Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9709 |
Resumo: | Classical human astroviruses are classified into the family Astroviridae, genus Mamastrovirus, and are further classified into eight serotypes / genotypes (HAstV 1-8). These viruses are considered important agents of non-bacterial acute gastroenteritis (GEA), and can infect individuals of all age groups, being predominant in children up to five years of age. The objectives of this study were to evaluate the occurrence and estimate the viral load of classical human astrovirus, and to perform molecular characterization of positive samples from faecal samples obtained from children up to six years of age with symptoms of acute gastroenteritis (vomiting and / or diarrhea, with or without abdominal pain, with or without fever). For this real-time reverse transcription polymerase chain reaction (RT-qPCR)with standard recombinant plasmid curve and specific probes and primers specific for RLA2 genomic region were used, followed by genomic sequencing and phylogenetic analysis of the positive samplesFecal samples were obtained from 250 children attending a referral hospital in Goiânia from May 2014 to April 2015. A global positivity index of 3.2% (8/250) was observed for HAstV in the feces, and of these positive children, 50% (4/8) presented symptoms of GEA. The viral load ranged from 2.8x105 CG / mL to 1.6x1011 CG / mL, with an average of 2.39x1010. The molecular characterization of the positive samples was performed, of which four were sequenced, two as HAstV-1, 1-a lineage and two as HAstV-4, 4-c lineage. The highest detection occurred in the month of May (5/8), and no defined circulation pattern was observed in relation to dry and rainy seasons. The data obtained in this study contributes to a better understanding of the molecular epidemiology of these agents in the children of the region. |
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Souza, Menira Borges de Lima Dias ehttp://lattes.cnpq.br/0054562567103606Souza, Menira Borges de Lima Dias eFiaccadori, Fabíola SouzaBaur, Carmenhttp://lattes.cnpq.br/7373102365142608Barbosa, Gabriela Rodrigues2019-06-13T13:36:38Z2019-04-23BARBOSA, G. R. Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás. 2019. 56 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/9709Classical human astroviruses are classified into the family Astroviridae, genus Mamastrovirus, and are further classified into eight serotypes / genotypes (HAstV 1-8). These viruses are considered important agents of non-bacterial acute gastroenteritis (GEA), and can infect individuals of all age groups, being predominant in children up to five years of age. The objectives of this study were to evaluate the occurrence and estimate the viral load of classical human astrovirus, and to perform molecular characterization of positive samples from faecal samples obtained from children up to six years of age with symptoms of acute gastroenteritis (vomiting and / or diarrhea, with or without abdominal pain, with or without fever). For this real-time reverse transcription polymerase chain reaction (RT-qPCR)with standard recombinant plasmid curve and specific probes and primers specific for RLA2 genomic region were used, followed by genomic sequencing and phylogenetic analysis of the positive samplesFecal samples were obtained from 250 children attending a referral hospital in Goiânia from May 2014 to April 2015. A global positivity index of 3.2% (8/250) was observed for HAstV in the feces, and of these positive children, 50% (4/8) presented symptoms of GEA. The viral load ranged from 2.8x105 CG / mL to 1.6x1011 CG / mL, with an average of 2.39x1010. The molecular characterization of the positive samples was performed, of which four were sequenced, two as HAstV-1, 1-a lineage and two as HAstV-4, 4-c lineage. The highest detection occurred in the month of May (5/8), and no defined circulation pattern was observed in relation to dry and rainy seasons. The data obtained in this study contributes to a better understanding of the molecular epidemiology of these agents in the children of the region.Os astrovírus humanos clássicos são classificados na família Astroviridae, gênero Mamastrovirus, sendo ainda classificados em oito genótipos (HAstV 1-8). Estes vírus são considerados importantes agentes da gastroenterite aguda (GEA) não bacteriana, podendo infectar indivíduos de todas as faixas etárias, sendo predominante em crianças de até cinco anos de idade. Os objetivos deste estudo foram avaliar a ocorrência e estimar a carga viral de astrovírus humanos clássicos e realizar a caracterização molecular das amostras positivas à partir de amostras de fezes, obtidas de crianças de até seis anos com sintomas de gastrenterite aguda (vômito e/ou diarreia, com ou sem dores abdominais, com ou sem febre). Para tal foi utilizada reação em cadeia pela polimerase pós-transcrição reversa em tempo real (RT-qPCR), com curva padrão de plasmídeo recombinante e sondas e iniciadores específicos para a região RLA2 do genoma de HAstV, seguida de sequenciamento genômico e análise filogenética das amostras positivas. Foram obtidas amostras fecais de 250 crianças atendidas em um hospital de referência ao atendimento pediátrico em Goiânia, no período de maio de 2014 a abril de 2015. Foi observado um índice de positividade global de 3,2% (8/250) para HAstV nas fezes, sendo que destas crianças positivas, 50% (4/8) apresentavam sintomas de GEA. A carga viral variou de 2,8x 105 CG/mL a 1,6x1011 CG/mL, com média de 2,39x1010. Foi realizada a caracterização molecular das amostras positivas, das quais quatro foram sequenciadas, sendo classificadas duas HAstV-1, linhagem 1-a e duas HAstV-4, linhagem 4-c. A maior detecção ocorreu no mês de maio (5/8), não sendo observado padrão de circulação definido em relação às estações seca e chuvosa. Os dados obtidos neste estudo vêm a contribuir para o melhor entendimento da epidemiologia molecular destes agentes na população infantil da região.