Morphology and pollen viability of Lolium multiflorum Lam.

Detalhes bibliográficos
Autor(a) principal: Nunes,Renata de Castro
Data de Publicação: 2012
Outros Autores: Bustamante,Fernanda de Oliveira, Techio,Vânia Helena, Mittelmann,Andréa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência e Agrotecnologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200006
Resumo: Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (> 89%) for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.
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spelling Morphology and pollen viability of Lolium multiflorum Lam.Scanning electron microscopyryegrassacetolysisStudy and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (> 89%) for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.Editora da UFLA2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200006Ciência e Agrotecnologia v.36 n.2 2012reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542012000200006info:eu-repo/semantics/openAccessNunes,Renata de CastroBustamante,Fernanda de OliveiraTechio,Vânia HelenaMittelmann,Andréaeng2012-05-22T00:00:00Zoai:scielo:S1413-70542012000200006Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:31:12.538588Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv Morphology and pollen viability of Lolium multiflorum Lam.
title Morphology and pollen viability of Lolium multiflorum Lam.
spellingShingle Morphology and pollen viability of Lolium multiflorum Lam.
Nunes,Renata de Castro
Scanning electron microscopy
ryegrass
acetolysis
title_short Morphology and pollen viability of Lolium multiflorum Lam.
title_full Morphology and pollen viability of Lolium multiflorum Lam.
title_fullStr Morphology and pollen viability of Lolium multiflorum Lam.
title_full_unstemmed Morphology and pollen viability of Lolium multiflorum Lam.
title_sort Morphology and pollen viability of Lolium multiflorum Lam.
author Nunes,Renata de Castro
author_facet Nunes,Renata de Castro
Bustamante,Fernanda de Oliveira
Techio,Vânia Helena
Mittelmann,Andréa
author_role author
author2 Bustamante,Fernanda de Oliveira
Techio,Vânia Helena
Mittelmann,Andréa
author2_role author
author
author
dc.contributor.author.fl_str_mv Nunes,Renata de Castro
Bustamante,Fernanda de Oliveira
Techio,Vânia Helena
Mittelmann,Andréa
dc.subject.por.fl_str_mv Scanning electron microscopy
ryegrass
acetolysis
topic Scanning electron microscopy
ryegrass
acetolysis
description Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (> 89%) for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1413-70542012000200006
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dc.publisher.none.fl_str_mv Editora da UFLA
publisher.none.fl_str_mv Editora da UFLA
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.36 n.2 2012
reponame:Ciência e Agrotecnologia (Online)
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
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reponame_str Ciência e Agrotecnologia (Online)
collection Ciência e Agrotecnologia (Online)
repository.name.fl_str_mv Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)
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