USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT

Detalhes bibliográficos
Autor(a) principal: Pessôa Netto, Cristina Loureiro Müller
Data de Publicação: 2019
Outros Autores: Saturnino, Helton Mattana, Paes, Paulo Ricardo de Oliveira, Duarte, Igor Dimitri Gama, Keller, Kelly Moura, Viana, Fernando Antonio Bretas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/46960
Resumo: The aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejection
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spelling USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUTUTILIZAÇÃO DA OCITOCINA EM GADO GIROLANDO: AVALIAÇÃO DA VIA VAGINAL COMO ALTERNATIVA À VIA ENDOVENOSAThe aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejectionO objetivo deste trabalho foi testar a viabilidade da utilização da via vaginal em fêmeas bovinas visando à aplicação de ocitocina a partir de formulações específicas. A ocitocina foi utilizada associada a facilitadores de absorção e na forma injetável (para comparação). Foram produzidas sete formulações, todas com concentração de 5 UI/mL de ocitocina. O delineamento experimental foi em quadrado latino 8x8 (oito dias, oito tratamentos e oito novilhas ciclando). As administrações de ocitocina foram pela via vaginal e a ocitocina injetável pela via endovenosa, contendo 10 UI cada. Amostras de sangue (n=7) foram coletadas de cada animal, no tempo 0, anterior à administração dos tratamentos, e de três em três minutos até 18 minutos. A ocitocina foi extraída do plasma utilizando coluna cromatográfica C-18 e dosada utilizando o kit comercial Oxytocin EIA. Os resultados obtidos foram avaliados por análise estatística não paramétrica utilizando o teste Kruskal Wallis para comparação das médias. A concentração de ocitocina diferiu entre o tempo 0 e o tempo 12 minutos (p<0,05) para a formulação ocitocina em glicerina. Concluiu-se que houve absorção através da via vaginal para essa formulação. Mais estudos serão necessários para seu emprego no manejo com as fêmeas bovinas de forma efetiva. Palavras-chave: vacas, produção de leite, ordenha, ejeção do leite Universidade Federal de Goiás2019-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/46960Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 20 (2019): Continuous publication; 1-9Ciência Animal Brasileira / Brazilian Animal Science; v. 20 (2019): Publicação contínua; 1-91809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/46960/32956https://revistas.ufg.br/vet/article/view/46960/32957Copyright (c) 2019 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessPessôa Netto, Cristina Loureiro MüllerSaturnino, Helton MattanaPaes, Paulo Ricardo de OliveiraDuarte, Igor Dimitri GamaKeller, Kelly MouraViana, Fernando Antonio Bretas2023-03-23T18:49:21Zoai:ojs.revistas.ufg.br:article/46960Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:18.425986Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
UTILIZAÇÃO DA OCITOCINA EM GADO GIROLANDO: AVALIAÇÃO DA VIA VAGINAL COMO ALTERNATIVA À VIA ENDOVENOSA
title USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
spellingShingle USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
Pessôa Netto, Cristina Loureiro Müller
title_short USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
title_full USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
title_fullStr USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
title_full_unstemmed USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
title_sort USE OF OXYTOCIN IN GIROLANDO CATTLE: EVALUATION OF THE VAGINAL ROUT AS AN ALTERNATIVE TO INTRAVENOUS ROUT
author Pessôa Netto, Cristina Loureiro Müller
author_facet Pessôa Netto, Cristina Loureiro Müller
Saturnino, Helton Mattana
Paes, Paulo Ricardo de Oliveira
Duarte, Igor Dimitri Gama
Keller, Kelly Moura
Viana, Fernando Antonio Bretas
author_role author
author2 Saturnino, Helton Mattana
Paes, Paulo Ricardo de Oliveira
Duarte, Igor Dimitri Gama
Keller, Kelly Moura
Viana, Fernando Antonio Bretas
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pessôa Netto, Cristina Loureiro Müller
Saturnino, Helton Mattana
Paes, Paulo Ricardo de Oliveira
Duarte, Igor Dimitri Gama
Keller, Kelly Moura
Viana, Fernando Antonio Bretas
description The aim of this study was to test the feasibility of vaginal route in order to apply oxytocin in female bovines using specific formulations. The oxytocin was used in its injectable form (as a comparison standard) and associated with absorption enhancers. Seven formulations containing 5 IU/ml of oxytocin each were used. The experimental design was an 8x8 latin square (eight days, eight treatments and eight cycling heifers). The formulations were delivered in vaginal route and the injectable oxytocin was applied intravenously, all of them having 10 IU of oxytocin. Blood samples (n=7) were collected from each animal, the first at time 0, prior to the administration of the treatment, and the following every three minutes until 18 minutes. Oxytocin was extracted from plasma using the C18 Sep-Pak column and measured with the commercial kit Oxytocin EIA. The responses were evaluated using the nonparametric ANOVA and the Kruskal Wallis test was used to compare the means. Statistical difference was observed between times 0 and 12 (p<0,05) for the oxytocin plus glycerin formulation, showing that absorption did occur. Nonetheless, it does not suppress the need for further research before it can be effectively used in female bovine management. Key-words: cows, milk yield, milking, milk ejection
publishDate 2019
dc.date.none.fl_str_mv 2019-04-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/46960
url https://revistas.ufg.br/vet/article/view/46960
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/46960/32956
https://revistas.ufg.br/vet/article/view/46960/32957
dc.rights.driver.fl_str_mv Copyright (c) 2019 Ciência Animal Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Ciência Animal Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 20 (2019): Continuous publication; 1-9
Ciência Animal Brasileira / Brazilian Animal Science; v. 20 (2019): Publicação contínua; 1-9
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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