EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/31988 |
Resumo: | Tracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash. |
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EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNTEFEITO DA FILTRAÇÃO DO LAVADO TRAQUEOBRÔNQUICO DE EQUINOS SOBRE A CONTAGEM DIFERENCIAL DE CÉLULASEquine SanitySanidade de EquinosTracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash.O lavado traqueobrônquico (LTB) é um método usado para recuperar uma amostra de células das vias respiratórias. A citologia do LTB é descrita como uma importante ferramenta no diagnóstico de doença pulmonar em equinos. O excesso de muco geralmente presente no LTB pode alterar a morfologia, dificultando a identificação dos tipos celulares e, por consequência, prejudicando a contagem diferencial de células e a interpretação diagnóstica. O objetivo deste trabalho foi verificar o efeito da filtração do LTB de equinos sobre a contagem diferencial de células na avaliação citológica do lavado traqueobrônquico. Foram utilizados 30 equinos que foram submetidos a coletas de amostras de LTB via endoscópio. De cada amostra obtida foi separada uma primeira alíquota não filtrada (NF). Uma segunda alíquota (F) foi obtida filtrando-se o LTB, utilizando-se uma compressa de gaze hidrófila. As alíquotas F e NF foram submetidas à citocentrifugação para contagem diferencial de 300 células nucleadas. Verificou–se um aumento significativo no percentual de macrófagos e redução significativa de neutrófilos nas amostras filtradas. As alterações observadas foram condizentes com achados de estudos prévios realizados em lavado broncoalveolar. Conclui-se que a filtração do LTB não é uma metodologia aconselhável, pois acarreta em alterações de contagem significativas nas proporções de importantes marcadores celulares de inflamação, podendo gerar possíveis erros de diagnóstico.Palavras-chave: cavalos; citologia; lavado traqueobrônquico.Universidade Federal de Goiás2016-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaAvaliado por paresapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/3198810.1590/cab17231988Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 274-278Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 274-2781809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/vet/article/view/31988/20926https://revistas.ufg.br/vet/article/view/31988/20927dos Santos, Nathalia Fontes de SoutoViscardi, VanessaCaruso, Anna Clara PorthunTorres Filho, Rodolpho de AlmeidaAlencar, Nayro Xavier deLessa, Daniel Augusto Barrosoinfo:eu-repo/semantics/openAccess2016-05-02T11:43:17Zoai:ojs.revistas.ufg.br:article/31988Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:07.387750Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT EFEITO DA FILTRAÇÃO DO LAVADO TRAQUEOBRÔNQUICO DE EQUINOS SOBRE A CONTAGEM DIFERENCIAL DE CÉLULAS |
title |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
spellingShingle |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT dos Santos, Nathalia Fontes de Souto Equine Sanity Sanidade de Equinos |
title_short |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
title_full |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
title_fullStr |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
title_full_unstemmed |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
title_sort |
EQUINE TRACHEOBRONCHIAL WASH FILTRATION AND ITS EFFECTS ON DIFFERENTIAL CELL COUNT |
author |
dos Santos, Nathalia Fontes de Souto |
author_facet |
dos Santos, Nathalia Fontes de Souto Viscardi, Vanessa Caruso, Anna Clara Porthun Torres Filho, Rodolpho de Almeida Alencar, Nayro Xavier de Lessa, Daniel Augusto Barroso |
author_role |
author |
author2 |
Viscardi, Vanessa Caruso, Anna Clara Porthun Torres Filho, Rodolpho de Almeida Alencar, Nayro Xavier de Lessa, Daniel Augusto Barroso |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
dos Santos, Nathalia Fontes de Souto Viscardi, Vanessa Caruso, Anna Clara Porthun Torres Filho, Rodolpho de Almeida Alencar, Nayro Xavier de Lessa, Daniel Augusto Barroso |
dc.subject.por.fl_str_mv |
Equine Sanity Sanidade de Equinos |
topic |
Equine Sanity Sanidade de Equinos |
description |
Tracheobronchial wash (TBW) is a method to recover cell samples from the airways. The cytology of TBW fluid is an important technique for the diagnosis of pulmonary diseases in horses. Excessive mucus in TBW may cause cell damage and morphological changes that hinder cell type recognition, resulting in a misdiagnosis. The aim of this study was to compare the results of differential cell count in a tracheobronchial wash of filtered and non-filtered samples. Endoscopy and TBW procedures were performed in thirty horses. Each TBW sample was split into two aliquots. Two groups were formed: non-filtrated aliquots (NF) and filtrated aliquots (F). The filtration was performed using a hydrophilic gauze pad. After centrifugation, the differential cell count was performed considering 300 nucleated cells. The filtrated aliquots results presented a significant increase of macrophages count and a significant decrease in neutrophils count comparing to the results of non-filtrated aliquots. These findings were consistent with results of filtered bronchoalveolar wash published studies. Therefore, the filtration of TBW is not an efficient method.Keywords: cytology; horses; tracheobronchial wash. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Científica Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/31988 10.1590/cab17231988 |
url |
https://revistas.ufg.br/vet/article/view/31988 |
identifier_str_mv |
10.1590/cab17231988 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/31988/20926 https://revistas.ufg.br/vet/article/view/31988/20927 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 274-278 Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 274-278 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788712251392 |