EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT

Detalhes bibliográficos
Autor(a) principal: dos Santos, Nathalia Fontes de Souto
Data de Publicação: 2016
Outros Autores: Viscardi, Vanessa, Caruso, Anna Clara Porthun, Torres Filho, Rodolpho de Almeida, Alencar, Nayro Xavier de, Lessa, Daniel Augusto Barroso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/31988
Resumo: Tracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash.
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spelling EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNTEFEITO DA FILTRAÇÃO DO LAVADO TRAQUEOBRÔNQUICO DE EQUINOS SOBRE A CONTAGEM DIFERENCIAL DE CÉLULASEquine SanitySanidade de EquinosTracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash.O lavado traqueobrônquico (LTB) é um método usado para recuperar uma amostra de células das vias respiratórias. A citologia do LTB é descrita como uma importante ferramenta no diagnóstico de doença pulmonar em equinos. O excesso de muco geralmente presente no LTB pode alterar a morfologia, dificultando a identificação dos tipos celulares e, por consequência, prejudicando a contagem diferencial de células e a interpretação diagnóstica. O objetivo deste trabalho foi verificar o efeito da filtração do LTB de equinos sobre a contagem diferencial de células na avaliação citológica do lavado traqueobrônquico. Foram utilizados 30 equinos que foram submetidos a coletas de amostras de LTB via endoscópio. De cada amostra obtida foi separada uma primeira alíquota não filtrada (NF). Uma segunda alíquota (F) foi obtida filtrando-se o LTB, utilizando-se uma compressa de gaze hidrófila. As alíquotas F e NF foram submetidas à citocentrifugação para contagem diferencial de 300 células nucleadas. Verificou–se um aumento significativo no percentual de macrófagos e redução significativa de neutrófilos nas amostras filtradas. As alterações observadas foram condizentes com achados de estudos prévios realizados em lavado broncoalveolar. Conclui-se que a filtração do LTB não é uma metodologia aconselhável, pois acarreta em alterações de contagem significativas nas proporções de importantes marcadores celulares de inflamação, podendo gerar possíveis erros de diagnóstico.Palavras-chave: cavalos; citologia; lavado traqueobrônquico.Universidade Federal de Goiás2016-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaAvaliado por paresapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/3198810.1590/cab17231988Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 274-278Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 274-2781809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/vet/article/view/31988/20926https://revistas.ufg.br/vet/article/view/31988/20927dos Santos, Nathalia Fontes de SoutoViscardi, VanessaCaruso, Anna Clara PorthunTorres Filho, Rodolpho de AlmeidaAlencar, Nayro Xavier deLessa, Daniel Augusto Barrosoinfo:eu-repo/semantics/openAccess2016-05-02T11:43:17Zoai:ojs.revistas.ufg.br:article/31988Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:07.387750Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
EFEITO DA FILTRAÇÃO DO LAVADO TRAQUEOBRÔNQUICO DE EQUINOS SOBRE A CONTAGEM DIFERENCIAL DE CÉLULAS
title EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
spellingShingle EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
dos Santos, Nathalia Fontes de Souto
Equine Sanity
Sanidade de Equinos
title_short EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
title_full EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
title_fullStr EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
title_full_unstemmed EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
title_sort EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
author dos Santos, Nathalia Fontes de Souto
author_facet dos Santos, Nathalia Fontes de Souto
Viscardi, Vanessa
Caruso, Anna Clara Porthun
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
author_role author
author2 Viscardi, Vanessa
Caruso, Anna Clara Porthun
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv dos Santos, Nathalia Fontes de Souto
Viscardi, Vanessa
Caruso, Anna Clara Porthun
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
dc.subject.por.fl_str_mv Equine Sanity
Sanidade de Equinos
topic Equine Sanity
Sanidade de Equinos
description Tracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Científica
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/31988
10.1590/cab17231988
url https://revistas.ufg.br/vet/article/view/31988
identifier_str_mv 10.1590/cab17231988
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/31988/20926
https://revistas.ufg.br/vet/article/view/31988/20927
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 274-278
Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 274-278
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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