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2019-06-12T15:34:28Z No. of bitstreams: 2 Dissertação - Gabriela Rodrigues Barbosa - 2019.pdf: 6058792 bytes, checksum: a78a4c50a5bd5ee6abddb23ec8c73b25 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-06-13T13:36:38Z (GMT) No. of bitstreams: 2 Dissertação - Gabriela Rodrigues Barbosa - 2019.pdf: 6058792 bytes, checksum: a78a4c50a5bd5ee6abddb23ec8c73b25 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-06-13T13:36:38Z (GMT). No. of bitstreams: 2 Dissertação - Gabriela Rodrigues Barbosa - 2019.pdf: 6058792 bytes, checksum: a78a4c50a5bd5ee6abddb23ec8c73b25 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-04-23Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAstrovírus humanos clássicosPCR em tempo realGastroenteriteCriançasAssintomáticosClassical human astrovirusesGastroenteritisAsymptomaticChildrenBIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIAAstrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiásinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-5087190859350688984600600600600-7769011444564556288-28177127789453379332075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGCC-LICENSElicense_urllicense_urltext/plain; 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dc.title.eng.fl_str_mv |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
title |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
spellingShingle |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás Barbosa, Gabriela Rodrigues Astrovírus humanos clássicos PCR em tempo real Gastroenterite Crianças Assintomáticos Classical human astroviruses Gastroenteritis Asymptomatic Children BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
title_short |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
title_full |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
title_fullStr |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
title_full_unstemmed |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
title_sort |
Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás |
author |
Barbosa, Gabriela Rodrigues |
author_facet |
Barbosa, Gabriela Rodrigues |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Souza, Menira Borges de Lima Dias e |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0054562567103606 |
dc.contributor.referee1.fl_str_mv |
Souza, Menira Borges de Lima Dias e |
dc.contributor.referee2.fl_str_mv |
Fiaccadori, Fabíola Souza |
dc.contributor.referee3.fl_str_mv |
Baur, Carmen |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7373102365142608 |
dc.contributor.author.fl_str_mv |
Barbosa, Gabriela Rodrigues |
contributor_str_mv |
Souza, Menira Borges de Lima Dias e Souza, Menira Borges de Lima Dias e Fiaccadori, Fabíola Souza Baur, Carmen |
dc.subject.por.fl_str_mv |
Astrovírus humanos clássicos PCR em tempo real Gastroenterite Crianças Assintomáticos |
topic |
Astrovírus humanos clássicos PCR em tempo real Gastroenterite Crianças Assintomáticos Classical human astroviruses Gastroenteritis Asymptomatic Children BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
dc.subject.eng.fl_str_mv |
Classical human astroviruses Gastroenteritis Asymptomatic Children |
dc.subject.cnpq.fl_str_mv |
BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
description |
Classical human astroviruses are classified into the family Astroviridae, genus Mamastrovirus, and are further classified into eight serotypes / genotypes (HAstV 1-8). These viruses are considered important agents of non-bacterial acute gastroenteritis (GEA), and can infect individuals of all age groups, being predominant in children up to five years of age. The objectives of this study were to evaluate the occurrence and estimate the viral load of classical human astrovirus, and to perform molecular characterization of positive samples from faecal samples obtained from children up to six years of age with symptoms of acute gastroenteritis (vomiting and / or diarrhea, with or without abdominal pain, with or without fever). For this real-time reverse transcription polymerase chain reaction (RT-qPCR)with standard recombinant plasmid curve and specific probes and primers specific for RLA2 genomic region were used, followed by genomic sequencing and phylogenetic analysis of the positive samplesFecal samples were obtained from 250 children attending a referral hospital in Goiânia from May 2014 to April 2015. A global positivity index of 3.2% (8/250) was observed for HAstV in the feces, and of these positive children, 50% (4/8) presented symptoms of GEA. The viral load ranged from 2.8x105 CG / mL to 1.6x1011 CG / mL, with an average of 2.39x1010. The molecular characterization of the positive samples was performed, of which four were sequenced, two as HAstV-1, 1-a lineage and two as HAstV-4, 4-c lineage. The highest detection occurred in the month of May (5/8), and no defined circulation pattern was observed in relation to dry and rainy seasons. The data obtained in this study contributes to a better understanding of the molecular epidemiology of these agents in the children of the region. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-06-13T13:36:38Z |
dc.date.issued.fl_str_mv |
2019-04-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARBOSA, G. R. Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás. 2019. 56 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2019. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9709 |
identifier_str_mv |
BARBOSA, G. R. Astrovírus humanos clássicos em amostras fecais de crianças atendidas em um hospital de Goiânia, Goiás. 2019. 56 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2019. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9709 |
dc.language.iso.fl_str_mv |
por |
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por |
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600 600 600 600 |
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dc.relation.cnpq.fl_str_mv |
-2817712778945337933 |
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2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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