DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA

Detalhes bibliográficos
Autor(a) principal: Silva, Alexandre Rodrigues
Data de Publicação: 2009
Outros Autores: Fontenele-Neto, José Domingues, Cardoso, Rita de Cássia Soares, Silva, Lúcia Daniel Machado da, Chirinéa, Viviane Helena, Lopes, Maria Denise
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/3119
Resumo: In spite of the advances in transmission electronic microscopy (TEM), there are few studies presenting a systematic description of the canine spermatozoa and they are only focused on sperm cell heads. The goal of the present study was to describe ultrastructural appearance of canine fresh and frozen-thawed spermatozoa, focusing on damages induced by freezing-thawing in different sperm regions. Ten ejaculates from five proven stud dogs (two ejaculates/dog) were collected, evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed six weeks later.  Samples were evaluated for progressive motility, morphology, and for ultrastructural analysis by TEM. Concerning to TEM, the most striking differences between fresh and frozen-thawed samples were observed over the mid-piece since the fresh spermatozoa showed a well preserved mid-piece. However, the frozen-thawed spermatozoa mid-piece showed signs of damage such as mitochondrial vacuolization. In conclusion, freezing-thawing processes using a Tris-egg yolk extender induce ultrastructural damages in head and mid-piece of canine sperm, affecting the mitochondrial ultrastructure besides.KEY WORDS: Cryopreservation, dog, semen, ultrastructure.
id UFG-7_b8588d3841cc7bc61296fe9db63c0593
oai_identifier_str oai:ojs.revistas.ufg.br:article/3119
network_acronym_str UFG-7
network_name_str Ciência animal brasileira (Online)
repository_id_str
spelling DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOADESCRIÇÃO DE DANOS ULTRAESTRUTURAIS EM ESPERMATOZOIDES CANINOS CONGELADOS-DESCONGELADOSReprodução AnimalCãoSêmenCriopreservaçãoUltra-estruturaIn spite of the advances in transmission electronic microscopy (TEM), there are few studies presenting a systematic description of the canine spermatozoa and they are only focused on sperm cell heads. The goal of the present study was to describe ultrastructural appearance of canine fresh and frozen-thawed spermatozoa, focusing on damages induced by freezing-thawing in different sperm regions. Ten ejaculates from five proven stud dogs (two ejaculates/dog) were collected, evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed six weeks later.  Samples were evaluated for progressive motility, morphology, and for ultrastructural analysis by TEM. Concerning to TEM, the most striking differences between fresh and frozen-thawed samples were observed over the mid-piece since the fresh spermatozoa showed a well preserved mid-piece. However, the frozen-thawed spermatozoa mid-piece showed signs of damage such as mitochondrial vacuolization. In conclusion, freezing-thawing processes using a Tris-egg yolk extender induce ultrastructural damages in head and mid-piece of canine sperm, affecting the mitochondrial ultrastructure besides.KEY WORDS: Cryopreservation, dog, semen, ultrastructure.Apesar dos avanços na microscopia eletrônica de transmissão (MET), existem poucos estudos apresentando uma descrição sistemática do espermatozoide canino, os quais estão focados apenas na cabeça espermática. Objetivou-se, com o presente estudo, descrever a aparência ultraestrutural do espermatozoide canino fresco e congelado-descongelado, enfocando os danos induzidos pela congelação e descongelação em diferentes regiões espermáticas. Dez ejaculados obtidos de cinco cães (dois ejaculados por cão) foram coletados, avaliados e diluídos em Tris-gema-glicerol, congelados e armazenados em nitrogênio líquido, e descongelados seis semanas após. Avaliaram-se as amostras quanto à motilidade progressiva, morfologia espermática e análise ultraestrutural por MET. Em relação à MET, as principais diferenças entre o espermatozoide fresco e descongelado foram observadas na peça intermediária, visto que o espermatozoide fresco apresentava uma peça intermediária bem preservada. Entretanto, a peça intermediária dos espermatozoides descongelados mostrava sinais de danos como a vacuolização das mitocôndrias.  