Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/5511 |
Resumo: | Melatonin treatment and blastocoel collapse had been suggested to be potent options to enhance embryo development and viability after cryopreservation of bovine embryos. The present study tested these alternatives with the aim to improve cryotolerance of bovine embryos produced in vitro. First, the effects of melatonin (MEL) were evaluated at three concentrations in Maturation Media (IVM) and/or Culture Media (IVC) (0, 10-7, 10-9, 10-11 M). The results showed that MEL10-9 in IVM could improve slightly the cleavage rate. However, when applied during IVC, MEL10-9 resulted in improved blastocysts rates and reduced numbers of apoptotic cells (NAC), a higher expression of antioxidative genes without changing the expression metabolism-related, placentation and anti-apoptotic genes. After, the MEL treatment giving the best result in first experiment (MEL 10-9 M in IVC) was combined with blastocoel collapse (BC) immediatly before vitrification. The survival and embryo quality were investigated. This experiment confirmed that independent of BC, MEL supplementation in IVC enhanced re-expansion and hatching rates of vitrified embryos. However, embryos cultured without MEL required more time during re-culture for all expansion. Embryos produced with MEL had similar NAC irrespective of vitrification and BC. BC did not affect embryo quality, in terms of the expression of genes involved in metabolism, oxidative stress, cell repair, placentation and implantation. Therefore, this research concluded that: (i) at 10-9 M concentration, MEL used during IVC improved embryo quality and development, and it minimized the oxidative stress and apoptosis in cells; (ii) embryos cultured with melatonin, vitrified and re-cultured can be transfered in less time; (iii) the blastocoel collapse benefited hatching when embryos were cultured with MEL in IVC; (iv) embryos cultured in IVC with MEL showed better quality and viability, and independently of BC. This information has a potential value for researchs on embryo cryotolerance. |
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Gambarini, Maria Lúciahttp://lattes.cnpq.br/4440003524956701Oliveira Filho, Benedito Dias dehttp://lattes.cnpq.br/6171669841505540Martins, Carlos Fredericohttp://lattes.cnpq.br/1432343540761591Gambarini, Maria LúciaLeão, Karen MartinsBiancardi, Manoel FranciscoCosta, Marcos Fernando Oliveira ePorto, Regiani Nascimento Gagnohttp://lattes.cnpq.br/8166915751398867Marques, Thaisa Campos2016-04-26T10:50:25Z2016-02-23MARQUES, T. C. Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro. 2016. 113 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/5511Melatonin treatment and blastocoel collapse had been suggested to be potent options to enhance embryo development and viability after cryopreservation of bovine embryos. The present study tested these alternatives with the aim to improve cryotolerance of bovine embryos produced in vitro. First, the effects of melatonin (MEL) were evaluated at three concentrations in Maturation Media (IVM) and/or Culture Media (IVC) (0, 10-7, 10-9, 10-11 M). The results showed that MEL10-9 in IVM could improve slightly the cleavage rate. However, when applied during IVC, MEL10-9 resulted in improved blastocysts rates and reduced numbers of apoptotic cells (NAC), a higher expression of antioxidative genes without changing the expression metabolism-related, placentation and anti-apoptotic genes. After, the MEL treatment giving the best result in first experiment (MEL 10-9 M in IVC) was combined with blastocoel collapse (BC) immediatly before vitrification. The survival and embryo quality were investigated. This experiment confirmed that independent of BC, MEL supplementation in IVC enhanced re-expansion and hatching rates of vitrified embryos. However, embryos cultured without MEL required more time during re-culture for all expansion. Embryos produced with MEL had similar NAC irrespective of vitrification and BC. BC did not affect embryo quality, in terms of the expression of genes involved in metabolism, oxidative stress, cell repair, placentation and implantation. Therefore, this research concluded that: (i) at 10-9 M concentration, MEL used during IVC improved embryo quality and development, and it minimized the oxidative stress and apoptosis in cells; (ii) embryos cultured with melatonin, vitrified and re-cultured can be transfered in less time; (iii) the blastocoel collapse benefited hatching when embryos were cultured