EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)

Detalhes bibliográficos
Autor(a) principal: Piassi, Ligia Maria
Data de Publicação: 2009
Outros Autores: Corcini, Carine Dahl, Curcio, Bruna da Rosa, Panzardi, Andrea, Martins, Naina Oliveira, Amado, Milton Oliveira, Varela Junior, Antonio Sergio, Lucia Jr, Thomaz, Deschamps, João Carlos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/1200
Resumo: The objective of this study was to evaluate the effect of three disaccharides (sucrose, threalose and lactose) used as nonpenetrating cryoprotectants in extender for mice semen. The parameters evaluated were sperm motility (MOT) and cleavage rate (CLV) after in vitro fertilization in the SWISS-ALBINA and BALB/c lines. The treatments were S1=sucrose; S2=threalose; S3=lactose, for SWISS-ALBINA; and B1=lactose; B2=threalose for BALB/c.  MOT was evaluated after: thawing (THA), centrifugation and re-suspension in P-1 medium (CEN) and after 10 minutes of incubation (10M). The MOT for the SWISS-ALBINA line was higher for S2 (P
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spelling EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)EFEITO DE CRIOPROTETORES IMPERMEÁVEIS SOBRE A VIABILIDADE IN VITRO DE ESPERMATOZOIDES CONGELADOS DE CAMUNDONGOS (Mus musculus) DAS LINHAGENS SWISS-ALBINA e BALB/cAnimal sanitySanidade animalThe objective of this study was to evaluate the effect of three disaccharides (sucrose, threalose and lactose) used as nonpenetrating cryoprotectants in extender for mice semen. The parameters evaluated were sperm motility (MOT) and cleavage rate (CLV) after in vitro fertilization in the SWISS-ALBINA and BALB/c lines. The treatments were S1=sucrose; S2=threalose; S3=lactose, for SWISS-ALBINA; and B1=lactose; B2=threalose for BALB/c.  MOT was evaluated after: thawing (THA), centrifugation and re-suspension in P-1 medium (CEN) and after 10 minutes of incubation (10M). The MOT for the SWISS-ALBINA line was higher for S2 (PO trabalho objetivou avaliar os efeitos dos dissacarídeos sacarose, trealose e lactose, como crioprotetores impermeáveis à membrana plasmática em diluentes para criopreservação de sêmen de camundongos. Para avaliação do sêmen utilizaram-se os seguintes parâmetros: motilidade progressiva (MOT) das células espermáticas, e a taxa de clivagem embrionária (TXCL) obtida por meio de fertilização in vitro, nas linhagens SWISS-ALBINA e BALB/c. Os tratamentos avaliados foram: S1=sacarose; S2=trealose; S3=lactose, para SWISS-ALBINA e B1=lactose; B2=trealose para BALB/c. Avaliou-se a MOT durante as seguintes etapas: descongelação (DES), centrifugação e ressuspensão no meio P-1 (CEN) e após dez minutos de incubação (10M). A MOT no S2 para a linhagem SWISS-ALBINA nas três etapas (47% no DES; 66,5% na CEN e 67,2% no 10M) foi superior (PUniversidade Federal de Goiás2009-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/120010.5216/cab.v10i4.1200Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 4 (2009); 1161-1170Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 4 (2009); 1161-11701809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1200/5933https://revistas.ufg.br/vet/article/view/1200/6027Piassi, Ligia MariaCorcini, Carine DahlCurcio, Bruna da RosaPanzardi, AndreaMartins, Naina OliveiraAmado, Milton OliveiraVarela Junior, Antonio SergioLucia Jr, ThomazDeschamps, João Carlosinfo:eu-repo/semantics/openAccess2009-12-30T11:34:05Zoai:ojs.revistas.ufg.br:article/1200Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:51.720287Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
EFEITO DE CRIOPROTETORES IMPERMEÁVEIS SOBRE A VIABILIDADE IN VITRO DE ESPERMATOZOIDES CONGELADOS DE CAMUNDONGOS (Mus musculus) DAS LINHAGENS SWISS-ALBINA e BALB/c
title EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
spellingShingle EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
Piassi, Ligia Maria
Animal sanity
Sanidade animal
title_short EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
title_full EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
title_fullStr EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
title_full_unstemmed EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
title_sort EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
author Piassi, Ligia Maria
author_facet Piassi, Ligia Maria
Corcini, Carine Dahl
Curcio, Bruna da Rosa
Panzardi, Andrea
Martins, Naina Oliveira
Amado, Milton Oliveira
Varela Junior, Antonio Sergio
Lucia Jr, Thomaz
Deschamps, João Carlos
author_role author
author2 Corcini, Carine Dahl
Curcio, Bruna da Rosa
Panzardi, Andrea
Martins, Naina Oliveira
Amado, Milton Oliveira
Varela Junior, Antonio Sergio
Lucia Jr, Thomaz
Deschamps, João Carlos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Piassi, Ligia Maria
Corcini, Carine Dahl
Curcio, Bruna da Rosa
Panzardi, Andrea
Martins, Naina Oliveira
Amado, Milton Oliveira
Varela Junior, Antonio Sergio
Lucia Jr, Thomaz
Deschamps, João Carlos
dc.subject.por.fl_str_mv Animal sanity
Sanidade animal
topic Animal sanity
Sanidade animal
description The objective of this study was to evaluate the effect of three disaccharides (sucrose, threalose and lactose) used as nonpenetrating cryoprotectants in extender for mice semen. The parameters evaluated were sperm motility (MOT) and cleavage rate (CLV) after in vitro fertilization in the SWISS-ALBINA and BALB/c lines. The treatments were S1=sucrose; S2=threalose; S3=lactose, for SWISS-ALBINA; and B1=lactose; B2=threalose for BALB/c.  MOT was evaluated after: thawing (THA), centrifugation and re-suspension in P-1 medium (CEN) and after 10 minutes of incubation (10M). The MOT for the SWISS-ALBINA line was higher for S2 (P
publishDate 2009
dc.date.none.fl_str_mv 2009-12-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/1200
10.5216/cab.v10i4.1200
url https://revistas.ufg.br/vet/article/view/1200
identifier_str_mv 10.5216/cab.v10i4.1200
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/1200/5933
https://revistas.ufg.br/vet/article/view/1200/6027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 4 (2009); 1161-1170
Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 4 (2009); 1161-1170
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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