IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS

Detalhes bibliográficos
Autor(a) principal: Chaves, Ricardo de Macêdo
Data de Publicação: 2011
Outros Autores: Bezerra, Filipe Queiros Gondim, Lima, Paulo Fernandes de, Rabelo, Marcelo Cavalcanti, Paula-Lopes, Fabíola, Oliveira, Marcos Antônio L. de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/9187
Resumo: This study aimed to determine the in vitro nuclear maturation and cell death by apoptosis in goat oocytes. The ovaries of goats were collected during the dry (October-March) and rainy (April-September) seasons in slaughterhouses and transported to the Laboratory of Reproductive Biotechniques of UFRPE. Twelve repetitions were performed and cumulus oophorus were collected from follicles of 2-6 mm diameter using the technique of "slicing" and selected based on morphology. The experiment contained two groups: Group-1 (not matured), evaluated immediately after collection, and Group-2 (matured in vitro), assessed after 24 hours of maturation in a CO2 incubator, in which 25 oocytes per drop were placed in alkaline maturation (MBM). After collecting the oocytes from G-1 and G-2, the quality was determined by testing the enzyme activity of caspases and DNA fragmentation (TUNEL), using PhiPhiLux-G1D2 reagent, and in group G-2 the quality was only determined after nuclear maturation. During nuclear maturation stages, germinal vesicle, germinal vesicle breakdown and metaphase I and II were evaluated and no significant differences were found (P> 0.05). There was also no significant difference (P> 0.05) in enzymes activity of caspase and DNA fragmentation of oocytes matured and not matured in vitro. Based on the data obtained, it was concluded that rainy and dry seasons have no effect on in vitro nuclear maturation and apoptosis of goat oocytes.KEYWORDS: Apoptosis; caspases; cumulus oophorus complexes; follicles; TUNEL test.
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spelling IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODSMATURAÇÃO NUCLEAR IN VITRO E MORTE CELULAR POR APOPTOSE EM OÓCITOS DE CAPRINOS NOS PERÍODOS SECO E CHUVOSOAnimal ReproductionApoptoseComplexos cumulus oophorusFolículosCaspasesteste TUNELThis study aimed to determine the in vitro nuclear maturation and cell death by apoptosis in goat oocytes. The ovaries of goats were collected during the dry (October-March) and rainy (April-September) seasons in slaughterhouses and transported to the Laboratory of Reproductive Biotechniques of UFRPE. Twelve repetitions were performed and cumulus oophorus were collected from follicles of 2-6 mm diameter using the technique of "slicing" and selected based on morphology. The experiment contained two groups: Group-1 (not matured), evaluated immediately after collection, and Group-2 (matured in vitro), assessed after 24 hours of maturation in a CO2 incubator, in which 25 oocytes per drop were placed in alkaline maturation (MBM). After collecting the oocytes from G-1 and G-2, the quality was determined by testing the enzyme activity of caspases and DNA fragmentation (TUNEL), using PhiPhiLux-G1D2 reagent, and in group G-2 the quality was only determined after nuclear maturation. During nuclear maturation stages, germinal vesicle, germinal vesicle breakdown and metaphase I and II were evaluated and no significant differences were found (P> 0.05). There was also no significant difference (P> 0.05) in enzymes activity of caspase and DNA fragmentation of oocytes matured and not matured in vitro. Based on the data obtained, it was concluded that rainy and dry seasons have no effect on in vitro nuclear maturation and apoptosis of goat oocytes.KEYWORDS: Apoptosis; caspases; cumulus oophorus complexes; follicles; TUNEL test.Este estudo teve como objetivo determinar a maturação nuclear in vitro e a morte celular por apoptose em oócitos caprinos. Os ovários de cabras foram coletados nos períodos seco (outubro a março) e chuvoso (abril a setembro), em abatedouros, e transportados ao Laboratório de Biotécnicas da Reprodução da UFRPE. Foram realizadas 12 repetições e os complexos cumulus oophorus foram colhidos de folículos entre 2 a 6 mm de diâmetro pela técnica de “slicing” e selecionados com base na morfologia. O experimento continha dois grupos: Grupo-1 (não maturados), avaliados logo após a colheita, e Grupo-2 (maturados in vitro), avaliados após a maturação de 24 horas em incubadora de CO2, na qual 25 oócitos foram colocados por gota em meio básico de maturação (MBM). Após a coleta dos oócitos do G-1 e G-2, foi determinada a qualidade, por meio dos testes de atividade das enzimas caspases e o de fragmentação de DNA (TUNEL), com reagente PhiPhiLux-G1D2, sendo que no grupo G-2 a qualidade só foi determinada após maturação nuclear. Avaliou-se durante os estádios de maturação nuclear as fases de vesícula germinativa, rompimento