In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

Detalhes bibliográficos
Autor(a) principal: Silva,Tecla dos Santos
Data de Publicação: 2019
Outros Autores: Nepomuceno,Cristina Ferreira, Soares,Taliane Leila, Santana,José Raniere Ferreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência e Agrotecnologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100231
Resumo: ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.
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spelling In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditionsCatingueiraosmotic agentspaclobultrazoltissue culture.ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.Editora da UFLA2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100231Ciência e Agrotecnologia v.43 2019reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/1413-7054201943014519info:eu-repo/semantics/openAccessSilva,Tecla dos SantosNepomuceno,Cristina FerreiraSoares,Taliane LeilaSantana,José Raniere Ferreira deeng2019-12-04T00:00:00Zoai:scielo:S1413-70542019000100231Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:31:38.927577Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
title In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
spellingShingle In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
Silva,Tecla dos Santos
Catingueira
osmotic agents
paclobultrazol
tissue culture.
title_short In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
title_full In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
title_fullStr In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
title_full_unstemmed In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
title_sort In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions
author Silva,Tecla dos Santos
author_facet Silva,Tecla dos Santos
Nepomuceno,Cristina Ferreira
Soares,Taliane Leila
Santana,José Raniere Ferreira de
author_role author
author2 Nepomuceno,Cristina Ferreira
Soares,Taliane Leila
Santana,José Raniere Ferreira de
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,Tecla dos Santos
Nepomuceno,Cristina Ferreira
Soares,Taliane Leila
Santana,José Raniere Ferreira de
dc.subject.por.fl_str_mv Catingueira
osmotic agents
paclobultrazol
tissue culture.
topic Catingueira
osmotic agents
paclobultrazol
tissue culture.
description ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/1413-7054201943014519
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dc.publisher.none.fl_str_mv Editora da UFLA
publisher.none.fl_str_mv Editora da UFLA
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.43 2019
reponame:Ciência e Agrotecnologia (Online)
instname:Universidade Federal de Lavras (UFLA)
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reponame_str Ciência e Agrotecnologia (Online)
collection Ciência e Agrotecnologia (Online)
repository.name.fl_str_mv Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)
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