Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa

Detalhes bibliográficos
Autor(a) principal: Rong,Chengbo
Data de Publicação: 2021
Outros Autores: Song,Shuang, Yang,Li, Pan,Xuejiao, Liu,Yu, Wang,Shouxian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência e Agrotecnologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100227
Resumo: ABSTRACT Pholiota adiposa is a mushroom with excellent nutritional and medicinal properties. However, fruiting body yields are low, and the commercial cultivation potential of this fungus is limited. Here, 279 crossbred strains were obtained by the mono-mono crossing of monokaryotic strains derived from P. adiposa HS5 and HS4. Laccase enzymes and mycelial growth rates were used as markers to screen the crossbred strains, and 18 strains were selected for further analysis. Crossbred strain A10B4 displayed the highest yield (i.e. 165.91 ± 12.56 g per bag), which was 31.34 g and 74.48 g more than that of strains HS5 and HS4, respectively. The mycelial colonization time of A10B4 was 25.18 ± 1.33 d, which was 5.64 d shorter than that of HS5. A10B4 was characterized by inter-simple sequence repeat molecular markers. Differences in PCR products from parental and crossbred strains were observed. Therefore, the newly developed hybrid strain A10B4, named P. adiposa HS54 exhibiting high yield, might be suitable for commercial cultivation.
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spelling Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposaMushroomligninolytic enzymesmycelial growth.ABSTRACT Pholiota adiposa is a mushroom with excellent nutritional and medicinal properties. However, fruiting body yields are low, and the commercial cultivation potential of this fungus is limited. Here, 279 crossbred strains were obtained by the mono-mono crossing of monokaryotic strains derived from P. adiposa HS5 and HS4. Laccase enzymes and mycelial growth rates were used as markers to screen the crossbred strains, and 18 strains were selected for further analysis. Crossbred strain A10B4 displayed the highest yield (i.e. 165.91 ± 12.56 g per bag), which was 31.34 g and 74.48 g more than that of strains HS5 and HS4, respectively. The mycelial colonization time of A10B4 was 25.18 ± 1.33 d, which was 5.64 d shorter than that of HS5. A10B4 was characterized by inter-simple sequence repeat molecular markers. Differences in PCR products from parental and crossbred strains were observed. Therefore, the newly developed hybrid strain A10B4, named P. adiposa HS54 exhibiting high yield, might be suitable for commercial cultivation.Editora da UFLA2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100227Ciência e Agrotecnologia v.45 2021reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/1413-7054202145010921info:eu-repo/semantics/openAccessRong,ChengboSong,ShuangYang,LiPan,XuejiaoLiu,YuWang,Shouxianeng2021-10-26T00:00:00Zoai:scielo:S1413-70542021000100227Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:31:45.553319Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
title Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
spellingShingle Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
Rong,Chengbo
Mushroom
ligninolytic enzymes
mycelial growth.
title_short Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
title_full Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
title_fullStr Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
title_full_unstemmed Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
title_sort Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
author Rong,Chengbo
author_facet Rong,Chengbo
Song,Shuang
Yang,Li
Pan,Xuejiao
Liu,Yu
Wang,Shouxian
author_role author
author2 Song,Shuang
Yang,Li
Pan,Xuejiao
Liu,Yu
Wang,Shouxian
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rong,Chengbo
Song,Shuang
Yang,Li
Pan,Xuejiao
Liu,Yu
Wang,Shouxian
dc.subject.por.fl_str_mv Mushroom
ligninolytic enzymes
mycelial growth.
topic Mushroom
ligninolytic enzymes
mycelial growth.
description ABSTRACT Pholiota adiposa is a mushroom with excellent nutritional and medicinal properties. However, fruiting body yields are low, and the commercial cultivation potential of this fungus is limited. Here, 279 crossbred strains were obtained by the mono-mono crossing of monokaryotic strains derived from P. adiposa HS5 and HS4. Laccase enzymes and mycelial growth rates were used as markers to screen the crossbred strains, and 18 strains were selected for further analysis. Crossbred strain A10B4 displayed the highest yield (i.e. 165.91 ± 12.56 g per bag), which was 31.34 g and 74.48 g more than that of strains HS5 and HS4, respectively. The mycelial colonization time of A10B4 was 25.18 ± 1.33 d, which was 5.64 d shorter than that of HS5. A10B4 was characterized by inter-simple sequence repeat molecular markers. Differences in PCR products from parental and crossbred strains were observed. Therefore, the newly developed hybrid strain A10B4, named P. adiposa HS54 exhibiting high yield, might be suitable for commercial cultivation.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100227
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100227
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1413-7054202145010921
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da UFLA
publisher.none.fl_str_mv Editora da UFLA
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.45 2021
reponame:Ciência e Agrotecnologia (Online)
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Ciência e Agrotecnologia (Online)
collection Ciência e Agrotecnologia (Online)
repository.name.fl_str_mv Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv ||renpaiva@dbi.ufla.br|| editora@editora.ufla.br
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