Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFJF |
| Texto Completo: | http://dx.doi.org/10.1590/S0074-02762011000700005 https://repositorio.ufjf.br/jspui/handle/ufjf/8459 |
Resumo: | - |
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2019-01-15T14:11:59Z2019-01-112019-01-15T14:11:59Z20111067808813http://dx.doi.org/10.1590/S0074-02762011000700005https://repositorio.ufjf.br/jspui/handle/ufjf/8459-A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.eng--BrasilMemórias do Instituto Oswaldo Cruz-Schistosoma mansoniNTPDasePotato apyrasePeptideImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMendes, Rita Gabriela Pedrosa RibeiroGusmão, Michélia Antônia do NascimentoMaia, Ana Carolina Ribeiro GomesDetoni, Michelle de LimaPorcino, Gabriane NascimentoSoares, Thais VieiraJuliano, Maria AparecidaJuliano, LuizCoelho, Paulo Marcos ZechLenzi, Henrique LeonelPinto, Priscila FariaVasconcelos, Eveline Gomesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdf.jpgImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdf.jpgGenerated Thumbnailimage/jpeg1715https://repositorio.ufjf.br/jspui/bitstream/ufjf/8459/4/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP.pdf.jpg7247f29549f94885f00ac55351bd5c1aMD54ORIGINALImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdfImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdfapplication/pdf625875https://repositorio.ufjf.br/jspui/bitstream/ufjf/8459/1/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP.pdf8834459fcc11fd965038de2af0b26662MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82197https://repositorio.ufjf.br/jspui/bitstream/ufjf/8459/2/license.txt000e18a5aee6ca21bb5811ddf55fc37bMD52TEXTImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdf.txtImmunostimulatory property of a synthetic peptide belonging to the soluble ATP.pdf.txtExtracted texttext/plain33544https://repositorio.ufjf.br/jspui/bitstream/ufjf/8459/3/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP.pdf.txte9f7ab01ab00499ee790f2a7227dfc0bMD53ufjf/84592019-06-16 08:39:53.431oai:hermes.cpd.ufjf.br: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Repositório InstitucionalPUBhttps://repositorio.ufjf.br/oai/requestopendoar:2019-06-16T11:39:53Repositório Institucional da UFJF - Universidade Federal de Juiz de Fora (UFJF)false |
| dc.title.pt_BR.fl_str_mv |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| spellingShingle |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg Mendes, Rita Gabriela Pedrosa Ribeiro - Schistosoma mansoni NTPDase Potato apyrase Peptide |
| title_short |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_full |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_fullStr |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_full_unstemmed |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_sort |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| author |
Mendes, Rita Gabriela Pedrosa Ribeiro |
| author_facet |
Mendes, Rita Gabriela Pedrosa Ribeiro Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila Faria Vasconcelos, Eveline Gomes |
| author_role |
author |
| author2 |
Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila Faria Vasconcelos, Eveline Gomes |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendes, Rita Gabriela Pedrosa Ribeiro Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila Faria Vasconcelos, Eveline Gomes |
| dc.subject.cnpq.fl_str_mv |
- |
| topic |
- Schistosoma mansoni NTPDase Potato apyrase Peptide |
| dc.subject.por.fl_str_mv |
Schistosoma mansoni NTPDase Potato apyrase Peptide |
| description |
- |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011 |
| dc.date.accessioned.fl_str_mv |
2019-01-15T14:11:59Z |
| dc.date.available.fl_str_mv |
2019-01-11 2019-01-15T14:11:59Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://repositorio.ufjf.br/jspui/handle/ufjf/8459 |
| dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762011000700005 |
| url |
http://dx.doi.org/10.1590/S0074-02762011000700005 https://repositorio.ufjf.br/jspui/handle/ufjf/8459 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.pt_BR.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
- |
| dc.publisher.initials.fl_str_mv |
- |
| dc.publisher.country.fl_str_mv |
Brasil |
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- |
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reponame:Repositório Institucional da UFJF instname:Universidade Federal de Juiz de Fora (UFJF) instacron:UFJF |
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Universidade Federal de Juiz de Fora (UFJF) |
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UFJF |
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UFJF |
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Repositório Institucional da UFJF |
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Repositório Institucional da UFJF |
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