana Paula Caldas Menezes, Rodrigo Brianezi Dignani de Moura, Veridiana Maria |
author_role |
author |
author2 |
Maria Bezerra, Lucilandia Pereira Araújo, Diego Siqueira Gomes de Lima, Bruna do Padro Assunção, Leandro de Paula Nascente, Eduardo Iglesias Santin, Ana Paula Caldas Menezes, Rodrigo Brianezi Dignani de Moura, Veridiana Maria |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira de Magalhães, Aline Maria Bezerra, Lucilandia Pereira Araújo, Diego Siqueira Gomes de Lima, Bruna do Padro Assunção, Leandro de Paula Nascente, Eduardo Iglesias Santin, Ana Paula Caldas Menezes, Rodrigo Brianezi Dignani de Moura, Veridiana Maria |
description |
Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% having clinical signs. Splenomegaly and lymphadenomegaly were the main macroscopic changes. All dogs showed changes in the spleen, unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia and spinal cord hyperplasia and hypertrophy were observed in the lymph nodes. Amastigote forms of Leishmania spp. were found in the spleen and lymph node during histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs (p = 0.23) . Thus, the spleen and lymph node reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease. In addition, the spleen and lymph node have a parasitic load similar to those found by direct diagnostic methods. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/68909 |
url |
https://revistas.ufg.br/vet/article/view/68909 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/68909/37083 https://revistas.ufg.br/vet/article/view/68909/37216 https://revistas.ufg.br/vet/article/view/68909/37084 https://revistas.ufg.br/vet/article/view/68909/37217 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Ciência Animal Brasileira http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Ciência Animal Brasileira http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874790532579328 |