ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING

Detalhes bibliográficos
Autor(a) principal: Victor Rodrigues Silva, Herlon
Data de Publicação: 2018
Outros Autores: Gothardo Pereira Nunes, Thalles, Cavalcante Mota Filho, Antônio, Nicodemos Pinto, José, Rodrigues Silva, Alexandre, Daniel Machado da Silva, Lúcia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/e-45789
Resumo: This study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week, the samples were thawed and evaluated by computer analysis, as well as for membrane functionality and sperm morphology. Progressive motility in the 2.0 mM BHT group was significantly higher (P <0.05) than that of the 0 mM BHT group (27.6 ± 11.7% vs. 19.0 ± 9.5%, respectively). Regarding all other parameters evaluated, there was no difference between the groups tested. Therefore, the addition of BHT to the ACP-106c extender did not affect the quality of canine semen after thawing.Keywords: antioxidants; cryopreservation; dog; extenders.
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spelling ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZINGAdição de hidroxitolueno butilado (BHT) no diluidor ACP-106c para congelação de sêmen caninoAnimal ReproductionAntioxidantesCãoCriopreservaçãoDiluidoresThis study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week, the samples were thawed and evaluated by computer analysis, as well as for membrane functionality and sperm morphology. Progressive motility in the 2.0 mM BHT group was significantly higher (P <0.05) than that of the 0 mM BHT group (27.6 ± 11.7% vs. 19.0 ± 9.5%, respectively). Regarding all other parameters evaluated, there was no difference between the groups tested. Therefore, the addition of BHT to the ACP-106c extender did not affect the quality of canine semen after thawing.Keywords: antioxidants; cryopreservation; dog; extenders.Este estudo foi realizado para determinar o efeito de hidroxitolueno butilado (BHT) sobre a qualidade do sêmen canino congelado e descongelado, utilizando o diluidor à base de água de coco em pó (ACP-106c). Para tanto, foram realizadas quinze coletas de sêmen provenientes de cinco cães. O sêmen obtido foi diluído em ACP-106c acrescido de glicerol e gema de ovo. As amostras foram então transferidas para tubos contendo diferentes concentrações de BHT (0; 0,5; 1,0 e 2,0 mM). Em seguida, as amostras foram envasadas, congeladas e armazenadas em nitrogênio líquido. O sêmen coletado foi avaliado in natura quanto aos seguintes parâmetros: coloração, volume da fração espermática, motilidade total, vigor, concentração, morfologia e funcionalidade de membrana espermática. Após uma semana, as amostras foram descongeladas e avaliadas por meio de análise computadorizada, como também foram realizadas análises da funcionalidade de membrana e da morfologia espermática. A motilidade progressiva no grupo BHT 2,0 mM foi significativamente superior (P < 0,05) do que a do grupo BHT 0 mM (27,6 ± 11,7% vs. 19,0 ± 9,5%, respectivamente). Em todos os demais parâmetros avaliados, não houve diferença entre os grupos testados. Portanto, conclui-se que a adição do BHT ao diluidor ACP-106c não afetou a qualidade do sêmen canino pós-descongelação.Universidade Federal de Goiás2018-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaResearchapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-4578910.1590/cab19045896Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-91809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-45789/25788https://revistas.ufg.br/vet/article/view/e-45789/25789Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessVictor Rodrigues Silva, HerlonGothardo Pereira Nunes, ThallesCavalcante Mota Filho, AntônioNicodemos Pinto, JoséRodrigues Silva, AlexandreDaniel Machado da Silva, Lúcia2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/45896Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:17.675848Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
Adição de hidroxitolueno butilado (BHT) no diluidor ACP-106c para congelação de sêmen canino
title ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
spellingShingle ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
Victor Rodrigues Silva, Herlon
Animal Reproduction
Antioxidantes
Cão
Criopreservação
Diluidores
title_short ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
title_full ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
title_fullStr ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
title_full_unstemmed ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
title_sort ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING
author Victor Rodrigues Silva, Herlon
author_facet Victor Rodrigues Silva, Herlon
Gothardo Pereira Nunes, Thalles
Cavalcante Mota Filho, Antônio
Nicodemos Pinto, José
Rodrigues Silva, Alexandre
Daniel Machado da Silva, Lúcia
author_role author
author2 Gothardo Pereira Nunes, Thalles
Cavalcante Mota Filho, Antônio
Nicodemos Pinto, José
Rodrigues Silva, Alexandre
Daniel Machado da Silva, Lúcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Victor Rodrigues Silva, Herlon
Gothardo Pereira Nunes, Thalles
Cavalcante Mota Filho, Antônio
Nicodemos Pinto, José
Rodrigues Silva, Alexandre
Daniel Machado da Silva, Lúcia
dc.subject.por.fl_str_mv Animal Reproduction
Antioxidantes
Cão
Criopreservação
Diluidores
topic Animal Reproduction
Antioxidantes
Cão
Criopreservação
Diluidores
description This study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week, the samples were thawed and evaluated by computer analysis, as well as for membrane functionality and sperm morphology. Progressive motility in the 2.0 mM BHT group was significantly higher (P <0.05) than that of the 0 mM BHT group (27.6 ± 11.7% vs. 19.0 ± 9.5%, respectively). Regarding all other parameters evaluated, there was no difference between the groups tested. Therefore, the addition of BHT to the ACP-106c extender did not affect the quality of canine semen after thawing.Keywords: antioxidants; cryopreservation; dog; extenders.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Científica
Research
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/e-45789
10.1590/cab19045896
url https://revistas.ufg.br/vet/article/view/e-45789
identifier_str_mv 10.1590/cab19045896
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/e-45789/25788
https://revistas.ufg.br/vet/article/view/e-45789/25789
dc.rights.driver.fl_str_mv Copyright (c) 2018 Ciência Animal Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Ciência Animal Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9
Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-9
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874789377048576

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