'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência e Agrotecnologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
Resumo: | The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose. |
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'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storageEmbryo culturenutrient mediumbreedingThe aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose.Editora da UFLA2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029Ciência e Agrotecnologia v.30 n.5 2006reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542006000500029info:eu-repo/semantics/openAccessPasqual,MoacirDutra,Leonardo FerreiraAraujo,Aparecida Gomes deFigueiredo,Milene Alves deeng2007-11-06T00:00:00Zoai:scielo:S1413-70542006000500029Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2007-11-06T00:00Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
spellingShingle |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage Pasqual,Moacir Embryo culture nutrient medium breeding |
title_short |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_full |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_fullStr |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_full_unstemmed |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_sort |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
author |
Pasqual,Moacir |
author_facet |
Pasqual,Moacir Dutra,Leonardo Ferreira Araujo,Aparecida Gomes de Figueiredo,Milene Alves de |
author_role |
author |
author2 |
Dutra,Leonardo Ferreira Araujo,Aparecida Gomes de Figueiredo,Milene Alves de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pasqual,Moacir Dutra,Leonardo Ferreira Araujo,Aparecida Gomes de Figueiredo,Milene Alves de |
dc.subject.por.fl_str_mv |
Embryo culture nutrient medium breeding |
topic |
Embryo culture nutrient medium breeding |
description |
The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-70542006000500029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da UFLA |
publisher.none.fl_str_mv |
Editora da UFLA |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.30 n.5 2006 reponame:Ciência e Agrotecnologia (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Ciência e Agrotecnologia (Online) |
collection |
Ciência e Agrotecnologia (Online) |
repository.name.fl_str_mv |
Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
||renpaiva@dbi.ufla.br|| editora@editora.ufla.br |
_version_ |
1750226488605540352 |