Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência e Agrotecnologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400005 |
Resumo: | Germination of coffee seeds occurs in a slow and non-uniform manner and the tetrazolium test (TZ) may be a quick alternative for assessment of viability, as long as the preparation and imbibition of the seeds is established. This study was performed in two stages: in the first, recently harvested seeds from three coffee cultivars were submitted to four methodologies for removal of the parchment: manual extraction or use of sodium hypochlorite (NaClO at 5%) and two periods of imbibition of the seeds in water (36 and 48 hours) for removal of the embryos. In the second phase, manual removal and the use of NaClO were compared for elimination of the parchment of stored seeds for monthly performance of the tetrazolium test for six months. Sodium hypochlorite is not efficient in total extraction of the parchment and has a negative effect on the results of the TZ test on low moisture coffee seeds. An imbibition time of 48 hours facilitates extraction of embryos and does not affect test results. The tetrazolium test on coffee seeds with different moisture and deterioration levels must be undertaken with manual removal of the parchments. |
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Ciência e Agrotecnologia (Online) |
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Suitability of the tetrazolium test methodology for recently harvested and stored coffee seedsCoffea arabicaviabilityparchmentGermination of coffee seeds occurs in a slow and non-uniform manner and the tetrazolium test (TZ) may be a quick alternative for assessment of viability, as long as the preparation and imbibition of the seeds is established. This study was performed in two stages: in the first, recently harvested seeds from three coffee cultivars were submitted to four methodologies for removal of the parchment: manual extraction or use of sodium hypochlorite (NaClO at 5%) and two periods of imbibition of the seeds in water (36 and 48 hours) for removal of the embryos. In the second phase, manual removal and the use of NaClO were compared for elimination of the parchment of stored seeds for monthly performance of the tetrazolium test for six months. Sodium hypochlorite is not efficient in total extraction of the parchment and has a negative effect on the results of the TZ test on low moisture coffee seeds. An imbibition time of 48 hours facilitates extraction of embryos and does not affect test results. The tetrazolium test on coffee seeds with different moisture and deterioration levels must be undertaken with manual removal of the parchments.Editora da UFLA2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400005Ciência e Agrotecnologia v.36 n.4 2012reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542012000400005info:eu-repo/semantics/openAccessClemente,Aline da Consolação SampaioCarvalho,Maria Laene Moreira deGuimarães,Renato Mendeseng2012-08-13T00:00:00Zoai:scielo:S1413-70542012000400005Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:31:13.924175Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
title |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
spellingShingle |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds Clemente,Aline da Consolação Sampaio Coffea arabica viability parchment |
title_short |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
title_full |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
title_fullStr |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
title_full_unstemmed |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
title_sort |
Suitability of the tetrazolium test methodology for recently harvested and stored coffee seeds |
author |
Clemente,Aline da Consolação Sampaio |
author_facet |
Clemente,Aline da Consolação Sampaio Carvalho,Maria Laene Moreira de Guimarães,Renato Mendes |
author_role |
author |
author2 |
Carvalho,Maria Laene Moreira de Guimarães,Renato Mendes |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Clemente,Aline da Consolação Sampaio Carvalho,Maria Laene Moreira de Guimarães,Renato Mendes |
dc.subject.por.fl_str_mv |
Coffea arabica viability parchment |
topic |
Coffea arabica viability parchment |
description |
Germination of coffee seeds occurs in a slow and non-uniform manner and the tetrazolium test (TZ) may be a quick alternative for assessment of viability, as long as the preparation and imbibition of the seeds is established. This study was performed in two stages: in the first, recently harvested seeds from three coffee cultivars were submitted to four methodologies for removal of the parchment: manual extraction or use of sodium hypochlorite (NaClO at 5%) and two periods of imbibition of the seeds in water (36 and 48 hours) for removal of the embryos. In the second phase, manual removal and the use of NaClO were compared for elimination of the parchment of stored seeds for monthly performance of the tetrazolium test for six months. Sodium hypochlorite is not efficient in total extraction of the parchment and has a negative effect on the results of the TZ test on low moisture coffee seeds. An imbibition time of 48 hours facilitates extraction of embryos and does not affect test results. The tetrazolium test on coffee seeds with different moisture and deterioration levels must be undertaken with manual removal of the parchments. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-70542012000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da UFLA |
publisher.none.fl_str_mv |
Editora da UFLA |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.36 n.4 2012 reponame:Ciência e Agrotecnologia (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Ciência e Agrotecnologia (Online) |
collection |
Ciência e Agrotecnologia (Online) |
repository.name.fl_str_mv |
Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
||renpaiva@dbi.ufla.br|| editora@editora.ufla.br |
_version_ |
1799874968849219584 |