with MEL in IVC; (iv) embryos cultured in IVC with MEL showed better quality and viability, and independently of BC. This information has a potential value for researchs on embryo cryotolerance.O tratamento com melatonina e a retirada do fluido da blastocele de blastocistos tem sido sugeridos como potentes opções para melhorar o desenvolvimento e a viabilidade embrionária após a criopreservação de embriões de bovinos. O presente estudo testou essas alternativas com o objetivo de melhorar a criotolerância de embriões bovinos produzidos in vitro. Em primeiro lugar, os efeitos da melatonina (MEL) foram avaliados em três concentrações no meio de maturação (MIV) e/ou meio de cultivo (CIV) (0, 10-7, 10-9, 10-11 M). Os resultados mostraram que MEL10-9 no MIV pode melhorar significativamente a taxa de clivagem. No entanto, quando aplicado durante o CIV, MEL10-9 resultou em melhores taxas de blastocistos, reduzido número de células apoptóticas (NCA), maior expressão de genes antioxidantes sem alterar a expressão de genes anti-apoptóticos e relacionados ao metabolismo e placentação. Depois, o tratamento de MEL com melhor resultado no primeiro experimento (MEL 10-9 M no CIV) foi combinado com a retirada do fluido da blastocele (RFB) imediatamente antes da vitrificação. Foram investigadas a qualidade e sobrevivência de embriões. Este estudo confirmou que independente da RFB, a suplementação MEL no CIV melhorou a re-expansão e eclosão dos embriões vitrificados. No entanto, embriões cultivados sem MEL necessitou de maior período de recultivo para sua total re-expansão. Embriões produzidos com MEL tiveram similar NCA, independentemente da vitrificação e RFB. A RFB não afetou a qualidade do embrião em termos de expressão de genes envolvidos no metabolismo, estresse oxidativo, reparo celular, placentação e implantação. Portanto, esta investigação conclui que: (i) na concentração de 10-9 M, MEL utilizada no CIV melhorou a qualidade e desenvolvimento do embrião e, minimizou o estresse oxidativo e a apoptose celular; (ii) os embriões cultivados com MEL, vitrificados e re-cultivados podem ser transferidos em menor tempo; (iii) a RFB beneficiou a eclosão quando os embriões foram cultivados com MEL no CIV; (iv) embriões produzidos com MEL no CIV apresentaram melhor qualidade e viabilidade, independentemente da RFB. Esta informação tem um valor potencial para pesquisas sobre criotolerância embrionária.Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2016-04-25T17:01:02Z No. of bitstreams: 2 Tese - Thaisa Campos Marques - 2016.pdf: 2492479 bytes, checksum: b5a2ef86d3f378ebc4e6976e2ca80765 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-04-26T10:50:25Z (GMT) No. of bitstreams: 2 Tese - Thaisa Campos Marques - 2016.pdf: 2492479 bytes, checksum: b5a2ef86d3f378ebc4e6976e2ca80765 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-04-26T10:50:25Z (GMT). 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dc.title.por.fl_str_mv |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
dc.title.alternative.eng.fl_str_mv |
Alternatives to improve the development and the criotolerance of in vitro produced bovine embryos |
title |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
spellingShingle |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro Marques, Thaisa Campos Melatonina Retirada do fluido da blastocele Vitrificação Apoptose celular Criotolerância de embriões bovinos Melatonin Blastocoel collapse Vitrification Cell apoptosis Bovine embryo cryotolerance CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
title_full |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
title_fullStr |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
title_full_unstemmed |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
title_sort |
Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro |
author |
Marques, Thaisa Campos |
author_facet |
Marques, Thaisa Campos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Gambarini, Maria Lúcia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4440003524956701 |
dc.contributor.advisor-co1.fl_str_mv |
Oliveira Filho, Benedito Dias de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6171669841505540 |
dc.contributor.advisor-co2.fl_str_mv |
Martins, Carlos Frederico |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/1432343540761591 |
dc.contributor.referee1.fl_str_mv |
Gambarini, Maria Lúcia |
dc.contributor.referee2.fl_str_mv |
Leão, Karen Martins |
dc.contributor.referee3.fl_str_mv |
Biancardi, Manoel Francisco |
dc.contributor.referee4.fl_str_mv |
