Bacteria from freshwater ecosystems: structural aspects and programmed cell death

Detalhes bibliográficos
Autor(a) principal: Silva, Thiago Pereira da
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UFJF
Texto Completo: https://repositorio.ufjf.br/jspui/handle/ufjf/6145
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spelling Melo, Rossana Correa Netto dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728088Y3Amado, André Megalihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765943H8Cardoso, Simone Jaquelinehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4712886E7Farjalla, Vinicius Forteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795337J5Chiarini-Garcia, Heliohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786707T9http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4434643D4Silva, Thiago Pereira da2018-01-29T11:01:37Z2018-11-012018-01-29T11:01:37Z2017-06-09https://repositorio.ufjf.br/jspui/handle/ufjf/6145-Bacteria are important components of the food web structure in aquatic ecosystems in which they influence the flow of carbon and energy. Populations of bacteria in these ecosystems comprise a diverse spectrum of individual cells able to respond to many factors such as nutrient supply, temperature and virus infection, which regulate bacterial life and death. Bacterial death is a key cellular event involved in the control and production of bacteria in aquatic ecosystems with functional meaning in the carbon and nutrient cycles. Therefore, the study of bacterial structural features and cellular mechanisms underlying bacterial death is crucial to understand processes affecting the entire population. However, both bacterial structure and cellular events of death in aquatic ecosystems are still poorly understood. In the present work, we used single cell approaches to study the structural organization of bacteria as well as to characterize cellular processes of death in these organisms. First, by using fluorescence and transmission electron microscopy (TEM), we provided a general panorama of how microscopy techniques, especially TEM, are powerful tools to understand bacterial structure and their responses to environmental stresses. We showed that bacteria from aquatic ecosystems have remarkable ultrastrutural diversity with components such as bacterial envelope of individual cells differing in structure within the same population. Second, we sought to identify and characterize mechanisms of bacterial cell death. Because our TEM analyses revealed morphological signs of apoptosis, a type of program cell death (PCD), in aquatic bacteria directly collected from natural ecosystems, we applied different techniques to detect apoptosis in bacteria cultured from natural samples. We used TEM as well as different probes to detect this type of PCD in cultured bacteria exposed to increased temperature and viral infection, which are recognized inducers of bacterial death. TEM showed, in both situations, ultrastructural changes indicative of apoptosis, such as cell retraction and condensation, similar to those reported for eukaryotic cells. Assays for membrane permeability, DNA fragmentation, phosphatidilserine exposition and caspase activation were significantly increased in treated bacteria compared to the control group. Altogether, our data demonstrate, for the first time, that PCD occur in aquatic bacteria, and that this event may be a basic mechanism for regulation of bacterial communities in these ecosystems.engUniversidade Federal de Juiz de Fora (UFJF)Programa de Pós-graduação em EcologiaUFJFBrasilICB – Instituto de Ciências BiológicasCNPQ::CIENCIAS BIOLOGICASBacteria from freshwater ecosystemsUltrastructureProgrammed cell deathApoptosisElectron microscopyFlow cytometryBacteria from freshwater ecosystems: structural aspects and programmed cell deathinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILthiagopereiradasilva.pdf.jpgthiagopereiradasilva.pdf.jpgGenerated Thumbnailimage/jpeg1226https://repositorio.ufjf.br/jspui/bitstream/ufjf/6145/4/thiagopereiradasilva.pdf.jpg476bc7072d7acf85f6a0fb493ecab557MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-82197https://repositorio.ufjf.br/jspui/bitstream/ufjf/6145/1/license.txt000e18a5aee6ca21bb5811ddf55fc37bMD51ORIGINALthiagopereiradasilva.pdfthiagopereiradasilva.pdfapplication/pdf6820084https://repositorio.ufjf.br/jspui/bitstream/ufjf/6145/2/thiagopereiradasilva.pdf8e77c8a7f421e5bf4e5fce1fd00a24f5MD52TEXTthiagopereiradasilva.pdf.txtthiagopereiradasilva.pdf.txtExtracted texttext/plain262296https://repositorio.ufjf.br/jspui/bitstream/ufjf/6145/3/thiagopereiradasilva.pdf.txtb7a0dc5523bef303bdb64a47910c04daMD53ufjf/61452019-06-16 09:10:38.704oai:hermes.cpd.ufjf.br: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Repositório InstitucionalPUBhttps://repositorio.ufjf.br/oai/requestopendoar:2019-06-16T12:10:38Repositório Institucional da UFJF - Universidade Federal de Juiz de Fora (UFJF)false
dc.title.pt_BR.fl_str_mv Bacteria from freshwater ecosystems: structural aspects and programmed cell death
title Bacteria from freshwater ecosystems: structural aspects and programmed cell death
spellingShingle Bacteria from freshwater ecosystems: structural aspects and programmed cell death
Silva, Thiago Pereira da
CNPQ::CIENCIAS BIOLOGICAS
Bacteria from freshwater ecosystems
Ultrastructure
Programmed cell death
Apoptosis
Electron microscopy
Flow cytometry
title_short Bacteria from freshwater ecosystems: structural aspects and programmed cell death
title_full Bacteria from freshwater ecosystems: structural aspects and programmed cell death
title_fullStr Bacteria from freshwater ecosystems: structural aspects and programmed cell death
title_full_unstemmed Bacteria from freshwater ecosystems: structural aspects and programmed cell death
title_sort Bacteria from freshwater ecosystems: structural aspects and programmed cell death
author Silva, Thiago Pereira da
author_facet Silva, Thiago Pereira da
author_role author
dc.contributor.advisor1.fl_str_mv Melo, Rossana Correa Netto de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728088Y3
dc.contributor.referee1.fl_str_mv Amado, André Megali
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765943H8
dc.contributor.referee2.fl_str_mv Cardoso, Simone Jaqueline
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4712886E7
dc.contributor.referee3.fl_str_mv Farjalla, Vinicius Fortes
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795337J5
dc.contributor.referee4.fl_str_mv Chiarini-Garcia, Helio
dc.contributor.referee4Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786707T9
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4434643D4
dc.contributor.author.fl_str_mv Silva, Thiago Pereira da
contributor_str_mv Melo, Rossana Correa Netto de
Amado, André Megali
Cardoso, Simone Jaqueline
Farjalla, Vinicius Fortes
Chiarini-Garcia, Helio
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS
topic CNPQ::CIENCIAS BIOLOGICAS
Bacteria from freshwater ecosystems
Ultrastructure
Programmed cell death
Apoptosis
Electron microscopy
Flow cytometry
dc.subject.por.fl_str_mv Bacteria from freshwater ecosystems
Ultrastructure
Programmed cell death
Apoptosis
Electron microscopy
Flow cytometry
description -
publishDate 2017
dc.date.issued.fl_str_mv 2017-06-09
dc.date.accessioned.fl_str_mv 2018-01-29T11:01:37Z
dc.date.available.fl_str_mv 2018-11-01
2018-01-29T11:01:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufjf.br/jspui/handle/ufjf/6145
url https://repositorio.ufjf.br/jspui/handle/ufjf/6145
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Juiz de Fora (UFJF)
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ecologia
dc.publisher.initials.fl_str_mv UFJF
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICB – Instituto de Ciências Biológicas
publisher.none.fl_str_mv Universidade Federal de Juiz de Fora (UFJF)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFJF
instname:Universidade Federal de Juiz de Fora (UFJF)
instacron:UFJF
instname_str Universidade Federal de Juiz de Fora (UFJF)
instacron_str UFJF
institution UFJF
reponame_str Repositório Institucional da UFJF
collection Repositório Institucional da UFJF
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