IDENTIFICATION OF WILD MAMMALS FROM PANTANAL SUL-MATOGROSSENSE CARRIERS OF Leptospira spp.

• Autor(a) principal: Souto Vieira, Anahi
• Data de Publicação: 2013
• Outros Autores: Rosinha, Grácia Maria Soares, Vasconcellos, Silvio Arruda, Moaris, Zenaide Maria de, Viana, Rosielle Campozano, Oliveira, Carina Elisei de, Soares, Cleber Oliveira, Araújo, Flabio Ribeiro de, Mourão, Guilherme Miranda, Bianchi, Rita de Cassia, Olifiers, Natalie, Rademaker, Vitor, Rocha, Fabiana Lopes, Pellegrin, Aiesca Oliveira
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/17147
• topic: Animal sanity; Sanidade Animal

A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by phylogenetic analysis.KEYWORDS: leptospirosis; Pantanal; PCR; serology; wild mammals.