USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/46960 |
Resumo: | The aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejection |
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Ciência animal brasileira (Online) |
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USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUTUTILIZAÇÃO DA OCITOCINA EM GADO GIROLANDO: AVALIAÇÃO DA VIA VAGINAL COMO ALTERNATIVA À VIA ENDOVENOSAThe aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejectionO objetivo deste trabalho foi testar a viabilidade da utilização da via vaginal em fêmeas bovinas visando à aplicação de ocitocina a partir de formulações específicas. A ocitocina foi utilizada associada a facilitadores de absorção e na forma injetável (para comparação). Foram produzidas sete formulações, todas com concentração de 5 UI/mL de ocitocina. O delineamento experimental foi em quadrado latino 8x8 (oito dias, oito tratamentos e oito novilhas ciclando). As administrações de ocitocina foram pela via vaginal e a ocitocina injetável pela via endovenosa, contendo 10 UI cada. Amostras de sangue (n=7) foram coletadas de cada animal, no tempo 0, anterior à administração dos tratamentos, e de três em três minutos até 18 minutos. A ocitocina foi extraída do plasma utilizando coluna cromatográfica C-18 e dosada utilizando o kit comercial Oxytocin EIA. Os resultados obtidos foram avaliados por análise estatística não paramétrica utilizando o teste Kruskal Wallis para comparação das médias. A concentração de ocitocina diferiu entre o tempo 0 e o tempo 12 minutos (p<0,05) para a formulação ocitocina em glicerina. Concluiu-se que houve absorção através da via vaginal para essa formulação. Mais estudos serão necessários para seu emprego no manejo com as fêmeas bovinas de forma efetiva. Palavras-chave: vacas, produção de leite, ordenha, ejeção do leite Universidade Federal de Goiás2019-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/46960Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 20 (2019): Continuous publication; 1-9Ciência Animal Brasileira / Brazilian Animal Science; v. 20 (2019): Publicação contínua; 1-91809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/46960/32956https://revistas.ufg.br/vet/article/view/46960/32957Copyright (c) 2019 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessPessôa Netto, Cristina Loureiro MüllerSaturnino, Helton MattanaPaes, Paulo Ricardo de OliveiraDuarte, Igor Dimitri GamaKeller, Kelly MouraViana, Fernando Antonio Bretas2023-03-23T18:49:21Zoai:ojs.revistas.ufg.br:article/46960Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:18.425986Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT UTILIZAÇÃO DA OCITOCINA EM GADO GIROLANDO: AVALIAÇÃO DA VIA VAGINAL COMO ALTERNATIVA À VIA ENDOVENOSA |
title |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
spellingShingle |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT Pessôa Netto, Cristina Loureiro Müller |
title_short |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
title_full |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
title_fullStr |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
title_full_unstemmed |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
title_sort |
USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT |
author |
Pessôa Netto, Cristina Loureiro Müller |
author_facet |
Pessôa Netto, Cristina Loureiro Müller Saturnino, Helton Mattana Paes, Paulo Ricardo de Oliveira Duarte, Igor Dimitri Gama Keller, Kelly Moura Viana, Fernando Antonio Bretas |
author_role |
author |
author2 |
Saturnino, Helton Mattana Paes, Paulo Ricardo de Oliveira Duarte, Igor Dimitri Gama Keller, Kelly Moura Viana, Fernando Antonio Bretas |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pessôa Netto, Cristina Loureiro Müller Saturnino, Helton Mattana Paes, Paulo Ricardo de Oliveira Duarte, Igor Dimitri Gama Keller, Kelly Moura Viana, Fernando Antonio Bretas |
description |
The aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejection |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/46960 |
url |
https://revistas.ufg.br/vet/article/view/46960 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/46960/32956 https://revistas.ufg.br/vet/article/view/46960/32957 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Ciência Animal Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Ciência Animal Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 20 (2019): Continuous publication; 1-9 Ciência Animal Brasileira / Brazilian Animal Science; v. 20 (2019): Publicação contínua; 1-9 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874789411651584 |