Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFJF |
| Texto Completo: | http://dx.doi.org/10.1590/0074-02760170484 https://repositorio.ufjf.br/jspui/handle/ufjf/9256 |
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2019-02-27T15:19:56Z2019-02-252019-02-27T15:19:56Z2018-02113417http://dx.doi.org/10.1590/0074-02760170484https://repositorio.ufjf.br/jspui/handle/ufjf/9256-BACKGROUND Candida glabrata ranks second in epidemiological surveillance studies, and is considered one of the main human yeast pathogens. Treatment of Candida infections represents a contemporary public health problem due to the limited availability of an antifungal arsenal, toxicity effects and increasing cases of resistance. C. glabrata presents intrinsic fluconazole resistance and is a significant concern in clinical practice and in hospital environments. OBJECTIVE The aim of this study was to characterise the azole resistance mechanism presented by a C. glabrata clinical isolate from a Brazilian university hospital. METHODS Azole susceptibility assays, chemosensitisation, flow cytometry and mass spectrometry were performed. FINDINGS Our study demonstrated extremely high resistance to all azoles tested: fluconazole, voriconazole, posaconazole and itraconazole. This isolate was chemosensitised by FK506, a classical inhibitor of ABC transporters related to azole resistance, and Rhodamine 6G extrusion was observed. A mass spectrometry assay confirmed the ABC protein identification suggesting the probable role of efflux pumps in this resistance phenotype. MAIN CONCLUSIONS This study emphasizes the importance of ABC proteins and their relation to the resistance mechanism in hospital environments and they may be an important target for the development of compounds able to unsettle drug extrusion.eng--BrasilMemórias do Instituto Oswaldo Cruz-Candida glabrataABC transportersResistanceFK506Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRocha, Debora Afonso SilvaSa, Leandro Figueira Reis dePinto, Ana Carolina CartagenesJunqueira, Maria de LourdesSilva, Emiliana Mandarano daBorges, Ronaldo MohanaFerreira-Pereira, Antonioinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILCharacterisation of an ABC transporter of a resistant.pdf.jpgCharacterisation of an ABC transporter of a resistant.pdf.jpgGenerated Thumbnailimage/jpeg1659https://repositorio.ufjf.br/jspui/bitstream/ufjf/9256/4/Characterisation%20of%20an%20ABC%20transporter%20of%20a%20resistant.pdf.jpg8e90a6a0668a274ba3e2b28ea57ce992MD54ORIGINALCharacterisation of an ABC transporter of a resistant.pdfCharacterisation of an ABC transporter of a resistant.pdfapplication/pdf620325https://repositorio.ufjf.br/jspui/bitstream/ufjf/9256/1/Characterisation%20of%20an%20ABC%20transporter%20of%20a%20resistant.pdfe38049adfec64af266c4b89146f4d184MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82197https://repositorio.ufjf.br/jspui/bitstream/ufjf/9256/2/license.txt000e18a5aee6ca21bb5811ddf55fc37bMD52TEXTCharacterisation of an ABC transporter of a resistant.pdf.txtCharacterisation of an ABC transporter of a resistant.pdf.txtExtracted texttext/plain38334https://repositorio.ufjf.br/jspui/bitstream/ufjf/9256/3/Characterisation%20of%20an%20ABC%20transporter%20of%20a%20resistant.pdf.txt85b428bfd3b55c261fa1304d8c3b7a69MD53ufjf/92562019-06-16 12:39:23.835oai:hermes.cpd.ufjf.br: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Repositório InstitucionalPUBhttps://repositorio.ufjf.br/oai/requestopendoar:2019-06-16T15:39:23Repositório Institucional da UFJF - Universidade Federal de Juiz de Fora (UFJF)false |
| dc.title.pt_BR.fl_str_mv |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| title |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| spellingShingle |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate Rocha, Debora Afonso Silva - Candida glabrata ABC transporters Resistance FK506 |
| title_short |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| title_full |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| title_fullStr |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| title_full_unstemmed |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| title_sort |
Characterisation of an ABC transporter of a resistant Candida glabrata clinical isolate |
| author |
Rocha, Debora Afonso Silva |
| author_facet |
Rocha, Debora Afonso Silva Sa, Leandro Figueira Reis de Pinto, Ana Carolina Cartagenes Junqueira, Maria de Lourdes Silva, Emiliana Mandarano da Borges, Ronaldo Mohana Ferreira-Pereira, Antonio |
| author_role |
author |
| author2 |
Sa, Leandro Figueira Reis de Pinto, Ana Carolina Cartagenes Junqueira, Maria de Lourdes Silva, Emiliana Mandarano da Borges, Ronaldo Mohana Ferreira-Pereira, Antonio |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Rocha, Debora Afonso Silva Sa, Leandro Figueira Reis de Pinto, Ana Carolina Cartagenes Junqueira, Maria de Lourdes Silva, Emiliana Mandarano da Borges, Ronaldo Mohana Ferreira-Pereira, Antonio |
| dc.subject.cnpq.fl_str_mv |
- |
| topic |
- Candida glabrata ABC transporters Resistance FK506 |
| dc.subject.por.fl_str_mv |
Candida glabrata ABC transporters Resistance FK506 |
| description |
- |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018-02 |
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2019-02-27T15:19:56Z |
| dc.date.available.fl_str_mv |
2019-02-25 2019-02-27T15:19:56Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://repositorio.ufjf.br/jspui/handle/ufjf/9256 |
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http://dx.doi.org/10.1590/0074-02760170484 |
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http://dx.doi.org/10.1590/0074-02760170484 https://repositorio.ufjf.br/jspui/handle/ufjf/9256 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Memórias do Instituto Oswaldo Cruz |
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info:eu-repo/semantics/openAccess |
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openAccess |
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- |
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Brasil |
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