Em conclusão, os processos de congelação e descongelação utilizando o diluente Tris-gema induzem danos ultraestruturais na cabeça e peça intermediária de células espermáticas caninas, afetando, inclusive, a ultraestrutura das mitocôndrias. PALAVRAS-CHAVES: Cão, criopreservação, sêmen, ultraestrutura.Universidade Federal de Goiás2009-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa científicaapplication/pdfhttps://revistas.ufg.br/vet/article/view/311910.5216/cab.v10i2.3119Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 2 (2009); 595-601Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 2 (2009); 595-6011809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/3119/4880Silva, Alexandre RodriguesFontenele-Neto, José DominguesCardoso, Rita de Cássia SoaresSilva, Lúcia Daniel Machado daChirinéa, Viviane HelenaLopes, Maria Deniseinfo:eu-repo/semantics/openAccess2009-07-03T14:37:09Zoai:ojs.revistas.ufg.br:article/3119Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:05.633220Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
DESCRIÇÃO DE DANOS ULTRAESTRUTURAIS EM ESPERMATOZOIDES CANINOS CONGELADOS-DESCONGELADOS
title DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
spellingShingle DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
Silva, Alexandre Rodrigues
Reprodução Animal
Cão
Sêmen
Criopreservação
Ultra-estrutura
title_short DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
title_full DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
title_fullStr DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
title_full_unstemmed DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
title_sort DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA
author Silva, Alexandre Rodrigues
author_facet Silva, Alexandre Rodrigues
Fontenele-Neto, José Domingues
Cardoso, Rita de Cássia Soares
Silva, Lúcia Daniel Machado da
Chirinéa, Viviane Helena
Lopes, Maria Denise
author_role author
author2 Fontenele-Neto, José Domingues
Cardoso, Rita de Cássia Soares
Silva, Lúcia Daniel Machado da
Chirinéa, Viviane Helena
Lopes, Maria Denise
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Alexandre Rodrigues
Fontenele-Neto, José Domingues
Cardoso, Rita de Cássia Soares
Silva, Lúcia Daniel Machado da
Chirinéa, Viviane Helena
Lopes, Maria Denise
dc.subject.por.fl_str_mv Reprodução Animal
Cão
Sêmen
Criopreservação
Ultra-estrutura
topic Reprodução Animal
Cão
Sêmen
Criopreservação
Ultra-estrutura
description In spite of the advances in transmission electronic microscopy (TEM), there are few studies presenting a systematic description of the canine spermatozoa and they are only focused on sperm cell heads. The goal of the present study was to describe ultrastructural appearance of canine fresh and frozen-thawed spermatozoa, focusing on damages induced by freezing-thawing in different sperm regions. Ten ejaculates from five proven stud dogs (two ejaculates/dog) were collected, evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed six weeks later.  Samples were evaluated for progressive motility, morphology, and for ultrastructural analysis by TEM. Concerning to TEM, the most striking differences between fresh and frozen-thawed samples were observed over the mid-piece since the fresh spermatozoa showed a well preserved mid-piece. However, the frozen-thawed spermatozoa mid-piece showed signs of damage such as mitochondrial vacuolization. In conclusion, freezing-thawing processes using a Tris-egg yolk extender induce ultrastructural damages in head and mid-piece of canine sperm, affecting the mitochondrial ultrastructure besides.KEY WORDS: Cryopreservation, dog, semen, ultrastructure.
publishDate 2009
dc.date.none.fl_str_mv 2009-07-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/3119
10.5216/cab.v10i2.3119
url https://revistas.ufg.br/vet/article/view/3119
identifier_str_mv 10.5216/cab.v10i2.3119
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/3119/4880
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 2 (2009); 595-601
Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 2 (2009); 595-601
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874784368001024

Registros relacionados