Costa, Marcos Fernando Oliveira e |
dc.contributor.referee5.fl_str_mv |
Porto, Regiani Nascimento Gagno |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8166915751398867 |
dc.contributor.author.fl_str_mv |
Marques, Thaisa Campos |
contributor_str_mv |
Gambarini, Maria Lúcia Oliveira Filho, Benedito Dias de Martins, Carlos Frederico Gambarini, Maria Lúcia Leão, Karen Martins Biancardi, Manoel Francisco Costa, Marcos Fernando Oliveira e Porto, Regiani Nascimento Gagno |
dc.subject.por.fl_str_mv |
Melatonina Retirada do fluido da blastocele Vitrificação Apoptose celular Criotolerância de embriões bovinos |
topic |
Melatonina Retirada do fluido da blastocele Vitrificação Apoptose celular Criotolerância de embriões bovinos Melatonin Blastocoel collapse Vitrification Cell apoptosis Bovine embryo cryotolerance CIENCIAS AGRARIAS::ZOOTECNIA |
dc.subject.eng.fl_str_mv |
Melatonin Blastocoel collapse Vitrification Cell apoptosis Bovine embryo cryotolerance |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::ZOOTECNIA |
description |
Melatonin treatment and blastocoel collapse had been suggested to be potent options to enhance embryo development and viability after cryopreservation of bovine embryos. The present study tested these alternatives with the aim to improve cryotolerance of bovine embryos produced in vitro. First, the effects of melatonin (MEL) were evaluated at three concentrations in Maturation Media (IVM) and/or Culture Media (IVC) (0, 10-7, 10-9, 10-11 M). The results showed that MEL10-9 in IVM could improve slightly the cleavage rate. However, when applied during IVC, MEL10-9 resulted in improved blastocysts rates and reduced numbers of apoptotic cells (NAC), a higher expression of antioxidative genes without changing the expression metabolism-related, placentation and anti-apoptotic genes. After, the MEL treatment giving the best result in first experiment (MEL 10-9 M in IVC) was combined with blastocoel collapse (BC) immediatly before vitrification. The survival and embryo quality were investigated. This experiment confirmed that independent of BC, MEL supplementation in IVC enhanced re-expansion and hatching rates of vitrified embryos. However, embryos cultured without MEL required more time during re-culture for all expansion. Embryos produced with MEL had similar NAC irrespective of vitrification and BC. BC did not affect embryo quality, in terms of the expression of genes involved in metabolism, oxidative stress, cell repair, placentation and implantation. Therefore, this research concluded that: (i) at 10-9 M concentration, MEL used during IVC improved embryo quality and development, and it minimized the oxidative stress and apoptosis in cells; (ii) embryos cultured with melatonin, vitrified and re-cultured can be transfered in less time; (iii) the blastocoel collapse benefited hatching when embryos were cultured with MEL in IVC; (iv) embryos cultured in IVC with MEL showed better quality and viability, and independently of BC. This information has a potential value for researchs on embryo cryotolerance. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-04-26T10:50:25Z |
dc.date.issued.fl_str_mv |
2016-02-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARQUES, T. C. Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro. 2016. 113 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/5511 |
identifier_str_mv |
MARQUES, T. C. Alternativas para melhorar o desenvolvimento e a criotolerância de embriões bovinos produzidos in vitro. 2016. 113 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2016. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/5511 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-8695879823182088786 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
1346858981270845602 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Zootecnia (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/f85016f6-36fc-4674-a720-ba3bc2fad6af/download http://repositorio.bc.ufg.br/tede/bitstreams/1348935d-5322-4051-bd92-5dec6ee7f771/download http://repositorio.bc.ufg.br/tede/bitstreams/1140066d-3d1b-41fb-a6c0-fe907a970d8c/download http://repositorio.bc.ufg.br/tede/bitstreams/2b295f45-2e45-4b76-8c8f-57cf96bbcfe9/download http://repositorio.bc.ufg.br/tede/bitstreams/6c5f6492-7ad2-4de1-97f8-d9a09e7bdd7b/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f ef48816a10f2d45f2e2fee2f478e2faf 9da0b6dfac957114c6a7714714b86306 b5a2ef86d3f378ebc4e6976e2ca80765 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044346608517120 |