da vesícula germinativa, metáfase I e II, não havendo diferença significativa (P > 0,05). Também não foi encontrada diferença significativa (P > 0,05) na atividade das enzimas caspases e na fragmentação do DNA dos oócitos não maturados e maturados in vitro. Com base nos dados obtidos, concluí-se que os períodos seco e chuvoso não exercem influência na maturação nuclear in vitro e na apoptose de oócitos da espécie caprina.PALAVRAS-CHAVE: apoptose; caspases; complexos cumulus oophorus; folículos; teste TUNEL.Universidade Federal de Goiás2011-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/918710.5216/cab.v12i4.9187Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 4 (2011); 593-601Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 4 (2011); 593-6011809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/9187/10016https://revistas.ufg.br/vet/article/view/9187/10017Chaves, Ricardo de MacêdoBezerra, Filipe Queiros GondimLima, Paulo Fernandes deRabelo, Marcelo CavalcantiPaula-Lopes, FabíolaOliveira, Marcos Antônio L. deinfo:eu-repo/semantics/openAccess2012-01-30T12:21:40Zoai:ojs.revistas.ufg.br:article/9187Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:42.118622Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
MATURAÇÃO NUCLEAR IN VITRO E MORTE CELULAR POR APOPTOSE EM OÓCITOS DE CAPRINOS NOS PERÍODOS SECO E CHUVOSO
title IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
spellingShingle IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
Chaves, Ricardo de Macêdo
Animal Reproduction
Apoptose
Complexos cumulus oophorus
Folículos
Caspases
teste TUNEL
title_short IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
title_full IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
title_fullStr IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
title_full_unstemmed IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
title_sort IN VITRO NUCLEAR MATURATION AND CELL DEATH BY APOPTOSIS IN GOATS OOCYTES IN RAINY AND DRY PERIODS
author Chaves, Ricardo de Macêdo
author_facet Chaves, Ricardo de Macêdo
Bezerra, Filipe Queiros Gondim
Lima, Paulo Fernandes de
Rabelo, Marcelo Cavalcanti
Paula-Lopes, Fabíola
Oliveira, Marcos Antônio L. de
author_role author
author2 Bezerra, Filipe Queiros Gondim
Lima, Paulo Fernandes de
Rabelo, Marcelo Cavalcanti
Paula-Lopes, Fabíola
Oliveira, Marcos Antônio L. de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chaves, Ricardo de Macêdo
Bezerra, Filipe Queiros Gondim
Lima, Paulo Fernandes de
Rabelo, Marcelo Cavalcanti
Paula-Lopes, Fabíola
Oliveira, Marcos Antônio L. de
dc.subject.por.fl_str_mv Animal Reproduction
Apoptose
Complexos cumulus oophorus
Folículos
Caspases
teste TUNEL
topic Animal Reproduction
Apoptose
Complexos cumulus oophorus
Folículos
Caspases
teste TUNEL
description This study aimed to determine the in vitro nuclear maturation and cell death by apoptosis in goat oocytes. The ovaries of goats were collected during the dry (October-March) and rainy (April-September) seasons in slaughterhouses and transported to the Laboratory of Reproductive Biotechniques of UFRPE. Twelve repetitions were performed and cumulus oophorus were collected from follicles of 2-6 mm diameter using the technique of "slicing" and selected based on morphology. The experiment contained two groups: Group-1 (not matured), evaluated immediately after collection, and Group-2 (matured in vitro), assessed after 24 hours of maturation in a CO2 incubator, in which 25 oocytes per drop were placed in alkaline maturation (MBM). After collecting the oocytes from G-1 and G-2, the quality was determined by testing the enzyme activity of caspases and DNA fragmentation (TUNEL), using PhiPhiLux-G1D2 reagent, and in group G-2 the quality was only determined after nuclear maturation. During nuclear maturation stages, germinal vesicle, germinal vesicle breakdown and metaphase I and II were evaluated and no significant differences were found (P> 0.05). There was also no significant difference (P> 0.05) in enzymes activity of caspase and DNA fragmentation of oocytes matured and not matured in vitro. Based on the data obtained, it was concluded that rainy and dry seasons have no effect on in vitro nuclear maturation and apoptosis of goat oocytes.KEYWORDS: Apoptosis; caspases; cumulus oophorus complexes; follicles; TUNEL test.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/9187
10.5216/cab.v12i4.9187
url https://revistas.ufg.br/vet/article/view/9187
identifier_str_mv 10.5216/cab.v12i4.9187
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/9187/10016
https://revistas.ufg.br/vet/article/view/9187/10017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 4 (2011); 593-601
Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 4 (2011); 593-601
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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